A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus

Granados, Robert R.; Li, Guoxun; Derksen, Anja C.G.; McKenna, Kevin A.

doi:10.1016/s0022-2011(94)90400-6

Cited by 197 publications

(132 citation statements)

References 27 publications

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“…AgMNPV isolate 2D ( Sieburth and Maruniak, 1988) was propagated in BTI-Tn-5B1-4 (Tn-5B) cells ( Granados et al, 1994) and maintained in TC-100 medium (GIBCO-BRL Life Technologies, Grand Island, NY, USA) supplemented with 10% fetal bovine serum at 27 °C. The inoculum was titered by the TCID50 method, following the protocol described by O'Reilly et al (1992).…”

Section: Insects and Virusesmentioning

confidence: 99%

Morphological characterization of Anticarsia gemmatalis M nucleopolyhedrovirus infection in haemocytes from its natural larval host, the velvet bean caterpillar Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae)

2004

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For a better understanding of virus×host interactions, transmission electron microscopy was used to characterize the intrahaemocoelic infection of Anticarsia gemmatalis larval haemocytes by A. gemmatalis M nucleopolyhedrovirus (AgMNPV). At 12 h post-infection (h p.i.), we observed nuclear hypertrophy, budded virus assembling, and protrusion towards the cytoplasm, virion envelopment, and accumulation of fibrillar aggregates in the cytoplasm. Around 24 h p.i., fibrillar aggregates also appeared inside nuclei of infected cells. By 48 h p.i., virogenic stroma and polyhedra were visualised in nuclei and at 72 h p.i., widespread infection in haemocytes was observed. Cell remnants and free polyhedra were phagocytosed by granular haemocyte 1 and plasmatocytes. Entire cells were phagocytosed only by plasmatocytes. Necrosis of infected cells was quite common, suggesting a putative cytotoxic response. Granular haemocyte 1 presented a more exuberant protrusion of budded viruses in comparison to other haemocytes. All types of haemocytes were shown to be infected, and the intense virus replication in some of these cells reveals the importance of haemolymph for AgMNPV spread in its natural host, a critical factor for permissiveness.

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Section: Insects and Virusesmentioning

confidence: 99%

Morphological characterization of Anticarsia gemmatalis M nucleopolyhedrovirus infection in haemocytes from its natural larval host, the velvet bean caterpillar Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae)

2004

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“…Bombyx mori Bm5 (Grace, 1967) and Trichoplusia ni Hi5 (BTI-TN-5B1-4) (Granados et al, 1994) insect cell lines were maintained in IPL-41 insect cell culture medium, supplemented with 10% fetal bovine serum (Life Technologies), at 28°C and sub cultured weekly. Bm5 and Hi5 cells were transfected following established protocols (Johnson et al, 1992).…”

Section: Cell Lines and Transfectionsmentioning

confidence: 99%

Abnormal development in larvae of Sesamia nonagrioides (Lepidoptera: Noctuidae) resulting from baculovirus-mediated overexpression of a JHE-related gene (SnJHER)

Kontogiannatos¹,

Swevers²,

Kourti³

2017

Eur. J. Entomol.

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Abstract. The Mediterranean corn borer Sesamia nonagrioides (Lepidoptera: Noctuidae) has a unique and recently multiplied juvenile hormone esterase gene family (SnJHER) with particular transcriptional profi les and functional characteristics. Unlike conventional juvenile hormone esterase genes (JHEs), the SnJHER gene family seems to have been recently evolved from a common ancestral JHER gene. SnJHERs seem to be regulated by both ecdysone agonists and xenobiotics, while their real role in development remains to be exploited. In this study we transiently expressed the major SnJHER isoform in Bm5 and Hi5 cell lines. The JHER-expressing cell lines showed increased toxicity when treated with the juvenile hormone analog methoprene. Moreover baculovirus-mediated transient gene transduction of the SnJHER gene in larvae of S. nonagrioides resulted in moulting abnormalities. These were more marked after the additional application of the juvenile hormone analog methoprene. Our results indicate a potential mechanism by which SnJHER interferes with normal JHE.

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“…Trichoplusia ni (BTI-Tn-5B1-4 or Tn-5B) cells ( Granados et al, 1994) were grown in TC-100 medium (Gibco-BRL) supplemented with 10% fetal calf serum at 27 °C. This cell line served as host for the in vitro propagation of AcMNPV, the polyhedra mutant virus and the recombinant virus constructed in this work.…”

Section: Cells and Virusesmentioning

confidence: 99%

Characterization of a new Autographa californica multiple nucleopolyhedrovirus (AcMNPV) polyhedra mutant

et al. 2009

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Characterization of a new Autographa californica multiple nucleopolyhedrovirus (AcMNPV) polyhedra mutantBergmann Morais Ribeiro Ana Paula Montenegro Generino Cláudia Natércia Lima Acacio Evanguedes Kalapothakis Sônia Nair Báo AbstractIn the very late phase of baculovirus infection, virions are occluded in a crystalline matrix called polyhedra, which is mainly composed of polyhedrin. This protein is highly conserved among baculoviruses and changes in its amino acid sequence may lead to mutant polyhedra. During the purification of an AcMNPV recombinant virus, a mutant virus was isolated. Structural and ultrastrutural analysis by light and transmission electron microscopy (TEM) of insect cells infected with this mutant virus did not show polyhedra formation and differed from the wild-type infection by the presence of a proteinaceous mass dispersed in the cytoplasm and nucleus of the infected cells, which was confirmed by immunogold labelling to be polyhedrin. The polyhedrin gene was amplified by PCR and sequenced. The only change observed was the substitution of a G to a T at the nucleotide +352, which resulted in a Val to Phe change. A recombinant virus was constructed by transferring the mutant gene into a polyhedrin negative virus. The phenotype of this recombinant virus was the same as the mutant one, confirming that this single mutation alone was responsible for the mutant phenotype.

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A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus

Cited by 197 publications

References 27 publications

Morphological characterization of Anticarsia gemmatalis M nucleopolyhedrovirus infection in haemocytes from its natural larval host, the velvet bean caterpillar Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae)

Morphological characterization of Anticarsia gemmatalis M nucleopolyhedrovirus infection in haemocytes from its natural larval host, the velvet bean caterpillar Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae)

Abnormal development in larvae of Sesamia nonagrioides (Lepidoptera: Noctuidae) resulting from baculovirus-mediated overexpression of a JHE-related gene (SnJHER)

Characterization of a new Autographa californica multiple nucleopolyhedrovirus (AcMNPV) polyhedra mutant

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