A new printable and durable N,O-carboxymethyl chitosan–Ca<sup>2+</sup>–polyphosphate complex with morphogenetic activity

Müller, Wernér E.G.; Tolba, Emad; Schröder, Heinz C.; Neufurth, Meik; Wang, Shunfeng; Link, Thorben; Al‐Nawas, Bilal; Wang, Xiaohong

doi:10.1039/c4tb01586j

Cited by 72 publications

(77 citation statements)

References 46 publications

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“…To date, hydrogels based on natural biopolymers, such as alginate 57, 58 , gelatin 47, 54, 57 , collagen 60-62 , fibrin 62 , hyaluronic acid 55 , chitosan 63 , and agarose 45 , as well as many synthetic polymers like poly (ethylene glycol) (PEG) 64, 65 and pluronics 46 , have been demonstrated to fulfill some essential requirements for use as bioinks. These bioinks may not only provide the basis as sacrificial/constructive scaffolds, but can also maintain the viability and promote the activity of bioprinted living cells.…”

Section: Selection Of Bioinksmentioning

confidence: 99%

3D Bioprinting for Tissue and Organ Fabrication

et al. 2016

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The field of regenerative medicine has progressed tremendously over the past few decades in its ability to fabricate functional tissue substitutes. Conventional approaches based on scaffolding and microengineering are limited in their capacity of producing tissue constructs with precise biomimetic properties. Three-dimensional (3D) bioprinting technology, on the other hand, promises to bridge the divergence between artificially engineered tissue constructs and native tissues. In a sense, 3D bioprinting offers unprecedented versatility to co-deliver cells and biomaterials with precise control over their compositions, spatial distributions, and architectural accuracy, therefore achieving detailed or even personalized recapitulation of the fine shape, structure, and architecture of target tissues and organs. Here we briefly describe recent progresses of 3D bioprinting technology and associated bioinks suitable for the printing process. We then focus on the applications of this technology in fabrication of biomimetic constructs of several representative tissues and organs, including blood vessel, heart, liver, and cartilage. We finally conclude with future challenges in 3D bioprinting as well as potential solutions for further development.

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Section: Selection Of Bioinksmentioning

confidence: 99%

3D Bioprinting for Tissue and Organ Fabrication

et al. 2016

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“…[17]; reviewed in: refs. [26] The polyP nanoparticles can also be fabricated in hybrid forms, e.g., with N,O-carboxymethyl chitosan [27] or with hyaluronic acid [8] ; the nanoparticles retain their biological function. They are suitable for fabrication of bioinspired scaffold materials in tissue engineering bone and cartilage.…”

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confidence: 99%

In Situ Polyphosphate Nanoparticle Formation in Hybrid Poly(vinyl alcohol)/Karaya Gum Hydrogels: A Porous Scaffold Inducing Infiltration of Mesenchymal Stem Cells

et al. 2018

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The preparation and characterization of a porous hybrid cryogel based on the two organic polymers, poly(vinyl alcohol) (PVA) and karaya gum (KG), into which polyphosphate (polyP) nanoparticles have been incorporated, are described. The PVA/KG cryogel is prepared by intermolecular cross‐linking of PVA via freeze‐thawing and Ca2+‐mediated ionic gelation of KG to form stable salt bridges. The incorporation of polyP as amorphous nanoparticles with Ca2+ ions (Ca‐polyP‐NP) is achieved using an in situ approach. The polyP constituent does not significantly affect the viscoelastic properties of the PVA/KG cryogel that are comparable to natural soft tissue. The exposure of the Ca‐polyP‐NP within the cryogel to medium/serum allows the formation of a biologically active polyP coacervate/protein matrix that stimulates the growth of human mesenchymal stem cells in vitro and provides the cells a suitable matrix for infiltration superior to the polyP‐free cryogel. In vivo biocompatibility studies in rats reveal that already two to four weeks after implantation into muscle, the implant regions containing the polyP‐KG/PVA material become replaced by initial granulation tissue, whereas the controls are free of any cells. It is proposed that the polyP‐KG/PVA cryogel has the potential to become a promising implant material for soft tissue engineering/repair.

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“…Initially, those particles have been prepared with Ca 2+ as the counterion to the phosphate groups within the polyP. Ca 2+ was found also to self-organise and harden the gel-like polyP after bioprinting (Müller et al, 2015c). Since the aim of the present study is to outline a strategy for the injection of polyP-containing microparticles into the synovial fluid, amorphous polyP microparticles have been prepared with Mg 2+ as counterions (Mg-polyP).…”

Section: Introductionmentioning

confidence: 99%

Artificial cartilage bio-matrix formed of hyaluronic acid and Mg2+-polyphosphate

Wang¹,

Ackermann²,

Tolba³

et al. 2016

eCM

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Here we show that inorganic polyphosphate (polyP), a polyanionic metabolic regulator consisting of multiple phosphate residues linked by energy-rich phosphoanhydride bonds, is present in the synovial fluid. In a biomimetic approach, to enhance cartilage synthesis and regeneration, we prepared amorphous polyP microparticles with Mg 2+ as counterions. The particles were characterised by X-ray diffraction (XRD), energy-dispersive X-ray (EDX) and Fourier transformed infrared spectroscopic (FTIR) analyses. Similar particles were obtained after addition of Mg 2+ ions to a solution containing hyaluronic acid, as a major component of the synovial fluid, and soluble NapolyP. The viscous paste-like material formed, composed of globular microparticles with diameter of 400 nm, strongly promoted the adhesion of chondrocytes and caused a significant upregulation of the expression of the genes encoding collagen type 3A1, as a marker for chondrocyte differentiation, and SOX9, a transcription factor that regulates chondrocyte differentiation and proliferation. The expression level of the collagen type 3A1 gene was also enhanced by exposure of chondrocytes to synovial fluid that was found to contain polyP with a size of about 80 phosphate residues. This stimulatory effect was abolished after pre-incubation of the synovial fluid with the polyP degrading alkaline phosphatase. We propose a strategy for treatment of joint dysfunctions caused by osteoarthritis based on the application of amorphous Mg 2+ -polyP microparticles that prevent calcium crystal formation in the synovial fluid using scavenging Ca -polyP to hyaluronic acid at the cartilage surface.

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A new printable and durable N,O-carboxymethyl chitosan–Ca²⁺–polyphosphate complex with morphogenetic activity

Abstract: In the absence of Ca2+ the polymers N,O-carboxymethyl chitosan, together with Na-polyphosphate and alginate, form random-coiled structures. Addition of Ca2+ transforms these polymers to durable implants.

Cited by 72 publications

References 46 publications

3D Bioprinting for Tissue and Organ Fabrication

3D Bioprinting for Tissue and Organ Fabrication

In Situ Polyphosphate Nanoparticle Formation in Hybrid Poly(vinyl alcohol)/Karaya Gum Hydrogels: A Porous Scaffold Inducing Infiltration of Mesenchymal Stem Cells

Artificial cartilage bio-matrix formed of hyaluronic acid and Mg2+-polyphosphate

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A new printable and durable N,O-carboxymethyl chitosan–Ca2+–polyphosphate complex with morphogenetic activity

Abstract: In the absence of Ca2+ the polymers N,O-carboxymethyl chitosan, together with Na-polyphosphate and alginate, form random-coiled structures. Addition of Ca2+ transforms these polymers to durable implants.

Cited by 72 publications

References 46 publications

3D Bioprinting for Tissue and Organ Fabrication

3D Bioprinting for Tissue and Organ Fabrication

In Situ Polyphosphate Nanoparticle Formation in Hybrid Poly(vinyl alcohol)/Karaya Gum Hydrogels: A Porous Scaffold Inducing Infiltration of Mesenchymal Stem Cells

Artificial cartilage bio-matrix formed of hyaluronic acid and Mg2+-polyphosphate

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A new printable and durable N,O-carboxymethyl chitosan–Ca²⁺–polyphosphate complex with morphogenetic activity