A new method for karyological studies in teleost fishes

Cucchi, C.; Baruffaldi, A.

doi:10.1111/j.1095-8649.1990.tb05928.x

Cited by 32 publications

(21 citation statements)

References 16 publications

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“…Chromosome preparations were obtained from the anterior kidney cells after in vivo treatment with colchicine (Bertollo et al, 1978). Some specimens were previously treated with yeast suspension (Lee and Elder, 1980), or cobalt chloride solution (Cucchi and Baruffaldi, 1990) for mitotic stimulation. In addition to conventional Giemsa staining, the chromosomes were analyzed after Mithramycin (Schmid, 1980) and Distamycin/…”

Section: Citogenética Evolutiva Do Grupo Hoplias Lacerdae Um Comportmentioning

confidence: 99%

Evolutionary cytogenetics of the Hoplias lacerdae, Miranda Ribeiro, 1908 group: a particular pathway concerning the other Erythrinidae fish

2007

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The taxonomy/systematics of the Erythrinidae fish is still imprecise, with several doubts on their relationships. Karyotypes and chromosomal characteristics of some species of the Hoplias lacerdae group (Erythrinidae), from different Brazilian hydrographic basins and pisciculture stations, were analyzed in the present study, using conventional Giemsa staining, C-banding, silver staining, Mithramycin and Distamycin/DAPI fluorochromes, and fluorescent in situ hybridization (FISH). A diploid chromosome number of 2n = 50 and karyotypes composed of meta-and submetacentric chromosomes without sex-related differences were found. Only one active NOR (Nucleolar Organizer Region) site was found, which was identified by silver staining (Ag-NOR) and FISH, located on the chromosome pair 11, although additional 45S rDNA sites were also mapped on other chromosome pairs only by FISH. The Ag-NOR of the chromosome pair 11 was found to be GC-rich, appearing positive after Mithramycin staining. Mithramycin-positive/ DAPI-negative sites were also observed in the centromeric/pericentomeric regions of the chromosome pairs 4, 6, 15, and 19, which have also affinity to silver nitrate. However, these four sites were not detected by FISH with the rDNA probe, indicating to be only argentophilic GC-rich heterochromatic regions. Chromosome data show that the karyotype evolution in Hoplias lacerdae group is relatively conserved and follows a particular pathway concerning the other Erythrinidae fishes, such as Hoplias malabaricus, Hoplerythrinus unitaeniatus, and Erythrinus erythrinus, in which polytypic karyotypes are found. Thus, the H. lacerdae group shows chromosome features that are not closely related to those of the congeneric H. malabaricus group. These finds, together with genetic and morphologic data, are important tools to be considered in a major revision of the Erythrinidae family, as well as for conservation programs.Keywords: erythrinid, chromosome features, karyotypic evolution, cytotaxonomy. Citogenética evolutiva do grupo Hoplias lacerdae. Um comportamento particular em relação aos outros peixes eritrinídeos ResumoA taxonomia dos Erythrinidae encontra-se ainda por ser totalmente esclarecida, existindo várias dúvidas sobre suas relações filogenéticas. No presente estudo, foram analisados os cariótipos e características cromossômicas de algumas espécies do grupo Hoplias lacerdae (Erythrinidae), de diferentes bacias hidrográficas do Brasil e estações de piscicultura, empregando-se a coloração Giemsa convencional, bandamento C, coloração com nitrato de prata, fluorocromos Mitramicina e Distamicina/DAPI, e hibridação fluorescente in situ (FISH). O número diplóide de 2n = 50 cromossomos foi constante, sendo os cariótipos formados por cromossomos de dois braços, meta-e submetacêntricos, sem diferenciação relacionada ao sexo. Foi identificado apenas um sítio ativo de região organizadora de nucléolo (NOR), presente no par cromossômico no. 11, identificado tanto pela coloração com nitrato de prata (Ag-NOR) como por FISH com son...

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Section: Citogenética Evolutiva Do Grupo Hoplias Lacerdae Um Comportmentioning

confidence: 99%

Evolutionary cytogenetics of the Hoplias lacerdae, Miranda Ribeiro, 1908 group: a particular pathway concerning the other Erythrinidae fish

2007

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“…Topical treatment with cobalt chloride solution demonstrated that this compound is a potent inducer of metaphase in larvae and pupae of Melipona. Cucchi and Baruffaldi (1990) recommended an injection of 500 mL 65 mM cobalt chloride solution per 100 g body weight in fish. We opted for topical application, and the quantity that produced the best results in bees was 4 mL 65 mM cobalt chloride solution per individual.…”

Section: Resultsmentioning

confidence: 99%

“…The technique described for fish by Cucchi and Baruffaldi (1990) was adapted to bees. We applied 4.0 mL 65 mM cobalt chloride solution topically in the dorsal region of defecating larvae and pupae [white-eyed pupa (Pw), pink-eyed pupa (Pp), brown-eyed pupa (Pb), brown-eyed pupa with light pigmented body (Pbl), black-eyed pupa with pigmented body (Pbd)] of M. scutellaris.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, in the present study we tested a simple methodology using cobalt chloride modified from that for fish described by Cucchi and Baruffaldi (1990). This method produced a high number of metaphases in larvae and pupae of M. scutellaris stingless bee, and it is a simple, fast, and efficient means of increasing metaphases in cytogenetic preparations.…”

Section: Introductionmentioning

confidence: 99%

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Cobalt chloride induces metaphase when topically applied to larvae and pupae of the stingless bee Melipona scutellaris (Hymenoptera, Apidae, Meliponini)

Ueira-Vieira

Tavares²,

Morelli³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. In order to optimize preparations of bee metaphases, we tested cobalt chloride, which has been used as a metaphase inducer in other organisms, such as hamsters and fish. Four microliters of 65 mM cobalt chloride aqueous solution was topically applied to larval and pupal stages of the stingless bee Melipona scutellaris. The cerebral ganglion was removed after treatment and prepared for cytogenetic analysis. Identically manipulated untreated individuals were used as controls. The number of metaphases was increased 3-fold in treated individuals compared to controls. The micronucleus test showed no mutagenic effects of cobalt chloride on M. scutellaris cells. We concluded that cobalt chloride is a metaphase-inducing agent in M. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (2): 2032-2037 (2013 Cobalt chloride induces metaphase in stingless bees 2033 scutellaris, thus being useful for cytogenetic analyses.

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“…The technique of mitotic stimulation in vertebrates relies on the immunological response to antigens, which can be obtained from a wide variety of sources: live microorganisms such as yeasts (Lee and Elder, 1980;Lozano et al, 1988); biological compounds such as horse serum (Ojima and Kurishita, 1980) and phytohemagglutinin, a lectin found in plants able to induce mitosis (Baruffaldi et al, 1992;Takai and Ojima, 1995); natural or induced infection with parasites such as Ichthiophirium multifilis (Moreira-Filho and Bertollo, 1991) and parasitic mollusk larvae (Woznicki et al, 2004), and chemical agents such as phenylhydrazine (Cucchi and Baruffaldi, 1989) and cobalt chloride (Cucchi and Baruffaldi, 1990;Woznicki et al 2004). All these methods trigger, to different degrees, the proliferation of defense cells, which is macroscopically characterized by an enlarged kidney, the hematopoietic organ in fish (Kunz, 2004).…”

Section: Introductionmentioning

confidence: 99%

Performance of human immunostimulating agents in the improvement of fish cytogenetic preparations

Molina

Alves²,

Araújo³

et al. 2010

Genet. Mol. Res.

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A new method for karyological studies in teleost fishes

Cited by 32 publications

References 16 publications

Evolutionary cytogenetics of the Hoplias lacerdae, Miranda Ribeiro, 1908 group: a particular pathway concerning the other Erythrinidae fish

Evolutionary cytogenetics of the Hoplias lacerdae, Miranda Ribeiro, 1908 group: a particular pathway concerning the other Erythrinidae fish

Cobalt chloride induces metaphase when topically applied to larvae and pupae of the stingless bee Melipona scutellaris (Hymenoptera, Apidae, Meliponini)

Performance of human immunostimulating agents in the improvement of fish cytogenetic preparations

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