A new infrared fluorescent-Labeling agent and labeled antibody for diagnosing microcancers

Tadatsu, Masaya; Ito, Susumu; Muguruma, Naoki; Kusaka, Yoshiaki; Inayama, Kumi; Bando, Terumi; Tadatsu, Yoko; Okamoto, Koichi; Kunio,; Nagao, Yoshimitsu; Sano, Shigeki; Taue, Hiromi

doi:10.1016/s0968-0896(03)00239-6

Cited by 21 publications

(12 citation statements)

References 16 publications

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“…Tadatsu et al [10] present the development of indocyanine green derivatives used as an infrared fluorescent labelling substance for the detection of microlesions by IR fluorescence endoscopy. They show that indocyanine green derivatives with different concentrations in the range of 0.1ug/ml (∼129 nM) to 500 ug/ml (∼645 uM) can provide sufficient fluorescence intensity for diagnosis of microcancers.…”

Section: Capsule Testing and Resultsmentioning

confidence: 99%

A fluorescence based endoscopic microcancer detection capsule

Demosthenous

Georgiou

2015

2015 IEEE International Symposium on Circuits and Systems (ISCAS)

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Efficient microcancer detection in the small intestine can be realised by infrared fluorescence endoscopy (IRFE), in conjunction with an infrared fluorescent-labeling contrast agent that is designed to be selectively uptaken by cancerous cells. In this paper we present a second generation screening capsule, which is able to measure IR fluorescence levels emitted by the fluorophore indocyanine green (ICG), at different concentrations. The presented mixed-signal system is contained within a compact ingestible capsule, which is however large enough to come into close contact with the intestine walls during peristalsis. In-vitro experiments have shown that the system is able to detect and discriminate low concentrations of ICG, in the nanomolar region, which is required to detect early cancer in the small intestine.978-1-4799-8391-9/15/$31.00 ©2015 IEEE

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Section: Capsule Testing and Resultsmentioning

confidence: 99%

A fluorescence based endoscopic microcancer detection capsule

Demosthenous

Georgiou

2015

2015 IEEE International Symposium on Circuits and Systems (ISCAS)

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“…En permettant l'étude architecturale des tissus et des cellules de façon non invasive et en temps réel (biopsie optique), elle pourrait participer au dépistage de lésions précancéreuses, notamment cervicales et réduire le coût de leur dépistage. L'utilisation de nouveaux marqueurs fluorescents appelés quantum dots (fluorophores inorganiques formés de nanocristaux de semiconducteurs aux propriétés physiques intéressantes) et leur couplage, par exemple, à des anticorps monoclonaux [28] devraient permettre, dans un avenir proche, de compléter l'analyse morphologique des tissus cancéreux et fournir des indications sur l'expression de marqueurs moléculaires des tissus néoplasiques [9,29].…”

Section: Resultsunclassified

Place et intérêt de la microscopie confocale en gynécologie

Ascencio

Collinet

Cosson

et al. 2008

Journal de Gynécologie Obstétrique et Biologie de la Reproducti

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“…In contrast, other researchers reported a few limited successes in the literatures. Only a weak fluorescence was observed, when ICG-conjugated antibodies were applied for in vitro assays using sliced tissue samples, although the fluorescence yield of the ICG-conjugates were slightly improved by changing the chemical linkage (20). Withrow et al applied an ICG-conjugated antibody (cetuximab, anti-EGFR antibody) for imaging xenografted tumors in mice, however, they concluded that the sensitivity of this probe for cancer detection was low.…”

Section: Discussionmentioning

confidence: 99%

Dual-Modality Molecular Imaging Using Antibodies Labeled with Activatable Fluorescence and a Radionuclide for Specific and Quantitative Targeted Cancer Detection

et al. 2009

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Multimodality molecular imaging should have potential for compensating the disadvantages and enhancing the advantages of each modality. Nuclear imaging is superior to optical imaging in whole body imaging and in quantification due to good tissue penetration of gamma rays. However, target specificity can be compromised by high background signal due to the always signal ON feature of nuclear probes. In contrast, optical imaging can be superior in target specific imaging by employing target-specific signal activation systems, although it is not quantitative because of signal attenuation. In this study, to take advantage of the mutual cooperation of each modality, multimodality imaging was performed by a combination of quantitative radiolabeled probe and an activatable optical probe. The monoclonal antibodies, panitumumab (anti-HER1) and trastuzumab (anti-HER2) were labeled with 111 In and ICG, and tested in both HER1 and HER2 tumor bearing mice by the cocktail injection of radiolabeled and optical probes, and by the single injection of a dual-labeled probe. The optical and nuclear images were obtained over 6 days after the conjugates injection. The fluorescence activation properties of ICG labeled antibodies were also investigated by in vitro microscopy. In vitro microscopy demonstrated that there was no fluorescence signal with either panitumumab-ICG or trastuzumab-ICG, when the probes were bound to cell surface antigens but were not yet internalized. After the conjugates were internalized into the cells, both conjugates showed bright fluorescence signal only in the target cells. These results show both conjugates work as activatable probes. In vivo multimodality imaging by injection of a cocktail of radio-optical probes, only the target specific tumor was visualized by optical imaging. Meanwhile, the biodistribution profile of the injected antibody was provided by nuclear imaging. Similar results were obtained with radio and optical dual labeled probe, and it is confirmed that pharmacokinetic properties did not affect the results above.Here, we could characterize the molecular targets by activatable optical probes, and visualize the delivery of targeting molecules quantitatively by radioactive probes. Multimodality molecular imaging combining activatable optical and radioactive probe has great potential for simultaneous visualization, characterization, and measurement of biological processes.

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A new infrared fluorescent-Labeling agent and labeled antibody for diagnosing microcancers

Cited by 21 publications

References 16 publications

A fluorescence based endoscopic microcancer detection capsule

A fluorescence based endoscopic microcancer detection capsule

Place et intérêt de la microscopie confocale en gynécologie

Dual-Modality Molecular Imaging Using Antibodies Labeled with Activatable Fluorescence and a Radionuclide for Specific and Quantitative Targeted Cancer Detection

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