A New ImageJ Plug-in “ActogramJ” for Chronobiological Analyses

Schmid, Benjamin; Helfrich‐Förster, Charlotte; Yoshii, Taishi

doi:10.1177/0748730411414264

Cited by 294 publications

(241 citation statements)

References 11 publications

Supporting

Mentioning

241

Contrasting

Order By: Relevance

“…Raw data actograms were created using ActogramJ for ImageJ (Schmid et al, 2011). The average activity levels of all individual flies were calculated for the entire LD and DD period.…”

Section: Locomotor Activity Recording and Activity Analysismentioning

confidence: 99%

“…During the LD period, average activity levels were additionally determined during the first and second halves of the day and the night, respectively, to be compared with the sleep values during the same time periods (see below). To see whether GABA B -R2 downregulation affects the speed of the circadian clock, we determined the free-running periods of all individual flies during days 12 to 21 in DD by periodogram analysis (Sokolove and Bushell, 1978) combined with a chi-square test with a 5% significance level (Schmid et al, 2011).…”

Section: Locomotor Activity Recording and Activity Analysismentioning

confidence: 99%

See 1 more Smart Citation

GABAB receptors play an essential role in maintaining sleep during the second half of the night in Drosophila melanogaster

Gmeiner

Kołodziejczyk

Yoshii

et al. 2013

Journal of Experimental Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Raw data actograms were created using ActogramJ for ImageJ (Schmid et al, 2011). The average activity levels of all individual flies were calculated for the entire LD and DD period.…”

Section: Locomotor Activity Recording and Activity Analysismentioning

confidence: 99%

Section: Locomotor Activity Recording and Activity Analysismentioning

confidence: 99%

GABAB receptors play an essential role in maintaining sleep during the second half of the night in Drosophila melanogaster

Gmeiner

Kołodziejczyk

Yoshii

et al. 2013

Journal of Experimental Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…On day 8 (the second "transition day"), LL + UV conditions were reestablished at 9:00, and these conditions were then retained for another full week, to explore the possibility that any rhythmicity in the previous week might have been generated by masking. Data were collected in 60-min bins and actograms for each colony were plotted using the ActogramJ plug-in for ImageJ (National Institutes of Health, Bethesda, MD, USA) after normalization for variable colony size (Schmid et al, 2011). Period values (τ) and rhythmicity were also calculated in ActogramJ using χ 2 analysis.…”

Section: Data Analysis For Rfid-tagged Beesmentioning

confidence: 99%

Daily Changes in Ultraviolet Light Levels Can Synchronize the Circadian Clock of Bumblebees (Bombus terrestris)

Chittka

Stelzer

Stanewsky

2013

Chronobiology International

View full text Add to dashboard Cite

Endogenous circadian clocks are synchronized to the 24-h day by external zeitgebers such as daily light and temperature cycles. Bumblebee foragers show diurnal rhythms under daily light:dark cycles and short-period freerunning circadian rhythms in constant light conditions in the laboratory. In contrast, during the continuous light conditions of the arctic summer, they show robust 24-h rhythms in their foraging patterns, meaning that some external zeitgeber must entrain their circadian clocks in the presence of constant light. Although the sun stays above the horizon for weeks during the arctic summer, the light quality, especially in the ultraviolet (UV) range, exhibits pronounced daily changes. Since the photoreceptors and photopigments that synchronize the circadian system of bees are not known, we tested if the circadian clocks of bumblebees (Bombus terrestris) can be entrained by daily cycles in UV light levels. Bumblebee colonies were set up in the laboratory and exposed to 12 h:12 h UV + : UV− cycles in otherwise continuous lighting conditions by placing UV filters on their foraging arenas for 12 h each day. The activity patterns of individual bees were recorded using fully automatic radiofrequency identification (RFID). We found that colonies manipulated in such a way showed synchronized 24-h rhythms, whereas simultaneously tested control colonies with no variation in UV light levels showed free-running rhythms instead. The results of our study show that bumblebee circadian rhythms can indeed be synchronized by daily cycles in ambient light spectral composition. (Author correspondence: r.stanewsky@qmul.ac.uk)

show abstract

“…It is commercially available, easily modified and has been used in more than 600 publications. In addition, its adaptation for use in honey bees and other similar-sized insects would enable the use of multiple sophisticated data analysis tools that have been developed and validated throughout the years (Levine et al, 2002b;Gilestro and Cirelli, 2009;Schmid et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Measuring individual locomotor rhythms in honey bees, paper wasps and similar sized insects

Giannoni-Guzmán

Avalos

Perez

et al. 2014

Journal of Experimental Biology

View full text Add to dashboard Cite

Circadian rhythms in social insects are highly plastic and are modulated by multiple factors. In addition, complex behaviors such as sun-compass orientation and time learning are clearly regulated by the circadian system in these organisms. Despite these unique features of social insect clocks, the mechanisms as well as the functional and evolutionary relevance of these traits remain largely unknown. Here we show a modification of the Drosophila activity monitoring (DAM) system that allowed us to measure locomotor rhythms of the honey bee, Apis mellifera (three variants; gAHB, carnica and caucasica), and two paper wasps (Polistes crinitus and Mischocyttarus phthisicus). A side-by-side comparison of the endogenous period under constant darkness (free-running period) led us to the realization that these social insects exhibit significant deviations from the Earth's 24 h rotational period as well as a large degree of inter-individual variation compared with Drosophila. Experiments at different temperatures, using honey bees as a model, revealed that testing the endogenous rhythm at 35°C, which is the hive's core temperature, results in average periods closer to 24 h compared with 25°C (23.8 h at 35°C versus 22.7 h at 25°C). This finding suggests that the degree of tuning of circadian temperature compensation varies among different organisms. We expect that the commercial availability, cost-effectiveness and integrated nature of this monitoring system will facilitate the growth of the circadian field in these social insects and catalyze our understanding of the mechanisms as well as the functional and evolutionary relevance of circadian rhythms.

show abstract

A New ImageJ Plug-in “ActogramJ” for Chronobiological Analyses

Cited by 294 publications

References 11 publications

GABAB receptors play an essential role in maintaining sleep during the second half of the night in Drosophila melanogaster

GABAB receptors play an essential role in maintaining sleep during the second half of the night in Drosophila melanogaster

Daily Changes in Ultraviolet Light Levels Can Synchronize the Circadian Clock of Bumblebees (Bombus terrestris)

Measuring individual locomotor rhythms in honey bees, paper wasps and similar sized insects

Contact Info

Product

Resources

About