A New RP-HPLC Method for Determination of Roflumilast in Bulk and Pharmaceutical Dosage Forms

Abstract

Background Rofumilast drug estimation was done earlier by spectroscopic methods. Only few chromato graphic studies were reported. Objective Development and validation of RP-HPLC method for the drug Roflumilast by using various chromatographic parameters. Methodology RP-HPLC method was developed with 1 mlmin as flow rate and 10 microl was set as volume of injection for about 15 minutes of run time. Various parameters like accuracy linearity precision were validated for the method as per ICH guidelines.Results UV detection for maximum wavelength at 250 nm with mobile phase of 3070 ratio of Trifluroacetic acid pH 3.2 Acetonitrile showed the retention time of 2.317 min. Conclusion The proposed RP- HPLC method was found to be rapid simple precise accurate and economic forestimation of roflumilast in bulk and pharmaceutical formulations.