2006
DOI: 10.1080/10242420500538274
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A new pathway for the synthesis of oligosaccharides by the use of non-Leloir glycosyltransferases

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Cited by 35 publications
(19 citation statements)
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“…These analogues have a glycosidic bond energy similar to that of sucrose and thus should drive synthetic reactions by non-Leloir glycosyl transferases. DManÁFru, D-GalÁFru, D-XylÁFru, and D-FucÁFru (all a-1,2-b-linked) were accepted by different LSs like the LS of B. subtilis (Seibel et al 2006a) and the LS of L. reuteri , thus presenting proof of principle for this strategy. The kinetic studies of the different substrates provided deeper insights into the LS mechanism (Seibel et al 2006c).…”
Section: Proof Of Concept: Sucrose Analogues As Novel Substrates For mentioning
confidence: 97%
“…These analogues have a glycosidic bond energy similar to that of sucrose and thus should drive synthetic reactions by non-Leloir glycosyl transferases. DManÁFru, D-GalÁFru, D-XylÁFru, and D-FucÁFru (all a-1,2-b-linked) were accepted by different LSs like the LS of B. subtilis (Seibel et al 2006a) and the LS of L. reuteri , thus presenting proof of principle for this strategy. The kinetic studies of the different substrates provided deeper insights into the LS mechanism (Seibel et al 2006c).…”
Section: Proof Of Concept: Sucrose Analogues As Novel Substrates For mentioning
confidence: 97%
“…The experiments were performed at pH 6.5 and 37 8C. SacB Bm , like other levansucrases [6,26], hydrolyzes sucrose and forms levan but also transfers fructose to acceptor molecules.…”
Section: Employment Of the New Model For Levansucrase Sacb Bm Catalysismentioning
confidence: 99%
“…The levansucrase SacB Bs was produced by E. coli BL21(DE3) cells containing the sacB Bs expression plasmid p24FTF11871 as previously described (Seibel et al [6]). The enzyme was purified using a 15 mL CM-Sepharose FF (GE Healthcare) column with a controlled pump mix system (Liquid Chromatography Controller LCC-500 Plus, LKB Pump P-500, Pharmacia) and a UV-detector (l = 280 nm, Biotech UV-MII, Pharmacia).…”
Section: B Subtilis Sacb Bsmentioning
confidence: 99%
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“…They have been evaluated as donor substrates for bacterial fructosyltransferases Seibel et al, 2006a). We have determined the kinetic parameters for wild-type levansucrase from B. subtilis and variant A5 with sucrose and sucrose analogues ( Table 1).…”
Section: Kinetic Parameters Of Levansucrasesacb Wild-type and Variantmentioning
confidence: 99%