A New LC–MS/MS Bioanalytical Method for Atenolol in Human Plasma and Milk

Lwin, Ei Mon Phyo; Gerber, Jacobus P.; Song, Yunmei; Leggett, Catherine; Ritchie, Usha; Turner, Sean; Garg, Sanjay

doi:10.4155/bio-2016-0304

Cited by 12 publications

(6 citation statements)

References 15 publications

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“…Several clean-up methods such as liquid-liquid extraction [3, 11, 12], solid phase extraction [21], and protein precipitation [22] have been used for extracting ATN from biological fluid samples.…”

Section: Introductionmentioning

confidence: 99%

Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method

El-Alfy

Ismaiel

El-Mammli

et al. 2019

International Journal of Analytical Chemistry

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A simple RP-HPLC-PDA method for determination of atenolol (ATN) and trimetazidine (TMZ) in human urine and tablets has been developed. Analytes were separated on a Caltrex BI column (125× 4.0 mm, 5 μm) with 25mM potassium dihydrogen phosphate pH 3.3, methanol, and acetonitrile mobile phases. The PDA detector was operated at 210 nm for TMZ and 225 nm for ATN and the flow rate was 1.0 mL/ min. Linearity was obtained over a concentration range of (1.0-100 μg/mL) for both analytes in standard solutions and the method was successfully applied for determination of target analytes in their pharmaceutical tablets. Excellent linearity was also obtained over concentration ranges of (0.25-25 μg/mL) and (0.5-25 μg/mL) in human urine for TMZ and ATN, respectively. A simple liquid-liquid extraction was applied for urine sample clean-up and a gradient method was used for chromatographic separation. The lower limit of quantitation (LOQ) was 0.99 and 0.60 μg/mL for ATN and TMZ, respectively. The limit of detection (LOD) was 0.30 and 0.18 μg/mL for ATN and TMZ, respectively. Inter- and intraday precision and accuracy for ATN were within ±1.89% in pure form and within ±2.85% in urine samples. Inter- and intraday precision and accuracy for TMZ were within ± 3.99% in pure form and within ± 3.19% in urine samples.

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Section: Introductionmentioning

confidence: 99%

Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method

El-Alfy

Ismaiel

El-Mammli

et al. 2019

International Journal of Analytical Chemistry

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“…The spiked samples were mixed thoroughly by vortex mixing for approximately 1 minute. Our previously developed protein precipitation and ultracentrifugation methods 3 , 9 , 10 were used for the separation of rosuvastatin from biological samples. Drug-free human plasma and milk samples were used as negative controls.…”

Section: Methodsmentioning

confidence: 99%

Transfer of rosuvastatin into breast milk: liquid chromatography–mass spectrometry methodology and clinical recommendations

Lwin¹,

Leggett²,

Ritchie³

et al. 2018

DDDT

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IntroductionRosuvastatin reduces concentrations of total cholesterol (TC) and is used for the management of hypercholesterolemia and prevention of acute coronary syndromes. There are no published reports estimating infant exposure to rosuvastatin through breast milk.PurposeThe aims of this study were to quantify concentrations of rosuvastatin in human milk and plasma from a lactating woman taking rosuvastatin and to investigate potential infant exposure.Materials and methodsA 38-year-old breastfeeding mother was commenced on rosuvastatin 20 mg daily for secondary prevention of an acute coronary syndrome. Eight maternal breast milk samples and a single plasma sample were collected over a 24-hour period. The samples were quantified using a sensitive liquid chromatography–mass spectrometry (LC-MS/MS) method.ResultsThe average concentration of rosuvastatin in breast milk was 30.84 ng/mL, and a peak concentration of 58.59 ng/mL occurred at 17 hours after oral administration. Although the milk-to-plasma (M/P) ratio was 16.49 at 14 hours, the theoretical infant dosage (TID) and relative infant dose (RID) were 0.005 mg/kg/day and 1.50%, respectively.ConclusionThe findings suggest that only small amounts of rosuvastatin pass into breast milk. Should the maternal condition necessitate treatment, consideration could be given to the use of rosuvastatin during breastfeeding provided the infant is monitored.

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“…The materials used, preparation of the stock solutions, standard curve solutions, test samples, and the assay method are as described in our method development and validation article (Lwin et al, 2017). The method was reliable and adequate since a linearity range of 1 to 800 ng/mL ( r 2 = .9995), the precision within 15% CV, and the recovery of the analyte (80%-100% range) were achieved using a Phenomenex C-18 column and gradient chromatographic conditions.…”

Section: Methodsmentioning

confidence: 99%

“…Subsequent milk samples were collected in the participants' homes using prelabeled containers. Milk samples were stored in the home refrigerator and returned to the Women's and Children's Hospital within 24 hr and then stored in an −80°C freezer until analysis, in accordance with our previous stability studies (Lwin et al, 2017). .…”

Section: Data Collectionmentioning

confidence: 99%

Estimation of Atenolol Transfer Into Milk and Infant Exposure During Its Use in Lactating Women

et al. 2018

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A New LC–MS/MS Bioanalytical Method for Atenolol in Human Plasma and Milk

Abstract: A new validated analytical method for atenolol in plasma and milk was developed.

Cited by 12 publications

References 15 publications

Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method

Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method

Transfer of rosuvastatin into breast milk: liquid chromatography–mass spectrometry methodology and clinical recommendations

Estimation of Atenolol Transfer Into Milk and Infant Exposure During Its Use in Lactating Women

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A New LC–MS/MS Bioanalytical Method for Atenolol in Human Plasma and Milk

Abstract: A new validated analytical method for atenolol in plasma and milk was developed.

Cited by 12 publications

References 15 publications

Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method

Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method

Transfer of rosuvastatin into breast milk: liquid chromatography&ndash;mass spectrometry methodology and clinical recommendations

Estimation of Atenolol Transfer Into Milk and Infant Exposure During Its Use in Lactating Women

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Transfer of rosuvastatin into breast milk: liquid chromatography–mass spectrometry methodology and clinical recommendations