A new interface to couple thin‐layer chromatography with laser desorption/atmospheric pressure chemical ionization mass spectrometry for plate scanning

Peng, Song; Edler, Michael; Ahlmann, Norman; Hoffmann, Thorsten; Franzke, Joachim

doi:10.1002/rcm.2120

Cited by 45 publications

(39 citation statements)

References 16 publications

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“…Others have more recently used AP-LD/APCI for the analysis of analytes separated on TLC plates. 67 With the approach used by Harrison's group, an IR laser (10.6 µm) is used to desorb neutral molecules from a sample at AP, followed by ionization in the gas phase with corona discharge APCI. Secondary ionization by corona discharge APCI proved efficient, at least in part, because of the number of high density reagent ions in the region of the desorption plume.…”

Section: Laser Desorption Atmospheric Pressure Chemical Ionization (Lmentioning

confidence: 99%

Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry

2008

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The number and type of atmospheric pressure techniques suitable for sampling analytes from surfaces, forming ions from these analytes, and subsequently transporting these ions into vacuum for interrogation by MS have rapidly expanded over the last several years. Moreover, the literature in this area is complicated by an explosion in acronyms for these techniques, many of which provide no information relating to the chemical or physical processes involved. In this tutorial article, we sort this vast array of techniques into relatively few categories on the basis of the approaches used for surface sampling and ionization. For each technique, we explain, as best known, many of the underlying principles of operation, describe representative applications, and in some cases, discuss needed research or advancements and attempt to forecast their future analytical utility.

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Section: Laser Desorption Atmospheric Pressure Chemical Ionization (Lmentioning

confidence: 99%

Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry

2008

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“…Several studies have suggested the use of graphite as an alternative matrix for MALDI because it can disperse energy to an analyte extremely effectively . Graphite has been used in various forms; as a suspension, as a powder or as a surface . It is also a favourable choice for detection of LMW analytes because of the tendency of spectra to contain very few matrix peaks in the low‐mass region.…”

Section: Introductionmentioning

confidence: 99%

Investigation of colloidal graphite as a matrix for matrix‐assisted laser desorption/ionisation mass spectrometry of low molecular weight analytes

et al. 2016

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General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms MALDI, colloidal graphite, matrix suppression, small molecules, matrix. AbstractThe analysis of low molecular weight compounds by Matrix-Assisted Laser Desorption/Ionisation mass spectrometry is problematic due to the interference and suppression of analyte ionisation by the matrices typically employed -which are themselves low molecular weight compounds. The application of colloidal graphite is demonstrated here as an easy to use matrix that can promote the ionisation of a wide range of analytes including low molecular weight organic compounds, complex natural products and inorganic complexes. Analyte ionisation with colloidal graphite is compared with traditional organic matrices along with various other sources of graphite (e.g. graphite rods and charcoal pencils). Factors such as ease of application, spectra reproducibility, spot longevity, spot-to-spot reproducibility and spot homogeneity (through single spot imaging) are explored. For some analytes considerable matrix suppression effects are observed resulting in spectra completely devoid of matrix ions. We also report the observation of radical molecular ions [M -• ] in the negative ion mode, particularly with some aromatic analytes.

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“…It is possible that the esters spread out as a result of adding the 2,6-DHB matrix solution because lateral spreading of analytes, caused by using a liquid matrix, has been observed. 10,17,31 However, no ester signal was observed at the edges of the sample spots unless coincident with the pencil line. This suggested that the pencil lead was in fact a more suitable matrix than 2,6-DHB.…”

Section: Resultsmentioning

confidence: 96%

Detection of Long Alkyl Esters of Succinic and Maleic Acid Using TLC-MALDI-MS

Kim¹,

Han²,

Kim³

et al. 2011

Bulletin of the Korean Chemical Society

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Four esters of succinic and maleic acid were synthesized, separated by thin-layer chromatography (TLC), and identified using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). A comparison of matrix materials showed that 2,6-dihydroxybenzoic acid (2,6-DHB) yielded a greater ionization efficiency than 2,5-DHB prior to TLC separation. The location of each ester sample on the TLC plate was estimated by comparing the developed plate with a duplicate plate that had been visualized by immersion in a KMnO4 solution. Generally, mass spectra obtained from the KMnO4-visualized plate were relatively poor. Reproducible mass spectra with high peak abundance were difficult to obtain using the 2,6-DHB matrix from crude synthetic esters extracted from the TLC plates. Significant improvements in both reproducibility and sensitivity were realized by using pencil lead as the MALDI matrix. The current methodology will be beneficial to organic chemists since it can provide a guideline for simple and rapid characterization of small organic compounds.

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A new interface to couple thin‐layer chromatography with laser desorption/atmospheric pressure chemical ionization mass spectrometry for plate scanning

Cited by 45 publications

References 16 publications

Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry

Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry

Investigation of colloidal graphite as a matrix for matrix‐assisted laser desorption/ionisation mass spectrometry of low molecular weight analytes

Detection of Long Alkyl Esters of Succinic and Maleic Acid Using TLC-MALDI-MS

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