A New Lipase Isolated from Oleaginous Seeds from Pachira aquatica (Bombacaceae)

Abstract

A new lipase from seeds of Pachira aquatica was purified to homogeneity by SDS-PAGE obtaining an enzyme with a molecular weight of approximately 55 kDa. The purified lipase exhibited maximum activity at 40 degrees C and pH 8.0, for an incubation time of 90 min. Concerning temperature stability, at the range from 4 to 50 degrees C, it retained approximately 47% of its original activity for 3 h. The enzyme activity increased in the presence of Ca(++) and Mg(++), but was inhibited by Hg(++), Mn(++), Zn(++), Al(+++) and various oxidizing and reducing agents. The lipase was highly stable in the presence of organic solvents, and its activity was stimulated by methanol. The values of K(m) and V(max) were 1.65 mM and 37.3 micromol mL(-1) min(-1), respectively, using p-nitrophenylacetate as substrate. The enzyme showed preference for esters of long-chain fatty acids, but demonstrated significant activity against a wide range of substrates.

Stability of a Lipase Extracted from Seeds of Pachira aquatica in Commercial Detergents and Application Tests in Poultry Wastewater Pretreatment and Fat Particle Hydrolysis

“…Nevertheless, recently, seed lipases have been the focus of increasing attention as biocatalysts. In some cases, these enzymes present advantages over animal and microbial lipases due to some very interesting features such as specificity, low cost, availability, and easily purification, representing a great alternative for potential commercial use as industrial enzymes [4–6]. …”

“…However, their single biggest market is detergent formulations. The functional importance of lipases in detergent industries is to remove fatty residues in laundry and dishwashers, for cleaning clogged drains, and for avoiding pipes' clogging [4, 19–21]. Some studies have been done adding lipases to detergent formulations or adding these detergents to lipase assays to evaluate enzyme stability [4, 9, 20].…”

Caracterização do óleo das sementes de Pachira aquatica Aublet para aproveitamento alimentar

“…Segundo Cetto e Heinrich (2005), o consumo de Pachira aquática aublet pode ter efeito hipoglicemiante no tratamento de diabetes e seus sintomas. em estudos realizados por Polizelli et al (2008) e Lago et al (1987) foram constatados elevado teor de óleo (44,1%) no fruto de Pachira aquática aublet, sendo o ácido palmítico o seu principal componente. Polizelli et al (2008) também observaram elevado teor de triptofano, aminoácido precursor de um importante neurotransmissor, a serotonina.…”

“…em estudos realizados por Polizelli et al (2008) e Lago et al (1987) foram constatados elevado teor de óleo (44,1%) no fruto de Pachira aquática aublet, sendo o ácido palmítico o seu principal componente. Polizelli et al (2008) também observaram elevado teor de triptofano, aminoácido precursor de um importante neurotransmissor, a serotonina. oliveira et al (2000), estudando a composição e as propriedades nutricionais de sementes, encontraram teores de 53,9% de óleo e 12,9% de proteínas.…”

Cloning, Sequence Analysis and Expression Patterns during Seed Germination of a Rapeseed (Brassica napus L.) G-x-S-x-G-motif Lipase Gene

“…During germination and cotyledon senescence, oil bodies are degraded by lipases, which mobilize stored TAGs (Vijayakumar and Gowda, 2012). It has been reported that, with a few exceptions, lipase activity is absent in ungerminated (or dormant) seeds and increases rapidly upon germination (e.g., Peres Polizelli et al, 2008). In some cases, enzymes isolated from dormant seeds -for example from castor bean (Estamond, 2004), peanut (Sanders and Pattee, 1975), or Jatropha curcas (Abigor et al, 2002) -are highly active in vitro but apparently inactive in vivo.…”