A new immobilization procedure for development of an electrochemical immunosensor for parathyroid hormone detection based on gold electrodes modified with 6-mercaptohexanol and silane

Şimşek, Çiğdem Sayıklı; Karaboğa, Münteha Nur Sonuç; Sezgintürk, Mustafa Kemal

doi:10.1016/j.talanta.2015.06.010

Cited by 30 publications

(10 citation statements)

References 25 publications

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“…In an attempt to develop improved PTH tests for the point-of-care in the operating theater, there have been some recent efforts to develop new diagnostic devices (Dittmer et al, 2008, Özcan and Sezgintürk, 2015, Sayikli ŞimŞek et al, 2015). Perhaps most notably, Philips has developed an intraoperative PTH assay based on their handheld magnotech technology (Bruls et al, 2009, Jarrige et al, 2011).…”

Section: Operating Roommentioning

confidence: 99%

Point-of-care diagnostics for niche applications

Cummins

Ligler

Walker

2016

Biotechnology Advances

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Point-of-care or point-of-use diagnostics are analytical devices that provide clinically relevant information without the need for a core clinical laboratory. In this review we define point-of-care diagnostics as portable versions of assays performed in a traditional clinical chemistry laboratory. This review discusses five areas relevant to human and animal health where increased attention could produce significant impact: veterinary medicine, space travel, sports medicine, emergency medicine, and operating room efficiency. For each of these areas, clinical need, available commercial products, and ongoing research into new devices are highlighted.

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Section: Operating Roommentioning

confidence: 99%

Point-of-care diagnostics for niche applications

Cummins

Ligler

Walker

2016

Biotechnology Advances

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“…Since functionalization of carboxyl groups via EDC/NHS with similar concentration were studied and optimized before [51,[57][58][59], optimization of the EDC/NHS concentration was not carried out. Moreover, optimum BSA concentration and duration of incubation were determined according to our previous work [57] and studies in the literature [60][61][62][63].…”

Section: Optimization Of Immunosensormentioning

confidence: 99%

Development of a functional impedimetric immunosensor for accurate detection of thy- roid-stimulating hormone

Asav¹

2021

Turk J Chem

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Thyroid-stimulating hormone (TSH) that regulates the synthesis of thyroid gland hormones controlling all metabolism is one of the pituitary hormones. Determination of TSH is crucial for monitoring thyroid gland-related disorders and some metabolic diseases.In this study, a non-labeled immunosensor based on covalent immobilization of anti-TSH antibody by using the formation of self-assembled monolayers (SAM) of 4mercaptophenylacetic acid (4-MPA) and functionalization of carboxyl ends with 1ethyl-3-(3-dimetilaminopropil) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) was fabricated for detection of TSH. Immobilization steps including the concentration of 4-2 MPA, the concentration of anti-TSH antibody and duration of anti-TSH antibody incubation were optimized by utilizing electrochemical impedance spectroscopy. Under optimal conditions, a sensitive, rapid and accurate determination of TSH at a concentration range between 0.7 -3.5 mIU/L was accomplished with a notable linearity and LOD value as 0.034 mIU/L as well as reproducibility and repeatability. Moreover, for comparison, linear range experiments were also carried out by using other electrochemical methods including Linear Sweep Voltammetry (LSV), Cyclic Voltammetry (CV) and capacitance spectroscopy (C). Finally, the constructed immunosensor was used for analyzing TSH levels spiked in the artificial serum samples.

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“…Taking advantages of label-free, simple, and fast analysis capabilities, EIS assays are widely utilized for the detection of small molecules, such as toxins, 125 hormones, 126 pesticides, 127 and feed additives. 128 For example, Shen and co-workers 129 established a simple immunosensor for testosterone detection based on an antitestosterone small single domain NB and EIS.…”

Section: ■ Immunosensors Based On Impedancementioning

confidence: 99%