A New Rice Seedling Test for Gibberellins, 'Microdrop Method', and its Use for Testing Extracts of Rice and Morning Glory

Murakami, Yutaka

doi:10.15281/jplantres1887.81.33

Cited by 189 publications

(98 citation statements)

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“…4), the specific radioactivity was 33 1 Supported by National Research Council of Canada Grants A-5727 to D. M. R., A-6467 to T. A. T., and A-2585 to R. P. P. 2 methyl ester can be expected to involve mainly cis addition of hydrogen f8 to the molecule at positions C-2 and C-3 as was noted for hydrogenation of GA3 which gave GA, (18 (11). The Tan-ginbozu dwarf rice microdrop assay (13) and lettuce hypocotyl assay (8) were used. Rice seedlings, 10/extract, were treated with 0.5 ,ul of serial dilutions in 95% ethanol and the second leaf sheath measured at hr 48.…”

Section: Methodsmentioning

confidence: 95%

Metabolism of [³H]Gibberellin A₂₀ in Light- and Dark-grown Tobacco Callus Cultures

Lance

Durley²,

Reid³

et al. 1976

Plant Physiol.

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The growth of tobacco callus in culture (previously shown to contain gibberelin [GAJ-like substances), and its abilit to metabolize 13HI-GA20 were examined. Growth rates, in the presence and absence of exogenously applied GA, were examined in light and dark conditions. Dark-grown callus grew at a much faster rate than light-grown and [3HIGA2o was metabolized much more rapidly in darkness than in light.[3HIGA, was identified by combined gas-liquid chromatography/mass spectrometry as a major product of 13H1GA2o, and was found to be a more potent promoter of tobacco callus growth than GA20.In an earlier paper (11), evidence was presented showing that tobacco callus contains endogenous gibberellins (GAs), the levels of which varied with cultural treatments such as light or darkness or the concentration of cytokinin in the medium. On this and other evidence, it was postulated that endogenous GAs might play a role in controlling growth of tobacco callus cells.Variations in endogenous GA levels could arise through modifications of rate of biosynthesis and/or utilization (i.e. metabolism). Extraction of plant tissue at any one point in time or even at a number of times provides information only on GA pool size, and will often reveal little about over-all GA metabolism. This paper describes an investigation of the metabolism of [3H]GA20

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Section: Methodsmentioning

confidence: 95%

Metabolism of [³H]Gibberellin A₂₀ in Light- and Dark-grown Tobacco Callus Cultures

Lance

Durley²,

Reid³

et al. 1976

Plant Physiol.

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“…Six oilseed rape (Brassica napus, annual Canola variety 'Westar') seeds were planted January 28, 1986 (10,12), prior to step-elution sio2 partition chromatography (2, 13). Fractions were bioassayed in serial dilution with the Tan-ginbozu dwarf rice microdrop assay (6,12).…”

Section: Methodsmentioning

confidence: 99%

Identification of Endogenous Gibberellins from Oilseed Rape

Rood¹,

Pearce²,

Pharis³

1987

Plant Physiol.

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Oilseed rape (Brasoica napia, cola variety 'Westar') plants were grown in greenhouse conditions and shoots were harvested during the final stages of shoot elatio.Leaves and ture pods were removed and the rewmin ng stem tsue was exracted and puified. The extract was chronatographed on sequential, step-eluted silica gel parit and reverse-phae Cl, HPLC columns, and gibberelin (GA)-like substances were detected using the 'Tan-ginbozu' dwarf rice mirodrop assay. Purified frctions showing GA-like activity were analyzed by capiUary gas chromatography-mass spectrometry (GC-MS) and GC-selected ion monitoring (GC-SIM). Gibberellins A,, A3, and iso-A3 were identified by full spectrum GC-MS with GA, being the most abundant GA in the stem tissue. Gibberellins A,g and A2e were identified by GC-SIM and are logical precursors of the GA,.

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“…This fraction was subjected to preparative HPLC as noted in Reeve et al (20). Seventy 10-ml fractions were collected and aliquots were assayed for radioactivity by liquid scintillation counting, and GA-like activity in the barley aleurone (50-fold dilution), Tan-ginbozu dwarf rice microdrop (50-fold dilution), and lettuce hypocotyl (30-fold dilution) bioassays (5,16,17). Fractions containing GAlike activity were analyzed further by analytical HPLC and/or GC-MS.…”

Section: Methodsmentioning

confidence: 99%

Identification of Endogenous Gibberellins from Salix pentandra

Davies¹,

Jensen²,

Junttila³

et al. 1985

Plant Physiol.

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A New Rice Seedling Test for Gibberellins, 'Microdrop Method', and its Use for Testing Extracts of Rice and Morning Glory

Cited by 189 publications

References 0 publications

Metabolism of [³H]Gibberellin A₂₀ in Light- and Dark-grown Tobacco Callus Cultures

Metabolism of [³H]Gibberellin A₂₀ in Light- and Dark-grown Tobacco Callus Cultures

Identification of Endogenous Gibberellins from Oilseed Rape

Identification of Endogenous Gibberellins from Salix pentandra

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A New Rice Seedling Test for Gibberellins, 'Microdrop Method', and its Use for Testing Extracts of Rice and Morning Glory

Cited by 189 publications

References 0 publications

Metabolism of [3H]Gibberellin A20 in Light- and Dark-grown Tobacco Callus Cultures

Metabolism of [3H]Gibberellin A20 in Light- and Dark-grown Tobacco Callus Cultures

Identification of Endogenous Gibberellins from Oilseed Rape

Identification of Endogenous Gibberellins from Salix pentandra

Contact Info

Product

Resources

About

Metabolism of [³H]Gibberellin A₂₀ in Light- and Dark-grown Tobacco Callus Cultures

Metabolism of [³H]Gibberellin A₂₀ in Light- and Dark-grown Tobacco Callus Cultures