A new mode of mitochondrial transport and polarized sorting regulated by Dynein, Milton and Miro

Melkov, Anna; Baskar, Raju; Alcalay, Yehonatan

doi:10.1242/jcs.199653

Cited by 3 publications

(3 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kinesin family-1 (KIF5), a predominately neuronal kinesin motor, attaches to microtubules via adaptor proteins. Trak 1/2 are prominent adaptor proteins in neurons ( Iworima et al, 2016 ; Melkov et al, 2016 ). Trak1/2 attach KIF5 to microtubules by binding the KIF5 cargo-binding domain and the outer mitochondrial membrane receptor known as Mitochondrial Rho-GTPase (Miro) ( Lee and Lu, 2014 ; López-Doménech et al, 2018 ).…”

Section: Mitochondrial Transport Is Altered In Models Of Peripheral Neuropathymentioning

confidence: 99%

HDAC6: A Key Link Between Mitochondria and Development of Peripheral Neuropathy

English

Barton

2021

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Peripheral neuropathy, which is the result of nerve damage from lesions or disease, continues to be a major health concern due to the common manifestation of neuropathic pain. Most investigations into the development of peripheral neuropathy focus on key players such as voltage-gated ion channels or glutamate receptors. However, emerging evidence points to mitochondrial dysfunction as a major player in the development of peripheral neuropathy and resulting neuropathic pain. Mitochondrial dysfunction in neuropathy includes altered mitochondrial transport, mitochondrial metabolism, as well as mitochondrial dynamics. The mechanisms that lead to mitochondrial dysfunction in peripheral neuropathy are poorly understood, however, the Class IIb histone deacetylase (HDAC6), may play an important role in the process. HDAC6 is a key regulator in multiple mechanisms of mitochondrial dynamics and may contribute to mitochondrial dysregulation in peripheral neuropathy. Accumulating evidence shows that HDAC6 inhibition is strongly associated with alleviating peripheral neuropathy and neuropathic pain, as well as mitochondrial dysfunction, in in vivo and in vitro models of peripheral neuropathy. Thus, HDAC6 inhibitors are being investigated as potential therapies for multiple peripheral neuropathic disorders. Here, we review emerging studies and integrate recent advances in understanding the unique connection between peripheral neuropathy and mitochondrial dysfunction through HDAC6-mediated interactions.

show abstract

Section: Mitochondrial Transport Is Altered In Models Of Peripheral Neuropathymentioning

confidence: 99%

HDAC6: A Key Link Between Mitochondria and Development of Peripheral Neuropathy

English

Barton

2021

Front. Mol. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Mitochondrial Rho GTPases (miro) interact with microtubule motors kinesin (Fransson et al, 2006) and dynein (Morlino et al, 2014), as well as adapter protein Milton (Morlino et al, 2014;Melkov et al, 2016), to facilitate bidirectional transport of mitochondria (Guo et al, 2005;Russo et al, 2009). We assessed the role of microtubule motors in the apical mitochondrial transport during cellularization using an RNAi-based approach to knock down Miro (miro i ) using nanos-Gal4.…”

Section: Mitochondrial Redistribution During Cellularization Is Regulated By Microtubule-based Transportmentioning

confidence: 99%

Mitochondrial morphology and activity regulate furrow ingression and contractile ring dynamics inDrosophilacellularization

Chowdhary

Madan

Tomer

et al. 2020

MBoC

View full text Add to dashboard Cite

Mitochondria are maternally inherited in many organisms. Mitochondrial morphology and activity regulation is essential for cell survival, differentiation and migration. An analysis of mitochondrial dynamics and function in morphogenetic events in early metazoan embryogenesis has not been carried out. In our study we find a crucial role of mitochondrial morphology regulation in cell formation in Drosophila embryogenesis. We find that mitochondria are small, fragmented and translocate apically on microtubules and distribute progressively along the cell length during cellularization. Embryos mutant for mitochondrial fission protein, Drp1, die in embryogenesis and show an accumulation of clustered mitochondria on the basal side in cellularization. Additionally, Drp1 mutant embryos contain lower levels of reactive oxygen species (ROS). ROS depletion has been previously shown to decrease Myosin II activity. Drp1 loss also leads to Myosin II depletion at the membrane furrow thereby resulting in decreased cell height and larger contractile ring area in cellularization similar to Myosin II mutants. The mitochondrial morphology and cellularization defects in Drp1 mutants are suppressed by reducing mitochondrial fusion and increasing cytoplasmic ROS in superoxide dismutase mutants. Our data show a key role for mitochondrial morphology and activity in supporting the morphogenetic events that drive cellularization in Drosophila embryos. [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text]

show abstract

“…Among the insect‐specific actin‐associated protein is Javelin (Shapira et al, 2011). A second group of genes that affect bristle development are associated with MT organization, which includes mainly MT motor proteins Kinesin heavy chain (Brendza et al, 2000), Kinesin light chain (Melkov et al, 2015) and Dynein heavy chain 64C (Melkov et al, 2016). The third group includes genes that are part of the endosomal pathway, namely, Rab11 (Nagaraj & Adler, 2012), Dusky‐like (Nagaraj & Adler, 2012) and Rab35 (Zhang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Scale‐type‐specific requirement for the mosquito Aedes aegyptiSpindle‐F homologue by regulating microtubule organization

Djokic

Bakhrat

et al. 2021

Insect Molecular Biology

Self Cite

View full text Add to dashboard Cite

Insect epithelial cells contain unique cellular extensions such as bristles, hairs, and scales. In contrast to bristle and hair, which are not divergent in their shape, scale morphology shows high diversity. In our attempt to characterize the role of the insect‐specific gene, Spindle‐F (spn‐F), in mosquito development, we revealed a scale‐type specific requirement for the mosquito Aedes aegypti spn‐F homologue. Using CRISPR‐Cas9, we generated Ae‐spn‐F mutants and found that Ae‐spn‐F is an essential gene, but we were able to recover a few adult escapers. These escapers could not fly nor move, and died after 3 to 4 days. We found that in Ae‐spn‐F mutants, only the tip part of the bristle was affected with bulbous with misoriented ribs. We also show that in Ae‐spn‐F mutants, only in falcate scales, which are curved with a sharp or narrowly rounded apex, and not in other scale types, the tip region is strongly affected. Our analysis also revealed that in contrast to Drosophila spn‐F, which show strong defects in both the actin and microtubule (MT) network in the bristle, the Ae‐spn‐F gene is required only for MT organization in scales and bristles. In summary, our results reveal that Ae‐spn‐F is required for shaping tapered epithelial cellular extension structures, namely, the bristle and falcate scales by affecting MT organization.

show abstract

A new mode of mitochondrial transport and polarized sorting regulated by Dynein, Milton and Miro

Cited by 3 publications

References 1 publication

HDAC6: A Key Link Between Mitochondria and Development of Peripheral Neuropathy

HDAC6: A Key Link Between Mitochondria and Development of Peripheral Neuropathy

Mitochondrial morphology and activity regulate furrow ingression and contractile ring dynamics inDrosophilacellularization

Scale‐type‐specific requirement for the mosquito Aedes aegyptiSpindle‐F homologue by regulating microtubule organization

Contact Info

Product

Resources

About