A new insight into the adsorption of bovine serum albumin onto porous polyethylene membrane by zeta potential measurements, FTIR analyses, and AFM observations

Xu, Tongwen; Fu, Rongqiang; Yan, Lifeng

doi:10.1016/s0021-9797(03)00208-x

Cited by 69 publications

(39 citation statements)

References 22 publications

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“…34,38 This interaction promotes condensation of silica around the protein, which in turn leads to film formation. Analysis of the PS-PANI-GDA-LYZ-SiG surface by AFM showed a coating of interconnected silica particles (1.054 AE 0.057 mm and $1.2 particles per nm 2 ) on the lysozyme treated surface with a Root Mean Square (RMS) roughness of 61.1 AE 3.3 nm (Fig.…”

Section: Growth and Characterisation Of Silica On Modified Polystyrenmentioning

confidence: 99%

Fabrication, characterisation and performance of hydrophilic and super-hydrophilic silica as cell culture surfaces

et al. 2012

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We demonstrate a straightforward procedure for the controlled formation of silica films on tissue culture polystyrene (PS) surfaces. The films were formed by sequentially treating PS with polyaniline, glutaric dialdehyde and protein prior to silica formation. The films could be tailored to exhibit superhydrophilicity (contact angle < 5 ) which was retained for more than two months under ambient conditions. Both hydrophilic and super-hydrophilic silica coated surfaces were suitable for the culture of an adherent human melanoma cell line. Proliferation, toxicity and adhesion assays were used to compare cell behaviour. Cells on the silica surfaces showed enhanced adhesion and comparable rates of cell proliferation as compared to cells grown on conventional tissue culture plastic. The results obtained can be understood by considering the surface properties of the different materials and the ability of the silica coated surfaces to adsorb significantly higher levels of serum proteins from the growth medium.One of the outcomes of this study is a re-evaluation of the hydrophobicity/hydrophilicity characteristics required for good cell growth and the possibility of designing new tissue culture materials capable of greater control over cell populations.

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Section: Growth and Characterisation Of Silica On Modified Polystyrenmentioning

confidence: 99%

Fabrication, characterisation and performance of hydrophilic and super-hydrophilic silica as cell culture surfaces

et al. 2012

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“…Two adsorption mechanisms of BSA, e.g., the side-on or the end-on adsorption with the major axes horizontal or perpendicular to the surface, have been discussed in the literature. [31][32][33] However, the formation of dimers, multilayers, or aggregates on the first, mostly irreversible bound, monolayer has also been observed. 34 Important factors that influence the architecture of the surface coating include the surface material, the concentration, the incubation time, and the buffer conditions.…”

Section: Resultsmentioning

confidence: 99%

“…This observation is in agreement with previous results where a time and concentration dependent adsorption model for native BSA on hydrophobic polyethylene surfaces and a critical concentration ͑1000 ppm, 0.06 mg/ml͒ were found. 33 BSA adsorption below the critical concentration proceeds in a normal side-on mechanism, whereas for higher concentrations, the adsorption proceeds in a crowded or aggregated way, which is similar to end-on, but with gaps between the adsorbed molecules.…”

Section: Fa83 Ritz Et Al: Cationized Albumin-biocoatings Fa83mentioning

confidence: 99%

Cationized albumin-biocoatings for the immobilization of lipid vesicles

et al. 2010

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Tethered lipid membranes or immobilized lipid vesicles are frequently used as biomimetic systems. In this article, the authors presented a suitable method for efficient immobilization of lipid vesicles onto a broad range of surfaces, enabling analysis by quantitative methods even under rigid, mechanical conditions-bare surfaces such as hydrophilic glass surfaces as well as hydrophobic polymer slides or metal surfaces such as gold. The immobilization of vesicles was based on the electrostatic interaction of zwitterionic or negatively charged lipid vesicles with two types of cationic chemically modified bovine serum albumin ͑cBSA͒ blood plasma proteins ͑cBSA-113 and cBSA-147͒. Quantitative analysis of protein adsorption was performed as the cBSA coatings were characterized by atomic force microscopy, surface zeta potential measurement, fluorescence microscopy, and surface plasmon spectroscopy, revealing a maximal surface coverage 270-280 ng/ cm 2 for 0.02 mg/ml cBSA on gold. Small unilamellar vesicles as well as giant unilamellar vesicles ͑GUVs͒ were readily immobilized ͑ϳ15 min͒ on cBSA coated surfaces. GUVs with 5-10 mol% negatively charged 1,2,-dipalmitoyl-sn-glycero-3-phosphoglycerol remained stable in liquid for at least 5 weeks.

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“…The presence of a large amount of BSA on the surface probably promotes fusion and interconnection of smaller silica particles via a sol-gel reaction to form the silica film [68]. Silica deposited on PEI/ODA-BSA coated surfaces ( Fig.…”

Section: Silica Film Formation Studymentioning

confidence: 99%

Fabrication of Tuneable Thickness Silica Films on Solid Surfaces Using Amines and Proteins

Rai

Perry

2009

Silicon

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We report an elegant and simple method to fabricate silica films with controlled thickness and roughness using protein coated solid surfaces as substrates. Bovine serum albumin (BSA) and lysozyme having different inherent charges have been used as model proteins (templates) to fabricate silica films. The formation of silica films was achieved by immobilization of BSA and lysozyme on amine (poly(allylamine) (PAH), poly(ethyleneimine) (PEI) and octadecyl amine (ODA)), coated surfaces, followed by treatment with silica precursors (tetramethoxysilane) under environmentally benign conditions of pH and temperature. BSA adsorbs strongly on hydrophilic surfaces (PAH and PEI coated) via electrostatic interaction, while lysozyme shows greater affinity towards hydrophobic surfaces (ODA coated) via largely hydrophobic interactions. The thickness (12-60 nm) and roughness of the films (1.30-3.75 nm) could be tuned by varying the amount of the adsorbed proteins on the amine-coated surfaces. This simple route to prepare silica films of controlled thickness could have potential application in membrane fabrication, biomedical devices, biosensors and next generation electronic components.

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A new insight into the adsorption of bovine serum albumin onto porous polyethylene membrane by zeta potential measurements, FTIR analyses, and AFM observations

Cited by 69 publications

References 22 publications

Fabrication, characterisation and performance of hydrophilic and super-hydrophilic silica as cell culture surfaces

Fabrication, characterisation and performance of hydrophilic and super-hydrophilic silica as cell culture surfaces

Cationized albumin-biocoatings for the immobilization of lipid vesicles

Fabrication of Tuneable Thickness Silica Films on Solid Surfaces Using Amines and Proteins

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