A New Rat Model of Cisplatin-induced Neuropathic Pain

Abstract

BackgroundChemotherapy-induced peripheral neuropathy is a major side effect of anti-cancer drugs, and our knowledge of its mechanisms is lacking. Several models for chemotherapy-induced neuropathy have been introduced. However, the outcomes of these models differ significantly among laboratories. Our object was to create a model of chemotherapy-induced neuropathy in rats with cancer.MethodsFemale Sprague-Dawley rats were used. Mammary rat metastasis tumor (MRMT-1) cells were implanted subcutaneously in rats. Chemotherapy-induced peripheral neuropathy was induced by injection of cisplatin once a day for four days. The responses to mechanical and thermal stimuli were examined using von Frey filaments, acetone, and radiant heat.ResultsCisplatin (2 mg/kg/day) produced mechanical allodynia, while it did not induce cold allodynia or thermal hyperalgesia. This dose of cisplatin could work successfully against cancer. Body weight loss was not observed in cisplatin-treated rats, nor were other abnormal behaviors noted in the same rats.ConclusionsRepeated injection of intraperitoneal cisplatin induced peripheral neuropathic pain in rats. Thus, this type of rat model has broad applicability in studies related to searching for the mechanism of cisplatin-induced mechanical allodynia and agents for the treatment of neuropathic pain.

N-acetylcysteine versus progesterone on the cisplatin-induced peripheral neurotoxicity

“…Cisplatin-induced sciatic nerve damage in the rat model has been proven in many studies [34]. Cisplatin has a molecular affinity for the peripheral nerves as they lack the vascular and lymphatic barriers, which make them more susceptible to the precipitation of the cisplatin debris [25,41].…”

SIRT2 protects peripheral neurons from cisplatin-induced injury by enhancing nucleotide excision repair

“…Reported literature on the effect of cisplatin on thermal sensitivity currently shows a discrepancy in murine models. Although one study saw no difference in thermal hyperalgesia on hot plate studies (46), others demonstrate enhanced sensitivity to noxious heat following cisplatin, more so than oxaliplatin, treatment (43,47,48). Likewise, mechanical hyperalgesia has been documented in mice as measured by decreased pressure tolerance on von Frey filament tests.…”

Mental Neuropathy as Initial Manifestation of Metastatic Breast Cancer: A Case Report and Review of the Literature

“…Thus, patients with breast cancer receiving this drug class often suffer from neuropathy caused by medication side effects during treatment. However, this side effect can affect the patient for many years and sometimes indefinitely [35][36][37][38][39]. In our reported patient, mental neuropathy cannot be attributed to chemotherapy drugs, because the neuropathy had developed recently, but chemotherapy had finished three years earlier, and the patient also had no signs of sensory impairment during treatment.…”