A new phospholipase a2 isoform isolated from Bothrops neuwiedii (yarará chica) venom with novel kinetic and chromatographic properties

Coupling Reaction and Properties of Poly(ethylene glycol)-linked Phospholipases A2

“…Despite the fact that these proteins have a very similar MW it was observed that these enzymes show some interaction with the matrix of gel permeation chromatography columns, as previously reported. 16) However, the treatment of the enzymes with MPEG did not change their chromatographic mobility either on Superdex-75 or Mono Q columns, which rules out an unspeciˆc binding of PEG to the enzymes. Approximately 95-100z of P-1 PLA2 from B. neuwiedii ( Fig.…”

“…15,16) P-1 PLA2 has a molecular mass (M) of 15 kDa with 8 Lys in its primary sequence, whereas P-3 PLA2 has a M of 15.2 kDa with 6 Lys. 15,16) N. naja naja acidic PLA2 (14 kDa, 6 Lys) was puriˆed from cobra venom as described. 14,17) Chromatography.…”

“…This modiˆcation was done essentially as described. 16,19) Brie‰y, BPB was added to the sample to give aˆnal concentration of 1×10 "4 M, and the mixture was incubated at room temperature during 24 h. BPB-modiˆed PLA2 was separated from free BPB by gelˆltration in a PD-10 columm.…”

A Review on Snake Venom: An Unrevealed Medicine for Human Ailments: Great Scope for Pharmaceutical Research

“…Vicharchika, Dadru, Kitibh (skin diseases) (External application) 23 Leaves, bark, and root of shamyak and snake venom macerated with buttermilk…”

“…22 Phospholipase A2 was isolated from Bothrops newweidii venom which produced cytotoxic activity on B16 F10 melanoma cell. 23 It is observed that Indian cobra Naja naja venom was cytotoxic to Ehrlich ascites tumor cells. 24 Cytotoxic P4 was isolated from Naja nigricollis venom, which produced cytotoxic effect on Wehi-B leukemia cell.…”