A new scheme for serotyping group-G streptococci

Abstract

Summary. Ten antisera, prepared against nine seiected strains of group-G streptococci and a strain of group-A streptococci of M-type 12, were used to serotype 102 isolates of group-G streptococci by means of precipitation reactions between the sera and hot-acid extracts of the streptococci. Fifty-six (54.973 of the streptococci could be typed; eight serotypes were identified, of which type VIII was the most common (18.6%). The MI2 and the R28 antigens, previously recorded in group-G streptococci, were not detected. The type antigens were trypsin sensitive and resembled the M antigens of group-A streptococci.

Group G Streptococcal Bacteremia in Jerusalem

“…Fifty-six isolates (54%) could be serologically typed into eight serotypes (38). Of 128 isolates, 40 (31%) could be serotyped with six antisera (39).…”

M or M-like protein gene polymorphisms in human group G streptococci

“…Within the last few years, molecular genetic techniques became important methods to identify and characterize emm or emm-like genes of GAS (9,15,18,20,21,34,35). Thus far, however, identification of M or M-like proteins in GGS is primarily based on functional and serological analysis (7,25,28,31), and only a few GGS emm or emmL genes were studied by molecular means (3,5,11,45). Therefore, the development of the PCR assay established in this study that allows probably universal amplification of emm or emmL genes from these bacteria could certainly accelerate their investigation.…”