A New Paramyxovirus Isolated From Cynomolgus Monkeys

Characteristics of Murayama Virus in Various Cell Cultures and Laboratory Animals

“…In this study, several cell cultures were tested for their susceptibilities to the virus. The virus grew well in MK cells as mentioned before (Nishikawa et al, 1977a); as shown in Fig. 1, when the virus was inoculated at an input multiplicity of about 100 TCID50 per cell, the titers of the virus in the fluids increased exponentially from 6 hr after infection and reached a plateau in 10 hr, while the cell-associated virus was detected 4 hr after infection and gradually increased.…”

“…The previous serological survey of monkeys for Murayama virus performed in this laboratory disclosed that the Murayama virus-infected monkeys belonged to the group of the same shipment and were imported together with exotic pet birds by the same airplane (Nishikawa et al, 1977a). Therefore, a question arose whether this virus came from the bird.…”

“…We have already reported that Murayama virus grew well in chick embryos (Nishikawa et al, 1977a), the virus yield reaching about 108 EID50 per ml of the allantoic fluid. In this study, several cell cultures were tested for their susceptibilities to the virus.…”

“…From these biological properties and morphological features, Murayama virus was identified as a paramyxovirus. However, there was no antigenic relationship between Murayama virus and any other paramyxovirus available in this laboratory except Yucaipa and Bangore viruses, with which only one-way antigenic relationships were observed (Nishikawa, Sugiyama and Suzuki, 1977a). Recently, many paramyxoviruses have been isolated from animals, especially from domestic and wild birds, and their serological relationships have been studied (McFerran et al, 1974;Collings et al, 1975;Smit and Rondhui.s, 1976;Webster et al, 1976;Yamane et al, 1978;Shortridge and Alexander, 1978;Alexander et al, 1979).…”

Complete sequence of the genome of avian paramyxovirus type 2 (strain Yucaipa) and comparison with other paramyxoviruses

“…The F protein is 536 aa long with a predicted M r of 57.69 KDa. Among the available paramxyxovirus sequences, the F protein of Yucaipa was most closely related (80.6%) to that of Murayama virus (MrV), a monkey paramyxovirus that is antigenically related to Yucaipa virus and for which the F and HN sequences are available (Nishikawa et al, 1977, GenBank accession no. D13990).…”