A new perspective on the toxicity of arsenic-contaminated soil: Tandem mass tag proteomics and metabolomics in earthworms

Tang, Ronggui; Lan, Ping; Ding, Changfeng; Wang, Junsong; Zhang, Taolin; Wang, Xingxiang

doi:10.1016/j.jhazmat.2020.122825

Cited by 45 publications

(15 citation statements)

References 66 publications

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“…These results indicated that the open experimental system was not suitable for the volatile compounds. Moreover, there is no emphasis in the OECD and GB/T 31270.15-2014 guidelines for measurement of the chemical concentration, although a few studies pointed out the test concentrations in soil or within the exposed organisms [ 24 , 25 ]. In the present study, due to the short test period, the sealing of the test device, and the limited space, the closed soil microcosm could effectively reduce the loss of PAs, so as to ensure that the earthworms were exposed at the added concentrations, which could be affected at approximate real environmental concentrations.…”

Section: Resultsmentioning

confidence: 99%

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Acute Toxicity and Ecotoxicological Risk Assessment of Three Volatile Pesticide Additives on the Earthworm—Eisenia fetida

Wang

Zhang

Jingya

et al. 2021

IJERPH

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Pesticide adjuvants (PAs) are important components of pesticide. Nonetheless, limited information is available regarding their toxic effects on biota in terrestrial ecosystem. In the present study, the mortality, growth inhibition ratio, and avoidance behavior of Eisenia fetida were examined to investigate the ecotoxicological effects of toluene, xylene, and trichloroethylene and further their mixture. The 24 h median lethal concentration (24 h-LC50) of the three PAs were 300.23, 1190.45, and 5332.36 mg/kg, and the 48 h-LC50 values were 221.62, 962.89, and 4522.41 mg/kg, respectively. The mixture exhibited significant synergistic effect on the E. fetida. There was significant growth inhibition on E. fetida by the tested PAs. The avoidance threshold values of E. fetida for the tested PAs were 1100 mg/kg, 250 mg/kg, 5000 mg/kg, and 25% of the mixture, respectively. The results evaluated the toxic effects of the three PAs and their mixture on E. fetida, provided a basis for ecotoxicological risk assessment of PAs in the soil ecosystem.

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Section: Resultsmentioning

confidence: 99%

“…The selection rate, which is less than 10% in the avoidance test soil at 0 and 48 h, indicating that the presence of chemical substances cannot be excluded, is assessed as the avoidance threshold (AT) values [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Acute Toxicity and Ecotoxicological Risk Assessment of Three Volatile Pesticide Additives on the Earthworm—Eisenia fetida

Wang

Zhang

Jingya

et al. 2021

IJERPH

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“…This suggests that the long-term effects of low salinity exposure induced structural changes to the cell cytoskeleton to counter the effects of maintaining homeostasis in a hypotonic environment. Previous studies have identified upregulation of Profilin in response to osmotic stress in annelids [ 102 ], in addition to both oxidative and heavy-metal stress in M. galloprovincialis [ 103 , 104 ]. Osmotic stress is also associated with specific heat shock proteins, in particular small heat shock proteins (sHSPs), which are known to colocalise with the cell cytoskeleton when under osmotic stress [ 105 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience

Barrett

Thyrring

Harper

et al. 2022

Genes

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Increases in Arctic temperatures have accelerated melting of the Greenland icesheet, exposing intertidal organisms, such as the blue mussel Mytilus edulis, to high air temperatures and low salinities in summer. However, the interaction of these combined stressors is poorly described at the transcriptional level. Comparing expression profiles of M. edulis from experimentally warmed (30 °C and 33 °C) animals kept at control (23‰) and low salinities (15‰) revealed a significant lack of enrichment for Gene Ontology terms (GO), indicating that similar processes were active under all conditions. However, there was a progressive increase in the abundance of upregulated genes as each stressor was applied, with synergistic increases at 33 °C and 15‰, suggesting combined stressors push the animal towards their tolerance thresholds. Further analyses comparing the effects of salinity alone (23‰, 15‰ and 5‰) showed high expression of stress and osmoregulatory marker genes at the lowest salinity, implying that the cell is carrying out intracellular osmoregulation to maintain the cytosol as hyperosmotic. Identification of aquaporins and vacuolar-type ATPase transcripts suggested the cell may use fluid-filled cavities to excrete excess intracellular water, as previously identified in embryonic freshwater mussels. These results indicate that M. edulis has considerable resilience to heat stress and highly efficient mechanisms to acclimatise to lowered salinity in a changing world.

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“…Arsenic (As), a metalloid element, is widely distributed in the soil environment and As contamination is commonplace (Kwon et al, 2012). As contamination in soil may cause toxicity in plants and animals (Pendergrass and Butcher, 2006;Wang et al, 2018;Tang et al, 2020). Arsenic content in soils varies (3.5-935 mg As kg soil -1 ) in different reports (Huang et al, 2006;Liu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Proteomics mainly studies the functional proteins in the cells, tissues or organisms (Wang et al, 2010b;Tang et al, 2020). Using proteomics analysis, we can get direct information of the samples at protein level (Naaby-Hansen et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Effect of arsenite on the proteome of earthworms Eisenia fetida

Wang

Geng

et al. 2022

Soil Ecol. Lett.

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Arsenic (As) is broadly distributed due to natural and anthropogenic sources, and it is toxic to organisms. This study aimed to investigate the proteomic response in earthworm exposed to As 3+ . Earthworms were exposed to As 3+ in soil at 5-80 mg kg -1 , and samples were collected after 60 days exposure. Two-dimensional electrophoresis (2-DE) was used to separate the proteins in earthworm homogenate, then differentially expressed proteins (DEPs) were identified using MALDI-TOF/TOF-MS analysis. After 2-DE, 36 DEPs were found and 24 of them were successfully identified. 79.2% of DEPs were upregulated compared to the control group. The maximum fold change reached 53.8 in spot 3108 in the 80 mg kg -1 As group. Two proteins were not found in the control group but found in the As treated groups. Proteins were grouped into metabolism, signal transduction, stress-related, transport, regulation, and predicted/hypothetical protein categories based on their function. The protein-protein interaction between the DEPs indicated that serum albumin (ALB) is very important, related to 6 other proteins. Proteins were then verified by western blot, the results were in agreement with the proteomic analyses. The identification of induced or repressed proteins because of As 3+ in earthworms is helpful to explore the underlying mechanisms of soil arsenic ecotoxicity.

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A new perspective on the toxicity of arsenic-contaminated soil: Tandem mass tag proteomics and metabolomics in earthworms

Cited by 45 publications

References 66 publications

Acute Toxicity and Ecotoxicological Risk Assessment of Three Volatile Pesticide Additives on the Earthworm—Eisenia fetida

Acute Toxicity and Ecotoxicological Risk Assessment of Three Volatile Pesticide Additives on the Earthworm—Eisenia fetida

Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels (Mytilus edulis): The Limits of Resilience

Effect of arsenite on the proteome of earthworms Eisenia fetida

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