A New Mutation in Wheat Producing Three Pistils in a Floret

Peng, Zhengsong

doi:10.1046/j.1439-037x.2003.00040.x

Cited by 32 publications

(38 citation statements)

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“…It is clear that the process of floral development in trigrain wheat is similar to that in cultivated common wheat, but the two additional gynoecia initiate after emergence of the first one (Wang et al 1987;Wang and Ding 1990). Recently, various lines of trigrain wheat with significant morphological variation were developed, and used for genetic studies (Wu et al 2000;Ma et al 2000Ma et al , 2006Peng 2003). The three grains trait was controlled by a single dominant gene, either with (Wu et al 2000), or without cytoplasmic effects (Zhi et al 2002;Peng 2003;Ma et al 2006), and by recessive genes (Shen et al 1992;Ma et al 2000).…”

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confidence: 99%

Genetic analysis and molecular markers associated with multi-gynoecia (Mg) gene in Trigrain whea

Wang¹,

Xu²,

Ji³

et al. 2009

Can. J. Plant Sci.

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Trigrain wheat normally produces up to three gynoecia in a single floret and forms three close-set grains. The gene conferring the multi grain phenotype was earlier designated Mg, the multiple gynoecia gene. Different genetic patterns controlling this trait have been reported. In the present work we studied the inheritance of the three grains trait and identified simple sequence repeats (SSR) markers linked to the Mg gene. Segregation analysis in the cross IGDB-TW (trigrain wheat)/Chinese Spring confirmed that a single dominant gene controlled the three grains trait. An allelism test showed that the same gene controlled the trigrain trait in line Trigrain-Yin 1. A total of 339 microsatellite markers were tested for polymorphism by bulked segregation analysis (BSA) in an F2 population. Six microsatellite markers, Xcfd233, Xgdm6, Xgdm87, Xgwm311, Xgwm349 and Xgwm539, on chromosome 2DL, were linked to Mg. Using the CS 2D deletion lines, Mg gene was localized to the distal region of chromosome 2DL. The microsatellite markers identified in this study have the potential for further mapping and map-based cloning of the gene.Key words: Simple sequence repeats, physical mapping, trigrain wheat

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confidence: 99%

Genetic analysis and molecular markers associated with multi-gynoecia (Mg) gene in Trigrain whea

Wang¹,

Xu²,

Ji³

et al. 2009

Can. J. Plant Sci.

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“…Pistillody phenomena are usually marked by partial or complete sex reversal and are associated with changes in their functions (Murai et al, 2002;2003). To study the mechanism for the homeotic transformation of stamens into pistils or pistil-like structures in wheat and identify the genes specifically or predominantly expressed in the pistillody wheat, we employed a new differential display RT-PCR technique, the ACP system (Hwang et al, 2003;Kim et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…The three-pistil (TP) mutant, which produces three pistils per floret, was screened by Peng (2003) and proven to be controlled by a single dominant gene, Pis1, located on chromosome 2D (Peng et al, 2004(Peng et al, , 2008Tiwari et al, 2011). Then, the HTS-1 plant was screened from the near-isogenic lines of TP wheat (CSTP) by Peng et al (2013).…”

Section: Introductionmentioning

confidence: 99%

Identification of differentially expressed genes in a pistillody common wheat mutant using an annealing control primer system

et al. 2015

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ABSTRACT. HTS-1 is a new kind of pistillody wheat. All or parts of its stamen are transformed into pistils or pistil-like structures, and it has more seed sets per floret than normal wheat under normal cultivation conditions. To investigate the expression divergence in this mutant, an annealing control primer system was used to identify differentially expressed genes (DEGs) in the young spikelets. As a result, three DEGs, including HDB2, HGF2, and HCG4, were detected, with variable expression in HTS-1 and the control. After further confirmation using real-time reverse transcription polymerase chain reaction analysis, these genes were overexpressed in HTS-1 wheat. NGF2 was identified in the double ridge to floret differentiation stages; HDB2 and HCG4 were identified in the stage of pistil and stamen-differentiating. Therefore, we inferred that the homeotic transformation of stamens into pistil-like structures occurred during the early stage of stamen development. Sequence alignment analysis revealed that HDB2 encodes a putative protein of 189 amino acids, (2015) with high homology to the DEAD-box ATP-dependent RNA helicase, and HCG4 was identical to the Chinese spring wheat cDNA clone predicted protein according to GenBank. However, NGF2 was not found to have significant similarity to any reported proteins, suggesting it is a new functional gene in wheat. The results suggest that HDB2, HCG4, and HGF2 are minor genes contributing to pistillody trait formation in HTS-1.

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“…Common wheat (Triticum aestivum L.) line three-pistil (TP) mutation is a novel mutant of flower development, which was selected by Peng (2003) from the "tri-grain" wheat cultivar. The TP mutant stably carries three pistils in a floret and shows three normal stamens, making it a valuable material in wheat flower development.…”

Section: Introductionmentioning

confidence: 99%

Codon Usage Bias of the Wheat Flower Development Gene WAG-2 and Other AGAMOUS Group Genes

Hu¹,

Wei²

2017

JAS

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Analyzing codon usage bias of WAG-2 gene in wheat three-pistil (TP) mutant may provide a basis for selecting the appropriate host expression systems to improve the expression of target genes. In the present study, we analyzed the codon bias of the complete coding sequence (CDS) of the WAG-2 gene in TP using Codon W program, and compared the results with AGAMOUS (AG) group genes of other plant species. Results showed that the WAG-2 gene in TP and other monocot AG group genes preferably used codons ending with G/C bases, but Arabidopsis thaliana, Nicotiana tabacum, and other dicot crops were biased toward the synonymous codons with A/T. The clustering results based on codon bias were consistent with those based on CDS of the AG group genes, indicating that the difference in codon preference of AG group genes sequences was closely associated with the genetic relationship of the species. The Euclidean distance coefficients of WAG-2 with A. thaliana and N. tabacum were 9.255 and 5.730, respectively, indicating that N. tabacum may be more suitable for the expression of WAG-2. There were 37 codons showing distinct usage differences between WAG-2 and genome of yeast, 23 between WAG-2 and Escherichia coli. Therefore, the E. coli was the superior protein expression system. These results may improve our understanding of codon usage bias and functional studies of WAG-2.

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A New Mutation in Wheat Producing Three Pistils in a Floret

Cited by 32 publications

References 3 publications

Genetic analysis and molecular markers associated with multi-gynoecia (Mg) gene in Trigrain whea

Genetic analysis and molecular markers associated with multi-gynoecia (Mg) gene in Trigrain whea

Identification of differentially expressed genes in a pistillody common wheat mutant using an annealing control primer system

Codon Usage Bias of the Wheat Flower Development Gene WAG-2 and Other AGAMOUS Group Genes

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