A New Medium for the Isolation and Growth of Auxotrophic Mutants of the Blue-green Alga Anacystis nidulans

A Synechocystis 6714 mutant resistant to the phenol-type herbicide ioxynil was isolated and characterized. Sensitivity to DCMU and atrazine was measured in whole cells and isolated thylakoids. The mutant presents the same sensitivity to atrazine as the wild type and a slightly increased sensitivity to DCMU. A point mutation has been found at codon 266 in the psbAI coding locus (AAC to ACC) resulting in an amino acid change from asparagine to threonine in the D 1 protein.

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“…[20] with twice the concentration of nitrate. For the solid medium, 1.5°70 agar autoclaved separately was added.…”

Section: Growth Conditionsmentioning

confidence: 99%

Mutation in phenol‐type herbicide resistance maps within thepsbA gene inSynechocystis6714

et al. 1989

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“…Cells were maintained axenically on 1.4% agar slants made with Allen's medium (1) and kept under low light (15 uE m-2 s-') provided by a combination of warm white and Gro-lux fluorescent lamps (Sylvania, Canada). Single cell isolates, obtained by dilution plating on the minimal medium of Herdman et al (15), were used in all studies.…”

Section: Methodsmentioning

confidence: 99%

Growth and Photosynthesis of the Cyanobacterium Synechococcus leopoliensis in HCO₃⁻-Limited Chemostats

Miller

Turpin²,

Canvin³

1984

Plant Physiol.

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SyecococcEs lkopoliensis was grown in HCO03-limited chemostats.Growth at 50% the maximum rate occurred when the inorganic carbon concentration was 10 to 15 micromolar (or 5.6 to 8. nanomolar C02). The 02 to CO2 ratios during growth were as high as 192,000 to 1. At growth rates below 80% the maximum rate, essentially all the supplied inorganic carbon was converted to organic carbon, and the cells were carbon limited. Carbon-limited cells used HCO3-rather than CO2 for growth. They also exhibited a very high photosynthetic affinity for inorgnic carbon in short-term experiments. Cells growing at greater than 80% maximum growth rate, in the presence of high dissolved inorganic carbon, were termed carbon sufficient. These cells had photosynthetic affinities that were about 1000-fold lower than HCO3-limited cells and also had a reduced capacity for HC03-transport. HCO3--limited cells are reminiscent of the air-grown cells of batch culture studies while the carbon sufficient cells are reminiscent of high-CO2 grown cells. However, the low affinity cells of the present study were growing at CO2 concentrations less than air saturation. This suggests that supranormal levels of CO2 not required to induce the physiological changes usually ascribed to high CO2 cells.

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“…strain PCC 6803 was grown photoautotrophically under continuous fluorescent illumination (25 W. m -2, white light) at 35°C on a synthetic medium [6] Glutamate dehydrogenase aminating activity was determined at 30°C in a reaction mixture containing 85 mM Tris-HC1 buffer (pH 8.0), 10 mM 2-oxoglutarate, 50 mM NH4CI and 0.2 mM NADPH. The reaction was started by addition of ammonium chloride and followed either spectrophotometrically at 340 nm or by glutamate formation.…”

Section: Growth Of Cellsmentioning

confidence: 99%

Identification and characterization of a glutamate dehydrogenase in the unicellular cyanobacterium Synechocystis PCC 6803

1987

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Glutamate dehydrogenase activity has been detected in permeabilized cells of the unicellular cyanobacterium Synechocystis PCC 6803 at similar levels to those of glutamate synthase. The enzyme responsible has been purified by affinity chromatography on 2',5'-ADP-Sepharose and identified as an NADPH-specific glutamate dehydrogenase. The enzyme catalyzes preferentially glutamate formation rather than the reverse reaction, with Km values for NADPH, 2-oxoglutarate and ammonia of 20/aM, 1.5 mM and 3.7 mM, respectively. It is composed of four identical subunits giving a total molecular mass of 208 kDa for the native protein. Its physiological role is discussed in terms of being an alternative pathway to the glutamine synthetase-glutamate synthase cycle for ammonia assimilation.

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A New Medium for the Isolation and Growth of Auxotrophic Mutants of the Blue-green Alga Anacystis nidulans

Cited by 90 publications

References 9 publications

Mutation in phenol‐type herbicide resistance maps within thepsbA gene inSynechocystis6714

Mutation in phenol‐type herbicide resistance maps within thepsbA gene inSynechocystis6714

Growth and Photosynthesis of the Cyanobacterium Synechococcus leopoliensis in HCO₃⁻-Limited Chemostats

Identification and characterization of a glutamate dehydrogenase in the unicellular cyanobacterium Synechocystis PCC 6803

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A New Medium for the Isolation and Growth of Auxotrophic Mutants of the Blue-green Alga Anacystis nidulans

Cited by 90 publications

References 9 publications

Mutation in phenol‐type herbicide resistance maps within thepsbA gene inSynechocystis6714

Mutation in phenol‐type herbicide resistance maps within thepsbA gene inSynechocystis6714

Growth and Photosynthesis of the Cyanobacterium Synechococcus leopoliensis in HCO3−-Limited Chemostats

Identification and characterization of a glutamate dehydrogenase in the unicellular cyanobacterium Synechocystis PCC 6803

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Growth and Photosynthesis of the Cyanobacterium Synechococcus leopoliensis in HCO₃⁻-Limited Chemostats