A new method for spectrophotometric determination of carbaryl based on rubber tree bark peroxidase enzymatic reaction

Supharoek, Sam-ang; Ponhong, Kraingkrai; Siriangkhawut, Watsaka; Grudpan, Kate

doi:10.1016/j.microc.2018.08.048

Cited by 13 publications

(10 citation statements)

References 32 publications

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“…The green color of ABTS cation radical was observed, indicating that peroxidase was found in the crude extracts from cassia bark. Activity of the crude enzyme was 1.15 ± 0.05 kU 10 μL −1 , equivalent to 0.32 kU L −1 of horseradish peroxidase (HRP, Sigma-Aldrich, St. Louis, MO, USA) evaluated from HRP activity calibration in the range 0.125–2.0 kU L −1 [ 52 ]. Peroxide activity remained constant for 3 months at −20 °C.…”

Section: Resultsmentioning

confidence: 99%

“…Sodium chloride was added to improve extraction efficiency through the salting-out effect by decreasing the solubility of the analyte [ 52 , 63 ]. Results are shown in Figure 3 e. The signal sharply increased from 0.6 to 1.0% ( w / v ) and then reduced from 1.2 to 1.4% ( w / v ).…”

Section: Resultsmentioning

confidence: 99%

“…Enzymes are often employed as bio-accelerators because they require small amounts of substrate and utilize specific reactions that operate under mild conditions [ 50 , 51 ]. Peroxidase enzymatic spectrophotometry has been used to quantify carbaryl residues in vegetables by our group [ 52 , 53 ]. Cassia bark ( Senna simea ), an indigenous plant found in many areas including Thailand, Southern India, Sri Lanka, Myanmar (Burma), Cambodia, Malaysia and parts of Indonesia was employed as a source of peroxidase enzyme for extraction by a simple method yielding crude peroxidase enzyme.…”

Section: Introductionmentioning

confidence: 99%

“…However, a sample containing carbaryl at low levels in complicated matrices requires pretreatment before the colorimetric detection step. Sample pretreatment techniques such as dispersive liquid-liquid microextraction (DLLME) have recently attracted increased attention to concentrate the sample before analysis [ 52 , 57 , 58 ]. DLLME has advantages of simplicity of operation, rapidity, low cost, high recovery, high preconcentration factor and environmental benignity [ 59 ].…”

Section: Introductionmentioning

confidence: 99%

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A Simple and Reliable Dispersive Liquid-Liquid Microextraction with Smartphone-Based Digital Images for Determination of Carbaryl Residues in Andrographis paniculata Herbal Medicines Using Simple Peroxidase Extract from Senna siamea Lam. Bark

et al. 2022

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A simple and reliable dispersive liquid-liquid microextraction (DLLME) coupled with smartphone-based digital images using crude peroxidase extracts from cassia bark (Senna siamea Lam.) was proposed to determine carbaryl residues in Andrographis paniculata herbal medicines. The method was based on the reaction of 1-naphthol (hydrolysis of carbaryl) with 4-aminoantipyrine (4-AP) in the presence of hydrogen peroxide, using peroxidase enzyme simple extracts from cassia bark as biocatalysts under pH 6.0. The red product, after preconcentration by DLLME using dichloromethane as extraction solvent, was measured for blue intensity by daily life smartphone-based digital image analysis. Under optimized conditions, good linearity of the calibration graph was found at 0.10–0.50 mg·L−1 (r2 = 0.9932). Limits of detection (LOD) (3SD/slope) and quantification (LOQ) (10SD/slope) were 0.03 and 0.09 mg·L−1, respectively, with a precision of less than 5%. Accuracy of the proposed method as percentage recovery gave satisfactory results. The proposed method was successfully applied to analyze carbaryl in Andrographis paniculata herbal medicines. Results agreed well with values obtained from the HPLC-UV method at 95% confidence level. This was simple, convenient, reliable, cost-effective and traceable as an alternative method for the determination of carbaryl.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Simple and Reliable Dispersive Liquid-Liquid Microextraction with Smartphone-Based Digital Images for Determination of Carbaryl Residues in Andrographis paniculata Herbal Medicines Using Simple Peroxidase Extract from Senna siamea Lam. Bark

et al. 2022

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“…Supharoek, Ponhong, Siriangkhawut, and Grudpan (2019) achieved spectrophotometric analysis of carbaryl in vegetables, based on the enzymatic reaction of peroxidase derived from rubber tree bark. SPE coupled with DLLME was used for carbaryl concentration, followed by UV‐vis spectrophotometry.…”

Section: Spectrophotometric Techniquesmentioning

confidence: 99%

Analytical methods to analyze pesticides and herbicides

Begum

Xue

2020

Water Environment Research

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This paper reviews studies published in 2019, in the area of analytical techniques for determination of pesticides and herbicides. It should be noted that some of the reports summarized in this review are not directly related to but could potentially be used for water environment studies. Based on different methods, the literatures are organized into six sections, namely extraction methods, electrochemical techniques, spectrophotometric techniques, chemiluminescence and fluorescence methods, chromatographic and mass spectrometric techniques, and biochemical assays.

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A Cost-Effective Spectrophotometric Method Based on Enzymatic Analysis of Jackfruit Latex Peroxidase for the Determination of Carbaryl and Its Metabolite 1-Napthol Residues in Organic and Chemical-Free Vegetables

et al. 2019

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A new method for spectrophotometric determination of carbaryl based on rubber tree bark peroxidase enzymatic reaction

Cited by 13 publications

References 32 publications

A Simple and Reliable Dispersive Liquid-Liquid Microextraction with Smartphone-Based Digital Images for Determination of Carbaryl Residues in Andrographis paniculata Herbal Medicines Using Simple Peroxidase Extract from Senna siamea Lam. Bark

A Simple and Reliable Dispersive Liquid-Liquid Microextraction with Smartphone-Based Digital Images for Determination of Carbaryl Residues in Andrographis paniculata Herbal Medicines Using Simple Peroxidase Extract from Senna siamea Lam. Bark

Analytical methods to analyze pesticides and herbicides

A Cost-Effective Spectrophotometric Method Based on Enzymatic Analysis of Jackfruit Latex Peroxidase for the Determination of Carbaryl and Its Metabolite 1-Napthol Residues in Organic and Chemical-Free Vegetables

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