A new primer-pair for sex identification of larks and wagtails

Chu, Hui; Guo, Xinyi; Zeng, Yan; Zou, Xiaoyan; Guo, Sheng‐Ping; Zhao, Liang

doi:10.1007/s12686-014-0298-1

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…We collected blood from the brachial vein, from which we subsequently extracted genomic DNA with a Gentra Puregene Blood Kit (Qiagen, Hilden, Germany) following the manufacturer's protocol. We confirmed the sex of the individual using PCR amplification (Chu et al 2015). After running the sample on a 1% agarose gel to confirm the presence of high molecular weight DNA, we sent the extraction to the Cornell Weil Medical School (New York, USA), where they generated a 180 bp fragment library, a 3 kb mate-pair library and a 8 kb mate-pair library.…”

Section: Sample Collection Dna Extraction and Sequencingmentioning

confidence: 99%

De Novo Assembly of a High-Quality Reference Genome for the Horned Lark (Eremophila alpestris)

Mason

Pulgarín

Cadena

et al. 2020

G3 Genes|Genomes|Genetics

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The Horned Lark (Eremophila alpestris) is a small songbird that exhibits remarkable geographic variation in appearance and habitat across an expansive distribution. While E. alpestris has been the focus of many ecological and evolutionary studies, we still lack a highly contiguous genome assembly for the Horned Lark and related taxa (Alaudidae). Here, we present CLO_EAlp_1.0, a highly contiguous assembly for E. alpestris generated from a blood sample of a wild, male bird captured in the Altiplano Cundiboyacense of Colombia. By combining short-insert and mate-pair libraries with the ALLPATHS-LG genome assembly pipeline, we generated a 1.04 Gb assembly comprised of 2713 scaffolds, with a largest scaffold size of 31.81 Mb, a scaffold N50 of 9.42 Mb, and a scaffold L50 of 30. These scaffolds were assembled from 23685 contigs, with a largest contig size of 1.69 Mb, a contig N50 of 193.81 kb, and a contig L50 of 1429. Our assembly pipeline also produced a single mitochondrial DNA contig of 14.00 kb. After polishing the genome, we identified 94.5% of single-copy gene orthologs from an Aves data set and 97.7% of single-copy gene orthologs from a vertebrata data set, which further demonstrates the high quality of our assembly. We anticipate that this genomic resource will be useful to the broader ornithological community and those interested in studying the evolutionary history and ecological interactions of larks, which comprise a widespread, yet understudied lineage of songbirds.

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Section: Sample Collection Dna Extraction and Sequencingmentioning

confidence: 99%

De Novo Assembly of a High-Quality Reference Genome for the Horned Lark (Eremophila alpestris)

Mason

Pulgarín

Cadena

et al. 2020

G3 Genes|Genomes|Genetics

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“…PCR was used to amplify part of the CHD gene using the primers IntP2/IntP8 in the laboratory (Chu et al . 2015).…”

Section: Methodsmentioning

confidence: 99%

“…For these birds, we drew a blood sample from the vein in the wing and stored this in anhydrous alcohol. PCR was used to amplify part of the CHD gene using the primers IntP2/IntP8 in the laboratory (Chu et al 2015).…”

Section: Data Collectionmentioning

confidence: 99%

The signalling function of plumage colour traits in the Chestnut Thrush (Turdus rubrocanus)

Lou

Chen

Møller

et al. 2023

Ibis

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Melanin is a common pigment in birds, and several studies have shown that melanin‐based plumage coloration could signal individual body condition, social status and personality traits. However, most of these studies have focused on males, and little is known about the role of plumage coloration in females. Here, we investigated whether body condition and personality traits (activity and exploratory behaviour) were associated with plumage coloration in wild populations of the Chestnut Thrush Turdus rubrocanus, an apparently monochromatic species. We also tested whether the signals of plumage colour traits differed between sexes. In this study, we found that females had better body condition compared with males. Females with lower chroma and brightness of their black wings had better body condition, but no plumage colour measures reflected male body condition. Males with a lower red chroma of their brown back plumage were less active, and females with lower red chroma of the back plumage were more active. However, plumage coloration did not explain variation in exploratory behaviour. Our findings suggest that plumage colour traits may act as sex‐specific signals in Chestnut Thrushes and these traits may play important roles in sexual or conspecific communication in the wild.

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Using wing length for sexing Eurasian Skylark Alauda arvensis: examples from a British population

Hughes

Hopwood²,

Dolan³

et al. 2022

Ringing & Migration

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A new primer-pair for sex identification of larks and wagtails

Cited by 5 publications

References 18 publications

De Novo Assembly of a High-Quality Reference Genome for the Horned Lark (Eremophila alpestris)

De Novo Assembly of a High-Quality Reference Genome for the Horned Lark (Eremophila alpestris)

The signalling function of plumage colour traits in the Chestnut Thrush (Turdus rubrocanus)

Using wing length for sexing Eurasian Skylark Alauda arvensis: examples from a British population

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