A New Method for Determination of Inorganic Phosphorits in Serum without Deproteinization

El‐Merzabani, Mahmoud M.; Anwer-El-Aaser, Abdelbaset; Zakhary, Nadia I.

doi:10.1515/cclm.1977.15.1-12.715

Cited by 35 publications

(32 citation statements)

References 9 publications

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“…Serum levels of calcium and phosphorus were determined according to Gindler and King, (1972) and El-Merzabani et al, (1977), respectively. Additionally, serum PTH was estimated through radioim munoassay (RIA) according to Deftos, (1990).…”

Section: Chemical Analysismentioning

confidence: 99%

Effect of Moringa Leaves and Seeds on Osteoporosis in Rats

Habib

Al-Moalem

2018

Journal of Food and Dairy Sciences

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Osteoporosis (OP) speaks to most regular metabolic bone maladies. This study was conducted to study the impact of Moringa oleifera leaves and seeds and their blends on osteoporosis in rats. Thirty five adult female albino of rats, of (180 ± 10 g) were divided into five groups, the first group, negative control group (-ve) and fed on basal diet only, the other four groups (7 rats each) were fed on basal diet containing 100 mg Prednisone Acetate as source of glucocorticoid/ kg diet to induce osteoporosis for two weeks. One group of them was served as a positive control group, the other three groups were fed on prednisone acetate diets containing dried moringa leaves (2.5%), moringa seeds (2.5%) and 2.5% of their combination at (1:1), respectively. Blood samples and femur bones were collected for estimating both serum and bone markers of osteoporosis. The results indicated that, supplementation with Moringa oleifera leaves and seeds and their combinations significantly increased (P<0.05) serum Ca and P in osteoporotic rats. There was significant increase (P<0.05) in serum free thyroxin (T4) and a significant decrease (P<0.05) in parathormone (PTH) in osteoporotic rats. Femur bone mineral density (BMD) were also significantly increased. The present examination suggested utilizing Moringa oleifera leaves and seeds and their blends as a good anti-osteoporotic activity.

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Section: Chemical Analysismentioning

confidence: 99%

Effect of Moringa Leaves and Seeds on Osteoporosis in Rats

Habib

Al-Moalem

2018

Journal of Food and Dairy Sciences

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“…Calcium and phosphorus contents in serum and tibial bone were measured according to Gindler and King (1972) and El-Merzabani et al (1977), respectively. Serum total alkaline phosphatase (TALP) and bone alkaline phosphatase (BALP) were determined colorimetrically according to the method described by Belfield and Goldberg (1971).…”

Section: Biochemical Analysesmentioning

confidence: 99%

Antiosteoporotic effect of Coelatura aegyptiaca shell powder on ovariectomized rats

Sayed¹,

Soliman²,

Fahmy³

et al. 2013

Afr. J. Pharm. Pharmacol.

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The efficacy of Coelatura aegyptiaca shell powder (CES, 500 mg/kg BW) was evaluated as a calcium supplement in ovariectomized (OVX) rats for ten weeks of treatment. The biochemical components and the antioxidant properties of the shell powder were determined. The bone mineral density (BMD), bone mineral content (BMC), calcium (Ca) and phosphorus (P) contents in serum and bone, serum total alkaline phosphatase (TALP) and bone alkaline phosphatase (BALP), serum calcitonin and parathyroid (PTH) hormones were determined. Furthermore, some of the oxidative stress markers [malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidant capacity (TAC)] were estimated in bone. The current study revealed that CES contained 19.38% Ca, 0.315% P as well as some of antioxidant amino acids which have a potent antioxidant activity against 1,1-diphenylpicrylhydrazyl (DPPH) free radical. Administration of CES to OVX rats increased BMD, BMC, tibial Ca and P contents and BALP activity, as compared to OVX rats. An ameliorative effect was recorded in the levels of calcitonin, PTH, MDA, SOD, GPx and TAC subsequent to CES administration to OVX rats.This ameliorative effect of CES powder against osteoporosis may be attributed to its antioxidant efficacy and/or to its Ca content. In conclusion, CES may have the potential to develop a clinically useful anti-osteoporotic agent, since its effect was comparable with alendronate (6.5 mg/kg BW/week).

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“…Serum levels of calcium and phosphorus were determined according to Gindler and King, (1972) and El-Merzabani et al, (1977), respectively. Additionally, serum parathyroid hormone (PTH) was estimated through enzyme linked immunosorbent assay (ELISA), according to Norazlina et al, (2010).…”

Section: Chemical Analysismentioning

confidence: 99%

The Possible Protective Role of Powder Cuttlefish Bone, Crabshell and Eggshell on Osteoporotic Rats

Negm

2018

Journal of Food and Dairy Sciences

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Bone mass declines alongside maturing, particularly for ladies after menopause as a result of diminishing estrogen emission together with low calcium consumption. This examination was directed to examine the impact of powdered cuttlebone, crab shell and eggshell, on female rats with induced osteoporosis. Fifty adult female albino rats, of (200 ± 10 g) were haphazardly divided into five groups, the first group, negative control group (-ve) fed on basal diet only, the other four groups (10 rats each) were fed on basal diet containing 100 mg prednisone acetate as source of glucocorticoid/ kg diet to induce osteoporosis for two weeks. One group of them was selected as a positive control group, the other three groups were fed on prednisone acetate diets containing powder of cuttlebone, crabshell and eggshell, at the level of 2.5% individually. Blood samples and femur bones were gathered for measure both serum and bone markers of osteoporosis. The results indicated that, supplementation with powder of cuttlebone, crabshell and eggshell significantly (P<0.05) increase of Ca and P in serum of osteoporotic rats. There were a significant increment in serum free thyroxin (T4) and a significant decrease in parathyroid hormone (PTH). Femur bone mineral content (BMC) and bone mineral density (BMD) were likewise increased as compared to positive control group. In addition, liver functions were significantly (P<0.05) improved compared to the positive control group, while serum lipid profiles were significantly decreased. These results recommend that sustaining postmenopausal rats with osteoporosis on diets supplemented with powder of cuttlebone, crab shell and eggshell instigated an enemy of osteoporotic impact. This impact may be due to upgrade of bone arrangement and direction of some metabolic hormones which control calcium. Therefore, it could be recommended using powder of cuttlebone, crabshell and eggshell for postmenopausal women suffering from osteoporosis.

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A New Method for Determination of Inorganic Phosphorits in Serum without Deproteinization

Cited by 35 publications

References 9 publications

Effect of Moringa Leaves and Seeds on Osteoporosis in Rats

Effect of Moringa Leaves and Seeds on Osteoporosis in Rats

Antiosteoporotic effect of Coelatura aegyptiaca shell powder on ovariectomized rats

The Possible Protective Role of Powder Cuttlefish Bone, Crabshell and Eggshell on Osteoporotic Rats

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