A new label-free fluorescent sensor for human immunodeficiency virus detection based on exonuclease III-assisted quadratic recycling amplification and DNA-scaffolded silver nanoclusters

Yang, Wen; Tian, Jianniao; Wang, Lijun; Fu, Shui; Huang, Hongyun; Zhao, Yi; Zhao, Shulin

doi:10.1039/c6an00184j

Cited by 17 publications

(2 citation statements)

References 34 publications

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“…The intensity of the fluorescence alterations decreased with the increase in the number of mutations. Yang and coworkers developed a fluorescence multi-amplified biosensor for the label-free diagnosis of HIV-DNA based on the DNA/Ag NCs fluorescence and Exo III-assisted cyclic amplification strategy [78]. Three types of DNA were employed in this approach.…”

Section: Fluorescence Assays Based On Metallic Nanoparticles Nanoclusters and Nanosheetsmentioning

confidence: 99%

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Nanotechnology based strategies for HIV-1 and HTLV-1 retroviruses gene detection

Mozhgani

Kermani

Norouzi

et al. 2020

Heliyon

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Early detection of retroviruses including human T-cell lymphotropic virus and human immunodeficiency virus in the human body is indispensable to prevent retroviral infection propagation and improve clinical treatment. Until now, diverse techniques have been employed for the early detection of viruses. Traditional methods are timeconsuming, resource-intensive, and laborious performing. Therefore, designing and constructing a selective and sensitive diagnosis system to detect serious diseases is highly demanded. Genetic detection with high sensitivity has striking significance for the early detection and remedy of disparate pathogenic diseases. The nucleic acid biosensors are based on the identification of specific DNA sequences in biological samples. Nanotechnology has an important impact on the development of sensitive biosensors. Different kinds of nanomaterials include nanoparticles, nanoclusters, quantum dots, carbon nanotubes, nanocomposites, etc., with different properties have been used to improve the performance of biosensors. Recently, DNA nanobiosensors are developed to provide simple, fast, selective, low-cost, and sensitive detection of infectious diseases. In this paper, the research progresses of nano genosensors for the detection of HIV-1 and HTLV-1 viruses, based on electrochemical, optical, and photoelectrochemical platforms are overviewed.

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Section: Fluorescence Assays Based On Metallic Nanoparticles Nanoclusters and Nanosheetsmentioning

confidence: 99%

“…Representation of the analysis of the HIV-DNA using DNA/AgNCs and autonomous exonuclease III (Exo III)-assisted recycling signal amplification, A) Fluorescence on; B) Fluorescence off. Reproduced from [78], with permission from the Royal Society of Chemistry. the background signal.…”

Section: Fluorescence Polarization Assaymentioning

confidence: 99%