A new immune resonance scattering spectral assay for trace fibrinogen with gold nanoparticle label

Jiang, Zhiliang; Sun, Shi‐Gang; Liang, Aihui; Chang-jiu, Liu

doi:10.1016/j.aca.2006.04.071

Cited by 23 publications

(9 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our group has accomplished a lot of work in this field. On the basis of the reaction of immunonanogold probe and antigen, which produced large immunocomplex, leading to the change of RS signal, the immunonanogold resonance scattering (INGRS) spectral methods for the detection of IgG [10], human chorionic gonadotrophin (hCG) [11], complement 3 [12], fibrinogen [13], APOA1 and APOB [14], prealbumin [15], Malb [16], IgA [17], etc., have been established successfully (Table 1). Compared to nanogold, nanosilver is of lower cost and easier to obtain.…”

Section: Nanogold and Nanosilver Particlesmentioning

confidence: 99%

Nanocatalytic resonance scattering spectral analysis

Jiang

Wen

et al. 2010

Sci. China Chem.

View full text Add to dashboard Cite

The resonance scattering spectral technique has been established using the synchronous scanning technique on spectrofluorometry. Because of its advantages of simplicity, rapidity and sensitivity, it has been widely applied to analyses of proteins, nucleic acids and inorganic ions. This paper summarizes the application of immunonanogold and aptamer modified nanogold (AptAu) catalytic resonance scattering spectral technique in combination with the work of our group, citing 53 references.antibody, aptamer, nanocatalysis, resonance scattering spectral assay

show abstract

Section: Nanogold and Nanosilver Particlesmentioning

confidence: 99%

Nanocatalytic resonance scattering spectral analysis

Jiang

Wen

et al. 2010

Sci. China Chem.

View full text Add to dashboard Cite

show abstract

“…To enhance the selectivity, some immunoreactions and catalytic reaction were combined with RS technique, with satisfactory results [18,19]. Recently, a study indicated that some inorganic nanoparticles such as gold and silver in liquid phase exhibited RS effect, which was applied to RS analysis of some antigen such as fibrinogen, apolipoprotein, and so on [20][21][22], with high sensitivity and good selectivity. In this work, using RS as a detection technique and combining the nanogold-labeled immunoreaction and immunonanogold catalytic reaction, a simple, rapid, sensitive, and selective immunonanogold-catalytic nanosilver RS assay for IgM was developed.…”

Section: Introductionmentioning

confidence: 99%

A Highly Sensitive Resonance Scattering Assay for Immunoglobulin M Using Ag(I)–Hydroquinone–Immunonanogold Catalytic Reaction

et al. 2008

View full text Add to dashboard Cite

Ten-nanometer nanogold showed the strongest catalytic effect on the particle reaction between Ag(I) and hydroquinone to form nanosilver particles that exhibited the strongest resonance scattering (RS) peak at 350 nm. The enhanced RS intensity was linear to the nanogold concentration in the range of 30-5,700 nM Au. The nanogold was used to label goat antihuman immunoglobulin M (GIgM) to obtain an immunonanogold probe (AuGIgM) for immunoglobulin M (IgM). Based on the nanogold-labeled immunoreaction between IgM and AuGIgM, centrifugation, and AuGIgM-Ag(I)-hydroquinone nanocatalytic reaction, a highly sensitive and selective immunonanogold-catalytic Ag particle RS assay for 0.2-300 ng mL −1 IgM was proposed, with a detection limit of 0.1 ng mL −1 . This assay was simple and sensitive and was applied to assay IgM in serum samples, with satisfactory results.

show abstract

“…The gold nanoparticle labelling technique, with high labelling effectiveness and the tiny effect on the activity of protein, has become the fourth labelling technique besides radioactive isotope, enzyme and fluorescent labelling, but has rarely been applied in quantitative analysis [12][13][14]. Recently, we combined the RS spectral technique and gold labelling technique to analyse the special proteins in the human body such as apolipoprotein, fibrinogen and so on, with simplicity, rapidity and good selectivity [15,16]. However, there is no report about the immunogold RS spectral probe for small molecular hapten.…”

Section: Introductionmentioning

confidence: 99%

A highly sensitive and selective immunonanogold resonance scattering spectral assay for sudan I

Jiang

Zou

Deng

et al. 2008

International Journal of Environmental Analytical Chemistry

View full text Add to dashboard Cite

A sensitive and selective resonance scattering spectral (RSS) assay was proposed for the determination of sudan I (SDI), using 10 nm nanogold to label the antibody against sudan I (anti-SDI Ab) to obtain a RSS probe for SDI. The immunonanogold reaction between nanogold-labelled anti-SDI Ab and SDI took place in pH 4.92 KH 2 PO 4 -Na 2 HPO 4 buffer solution and in the presence of polyethylene glycol (PEG)-6000, and the intensity of resonance scattering peak at 580 nm decreased greatly. The decreased intensity ÁI 580 nm was proportional to the concentration of SDI in the range of 0.23-45.0 ng mL À1 . The linear regression equation was calculated as ÁI 580nm ¼ 1.20c þ 2.01 (R ¼ 0.9975, n ¼ 6), with a detection limit (3) of 0.13 ng mL À1 . The SDI in egg samples was assayed, with satisfactory results.

show abstract

A new immune resonance scattering spectral assay for trace fibrinogen with gold nanoparticle label

Cited by 23 publications

References 19 publications

Nanocatalytic resonance scattering spectral analysis

Nanocatalytic resonance scattering spectral analysis

A Highly Sensitive Resonance Scattering Assay for Immunoglobulin M Using Ag(I)–Hydroquinone–Immunonanogold Catalytic Reaction

A highly sensitive and selective immunonanogold resonance scattering spectral assay for sudan I

Contact Info

Product

Resources

About