A New HPLC-MS/MS Method for the Simultaneous Determination of Quercetin and Its Derivatives in Green Coffee Beans

Abstract

Green coffee (Coffee arabica and Coffee robusta) is one of the most commonly traded goods globally. Their beans are enriched with polyphenols and numerous health benefits are associated with their consumption. The main aim of this work was to develop a new and fast analytical HPLC-MS/MS method to simultaneously determine six flavonoid polyphenolic compounds (quercetin, rutin, isorhamnetin, quercetin-3-glucouronide, hyperoside, and quercitrin) in 22 green coffee samples from six different geographical origins (Ethiopia, Brazil, Guatemala, Nicaragua, India and Colombia). In addition, by adjusting pH, temperature, solvent type, and extraction duration, several extraction methods such as acidic and alkaline hydrolysis, and extraction without hydrolysis were evaluated. The optimal extraction procedure in terms of recovery percentages (78.67–94.09%)was acidic hydrolysis at pH 2, extraction temperature of 60 °C, extraction solvent of 70% ethanol, and extraction duration of 1.5 h. Hyperoside (878–75 μg/kg) was the most abundant compound followed by quercitrin (408–38 μg/kg), quercetin (300–36 μg/kg), rutin (238–21 μg/kg), and quercetin-3-glucouronide (225–7 μg/kg), while isorhamnetin (34–3 μg/kg) showed the lowest amount. Overall, green coffee beans are rich in flavonoid polyphenolic compounds and could be used as part of a healthy diet.

Quality evaluation methods of chinese medicine based on scientific supervision: recent research progress and prospects

“…Reversed phase-high performance liquid chromatography (RP-HPLC) is one of the most common determination methods, including HPLC with a diode array detector (HPLC–DAD) [ 12 ], HPLC with evaporative light scattering detector (HPLC-ELSD) [ 13 ], HPLC [ 14 ] with fluorescence detection (HPLC-FLD), HPLC-ultraviolet–visible-spectroscopy (HPLC–UV-Vis) [ 15 ]. Based on liquid chromatography [ 16 ] combined with other instrumental analyses are also applied to it, including HPLC-mass spectrometry technology (HPLC–MS) [ 17 ]. HPLC-high resolution MS and tandem MS (HPLC-HRMS/MS), ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) [ 18 ].…”

Enhanced Antioxidant Extraction from Lonicerae japonicae Flos Based on a Novel Optimization Strategy with Tailored Deep Eutectic Solvents

“…The standard curves of chlorogenic acid, rutin and 3,5-O-Dicaffeoylquinic acid; Table S12. The content of chlorogenic acid, rutin and 3,5-O-Dicaffeoylquinic acid in LJF and LF from different origins [38,47,48,[52][53][54][55][56][57].…”

Valorization of spent coffee ground and coffee silverskin as a source of nutrients and bioactive compounds

“…Coffee is one of the most consumed beverages in the world, and moreover, coffee beans are considered an important agricultural product of international trade (Mussatto et al., 2011; Mustafa et al., 2022). According to the latest statistics from the International Coffee Organization (ICO), global coffee consumption will reach 171.3 million bags in 2022–2023, with Europe accounting for about one‐third of global consumption (Tea and Coffee, 2023; ICO, 2023).…”