A new selective medium for the isolation of glucose non-fermenting bifidobacteria from hen caeca

Rada, V.; Petr, Jan

doi:10.1016/s0167-7012(00)00205-0

Cited by 124 publications

(101 citation statements)

References 14 publications

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“…Isolations were performed using modified TPY agar (Sharlau, Barcelona, Spain) supplemented with mupirocin (100 g/l) and glacial acetic acid (1 ml/l) according Rada and Petr (2000). Bifidobacterium isolates were identified by the following criteria: (i) they are Gram-positive pleomorphic rods, (ii) they show fructose-6-phosphate phosphoketolase activity (F6PPK) as determined by the method described by Orban and Patterson (2002), (iii) they have a positive reaction with genus-specific primers (Kok et al, 1996), (iv) they have a positive reaction with fluorescence-labelled probe (a kit for Bifidobacterium sp., RiboTechnologies, Groningen, The Netherlands).…”

Section: Bacteria Isolation and Identificationmentioning

confidence: 99%

“…Appropriate dilutions were transferred to sterile Petri dishes, which were immediately filled with the media for bifidobacteria (TPY agar, Sharlau, Spain) supplemented with mupirocin (100 mg/l) and glacial acetic acid (1 ml/l) according to Rada and Petr (2000), lactobacilli (Rogosa agar, Oxoid) and anaerobes (Wilkins-Chalgren, Oxoid). Bifidobacteria and anaerobic bacteria were incubated in anaerobic jars (Anaerobic Plus System, Oxoid) at 37°C for 72 h. Lactobacilli were incubated aerobically at 37°C for 48 h. Petri dishes with TBX agar (Oxoid) for E. coli and Slanetz-Bartley (Oxoid) for enterococci were inoculated with 0.1 ml of an appropriate dilution and spread using sterile glass rods.…”

Section: Sampling Bacteria Enumeration and Detection Of Administerementioning

confidence: 99%

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Selection of probiotic bifidobacteria for lambs

Vlková¹,

Grmanová²,

Rada³

et al. 2009

CZECH J ANIM SCI

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Twenty-six bifidobacteria were isolated from faecal samples of lambs. The isolates were identified, functional properties (survival ability at low pH and bile conditions) and antimicrobial activities against potential pathogens were determined. From the isolates with suitable properties (13 strains) rifampicinresistant mutants were prepared by gradient plate techniques. This property enabled us to differentiate the administered organism from wild strains because resistance to rifampicin is rare among bifidobacteria. Rifampicin-resistant bifidobacteria (RRBifs) were administered to 3-days-old lambs in two trials. In the first trial the strain B. ruminantium L29 was applied to 3 lambs and was detected in faecal samples at high counts (6 log CFU/g on average) for one week. In the second trial 3 lambs received a "cocktail" of 12 strains and RRBifs survived in the intestinal tract at counts of about 6 log CFU/g for 25 days. The control group without probiotic treatment consisted of 6 animals. In both treated groups RRBifs dominated among bifidobacteria after their administration. Total bifidobacterial counts (5.64-7.32 log CFU/g) were significantly higher (P < 0.05) in treated groups compared to 2.31-2.85 log CFU/g detected in the control group during the first month of lamb life. Lactobacilli counts were also significantly higher (P < 0.05) in treated groups compared to the control. The administered bifidobacteria did not affect any other monitored bacterial groups. On the basis of in vitro test results, suitable probiotic bifidobacterial strains for lambs were chosen. Some of them survived for 30 days in the gastrointestinal tract of treated lambs, but no tested strain was able to colonise the lamb's tract permanently. The administration of bifidobacterial "cocktail" and consequent identification of the best survived strain seems to be an effective method for selection of potential probiotics.

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Section: Bacteria Isolation and Identificationmentioning

confidence: 99%

Section: Sampling Bacteria Enumeration and Detection Of Administerementioning

confidence: 99%

Selection of probiotic bifidobacteria for lambs

Vlková¹,

Grmanová²,

Rada³

et al. 2009

CZECH J ANIM SCI

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“…Samples of fresh rectal swabs from common marmosets were serially diluted with peptone water (Merck) supplemented with cysteine hydrochloride (0.5 g l 21 ); aliquots of each dilution were inoculated onto TPY agar supplemented with mupirocine (100 mg l 21 ; Applichem), which is a selective agent for bifidobacteria (Rada & Petr, 2000). In each subject, we observed cells of a bacterium with a novel and unusual morphology, resembling a coiled snake.…”

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confidence: 99%

Bifidobacterium aesculapii sp. nov., from the faeces of the baby common marmoset (Callithrix jacchus)

Modesto

Michelini

Stefanini

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Bifidobacterium aesculapii sp. nov., from the faeces of the baby common marmoset (Callithrix jacchus) Six Gram-positive-staining, microaerophilic, non-spore-forming, fructose-6-phosphate phosphoketolase-positive bacterial strains with a peculiar morphology were isolated from faecal samples of baby common marmosets (Callithrix jacchus). Cells of these strains showed a morphology not reported previously for a bifidobacterial species, which resembled a coiled snake, always coiled or ring shaped or forming a 'Y' shape. Strains MRM 3/1 T and MRM 4/2 were chosen as representative strains and characterized further. The bacteria utilized a wide range of carbohydrates and produced urease. Glucose was fermented to acetate and lactate. Strain MRM 3/1 T showed a peptidoglycan type unique among members of the genus Bifidobacterium. The DNA base composition was 64.7 mol% G+C. Almost-complete 16S rRNA, hsp60, clpC and rpoB gene sequences were obtained and phylogenetic relationships were determined. Comparative analysis of 16S rRNA gene sequences showed that strains MRM 3/1 T and MRM 4/2 had the highest similarities to Bifidobacterium scardovii DSM 13734 T (94.6 %) and Bifidobacterium stellenboschense DSM 23968 T (94.5 %). Analysis of hsp60 showed that both strains were closely related to B. stellenboschense DSM 23968 T (97.5 % similarity); however, despite this high degree of similarity, our isolates could be distinguished from B. stellenboschense DSM 23968 T by low levels of DNA-DNA relatedness (30.4 % with MRM 3/1 T ). Strains MRM 3/1 T and MRM 4/2 were located in an actinobacterial cluster and were more closely related to the genus Bifidobacterium than to other genera in the family Bifidobacteriaceae. On the basis of these results, strains MRM 3/1 T and MRM 4/2 represent a novel species within the genus Bifidobacterium, for which the name Bifidobacterium aesculapii sp. nov. is proposed; the type strain is MRM 3/1 T (5DSM 26737 T 5JCM 18761 T ).

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“…Wilkins-Chalgren agar (50 g/l; Oxoid) supplemented with soya peptone (5 g/l; Oxoid), l-cystein (0.5 g/l; Sigma-Aldrich, USA), and Tween 80 (1 ml/l; Sigma-Aldrich) was used for enumeration of total anaerobic bacteria. Bifidobacteria were enumerated on the same agar as total anaerobes with the addition of glacial acetic acid (1 ml/l) and the antibiotic mupirocin (100 mg/l; Oxoid), according to a method reported by Rada and Petr (2000). These plates were incubated in anaerobic jars (Anaerobic Plus System, Oxoid) at 37°C for 72 h. To enumerate lactobacilli, Rogosa agar (82 g/l; Oxoid) adjusted to pH 5.4 ± 0.2 with glacial acetic acid was used.…”

Section: Methodsmentioning

confidence: 99%

Effect of dietary lupin (Lupinus albus) on the gastrointestinal microbiota composition in broiler chickens and ducks

Geigerová¹,

Švejstil²,

Skřivanová³

et al. 2017

CZECH J ANIM SCI

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Geigerová M., Švejstil R., Skřivanová E., Straková E., Suchý P. (2017): Effect of dietary lupin (Lupinus albus) on the gastrointestinal microbiota composition in broiler chickens and ducks. Czech J. Anim. Sci., 62, 369-376.The purpose of the study was to evaluate the amount of raffinose-series oligosaccharides (RSO) in soybean meal (SBM), whole white lupin seed meal (WLM), sunflower meal (SFM), and rapeseed oil meal (ROM) and to determine whether partial or complete dietary WLM replacement affected the numbers of bacteria in selected groups in the microbiota of broiler chickens and ducks without inducing any weight loss. Total counts of anaerobes, lactobacilli, bifidobacteria, and Escherichia coli in caecal samples from both ducks and broiler chickens, as well as in a crop chyme, in broiler chickens, were determined. Live weights before slaughter were determined. Both broiler chickens and ducks were fed a control diet with SBM (L 0 ) or diet containing 50% or 100% WLM as a substitute for SBM (groups L 50 and L 100 , respectively). In comparison with SBM, WLM contained significantly higher amounts of RSO, and the amounts of oligosaccharides in SFM (1.73 ± 0.26 g/100 g) and ROM (1.79 ± 0.14 g/100 g) were negligible compared to those in WLM (8.26 ± 0.14 g/100 g) and SBM (6.96 ± 0.21 g/100 g). The inclusion of lupin in chicken diets did not significantly affect the monitored bacterial groups in crop chyme, but a complete replacement of SBM with WLM (L 100 group) in chicken diets significantly (P ≤ 0.05) increased the counts of lactobacilli in caecal samples. Partial (L 50 group) and complete (L 100 group) lupin supplementation in the duck diet significantly (P ≤ 0.05) increased counts of lactobacilli and bifidobacteria by at least one order of magnitude. E. coli counts in poultry were not affected by changes in diet. The results of our study indicate that partial dietary replacement of SBM with WLM did not significantly affect the live weight of broiler chickens and ducks, but that complete replacement of SBM with WLM may lead to weight loss.

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A new selective medium for the isolation of glucose non-fermenting bifidobacteria from hen caeca

Cited by 124 publications

References 14 publications

Selection of probiotic bifidobacteria for lambs

Selection of probiotic bifidobacteria for lambs

Bifidobacterium aesculapii sp. nov., from the faeces of the baby common marmoset (Callithrix jacchus)

Effect of dietary lupin (Lupinus albus) on the gastrointestinal microbiota composition in broiler chickens and ducks

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