A new insight in desiccation tolerance and cryopreservation of mazzard cherry (<i>Prunus avium</i> L.) seeds

Michalak, Marcin; Plitta-Michalak, Beata Patrycja; Chmielarz, Paweł

doi:10.1515/biol-2015-0036

Cited by 11 publications

(8 citation statements)

References 31 publications

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“…Our study results are similar to those obtained by Hor et al (2005) for seeds of three different Citrus species, Dussert et al (2001) for seeds of five species of Coffea, and Michalak et al (2015aMichalak et al ( , 2015b for mazzard cherry (Prunus avium L.) and wild apple (Malus sylvestris) seeds. They observed that there was a decline in seed survival after liquid nitrogen exposure with dehydrating seeds to water content below the specific high-moisture freezing limit (MC around or under 20%).…”

Section: Acclimatization Of Cryopreserved Seedlingssupporting

confidence: 89%

Cryopreservation of citrus seed via dehydration followedby immersion in liquid nitrogen

Kaya¹,

Souza²,

Gökdoğan³

et al. 2017

Turk J Biol

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An important method for plant germplasm conservation is offered by a biotechnology-based approach of cryopreservation. Cryopreservation refers to the storage of plant material at ultralow temperatures in liquid nitrogen. A procedure for cryopreservation of polyembryonic seeds was improved for selected citrus cultivars from Turkey. Seed dehydration was performed at different exposure times, in sterile conditions of a laminar flow-hood. The tested cultivars showed the highest tolerance to low temperature storage when the seeds were first dehydrated to a moisture content of 21.8% for Poncirus trifoliata Raf. × Citrus sinensis Osb. and to 17.6% for Citrus limonia Osbeck. The postcryopreservation germinability ranged from 73.3% (Poncirus trifoliata Raf. × C. sinensis Osb. and Fortunella margarita (Lour.) Swingle) to 93.3% (C. jambhiri Lush.). Dehydration was beneficial for germination of seeds from all of the tested citrus species after the liquid nitrogen exposure. Seedlings derived from cryopreserved seeds had well-formed shoots and roots and were easily acclimated to greenhouse conditions.

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Section: Acclimatization Of Cryopreserved Seedlingssupporting

confidence: 89%

Cryopreservation of citrus seed via dehydration followedby immersion in liquid nitrogen

Kaya¹,

Souza²,

Gökdoğan³

et al. 2017

Turk J Biol

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“…Avoidance of intracellular ice formation during exposure to cryogenic temperatures is a prerequisite for successful cryopreservation of living organs, tissues, and cells (Endoh et al, 2018). The most critical factor affecting cryopreservation of seeds is MC (Michalak et al, 2015a;Verdier et al, 2013), so it should be determined for each species the accurate MC which allows seeds to tolerate cryostorage. Dehydration must be sufficient to avoid lethal intracellular freezing during cooling in LN but not so intense to induce extended desiccation injury.…”

Section: Discussionmentioning

confidence: 99%

Tolerance to Desiccation and Cryopreservation of Seeds of Seven South American Ilex Species

Dolce¹,

Medina²,

González-Arnao

2018

horts

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This research aimed at evaluating the desiccation tolerance and ability to withstand cryostorage of intact seeds of seven South American Ilex species, and comparing different methodologies for in vitro germination of fresh and cryostored seeds. Intact seeds were silica gel–desiccated from 2 to 14 hours, placed in cryovials, and immersed in liquid nitrogen (LN). Survival was assessed through in vitro germination of intact seeds, bisected seeds, or isolated embryos. Seeds of the seven Ilex species (Ilex brasiliensis (Sprengel) Loes., Ilex brevicuspis R., Ilex dumosa var. dumosa R., Ilex integerrima (Vell. Conc.) R., Ilex paraguariensis A. St. Hil., Ilex pseudoboxus R., and Ilex theezans R.) tolerated desiccation to ≈6% moisture content (MC) and could be successfully cryopreserved when MC decreased between 6.4% and 8.4% depending on the species, before immersion in LN. In addition, it was established as the optimal condition for in vitro seed germination of the seven Ilex species. A simple and cost-effective cryogenic procedure (which did not require the use of cryoprotectants or sophisticated facilities) was defined for seeds of seven Ilex species, which provides a new alternative for safe long-term preservation of Ilex germplasm.

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“…For other species that are also sensitive to dehydration and cooling, direct immersion in LN is the best option. Michalak, Plitta- Michalak and Chmielarz (2015) observed that cherry (Prunus avium L.) seeds tolerate cryopreservation when they are dehydrated in a range of moisture contents from 17%-20% wb and are directly immersed in LN.…”

Section: Biochemical and Ultrastructural Evaluationmentioning

confidence: 99%

Physiological, biochemical, and ultrastructural aspects of Coffea arabica L. seeds under different cryopreservation protocols

et al. 2021

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Cryopreservation is a technique that may potentially conserve the germplasm of species of the Coffea genus for an indeterminate time. The aim of this study was to evaluate the physiological, biochemical and ultrastructural characteristics of cryopreserved seeds of Coffea arabica L., cultivar Catucaí amarelo IAC 62, which was subjected to different protocols regarding dehydration, precooling, cooling, rewarming and cathode water use. According to each protocol, the seeds were subjected to fast or slow drying to moisture contents of 17 or 20% (wet basis), cooled in different ways, and then immersed in liquid nitrogen for 24 hours. Different rewarming times in a water bath were also used. Physiological, biochemical and ultrastructural analyses were performed on the seeds after the cryopreservation steps. Moisture content at a 17% wb is the key factor for the cryopreservation of Coffea arabica L. seeds, which have better physiological quality and better preserved cell structures. Precooling of coffee seeds before immersion in liquid nitrogen does not provide advantages compared to direct immersion. The rewarming times tested (2, 4, and 6 minutes) and cathode water use did not cause changes in the physiological and biochemical quality or in the cell structures of Coffea arabica L. cryopreserved seeds. The pattern of cell structure observed in all seeds indicates that the damage from cryopreservation is less drastic in the cells of the embryos than in those of the endosperm, with the latter less tolerant to the stresses of dehydration, precooling, and rewarming.

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A new insight in desiccation tolerance and cryopreservation of mazzard cherry (Prunus avium L.) seeds

Cited by 11 publications

References 31 publications

Cryopreservation of citrus seed via dehydration followedby immersion in liquid nitrogen

Cryopreservation of citrus seed via dehydration followedby immersion in liquid nitrogen

Tolerance to Desiccation and Cryopreservation of Seeds of Seven South American Ilex Species

Physiological, biochemical, and ultrastructural aspects of Coffea arabica L. seeds under different cryopreservation protocols

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