A new sensitive short pentaplex (ShoP) PCR for typing of degraded DNA

Meißner, Christoph; Bruse, Petra; Mueller, Ernest; Oehmichen, M.

doi:10.1016/j.forsciint.2006.04.014

Cited by 17 publications

(12 citation statements)

References 35 publications

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“…The problem with which forensic scientists have recently been confronted involves the technical difficulty of analyzing degraded DNA samples. In recent years, several papers have demonstrated that short amplicon STR (miniSTR) represents one of the most useful tools for analyzing degraded DNA samples [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. On the other hand, given Y-STRs role as a characteristic feature of haploid paternal inheritance without recombination, analysis of Y-STR plays a major role in male personal identification, deficiency paternity cases including male relatives, and male profiling for sexual assaults involving male-female stain mixtures.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of miniY-STR multiplex PCR systems for extended 16 Y-STR loci

et al. 2007

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We developed three short amplicon Y-chromosomal short tandem repeat (miniY-STR) polymerase chain reaction multiplex systems for 16 Y-STR loci (DYS441, DYS446, DYS462, DYS481, DYS485, DYS495, DYS505, DYS510, DYS511, DYS549, DYS 575, DYS578, DYS593, DYS618, DYS638, and DYS643), using newly designed primer sets. In an assay of 238 Japanese males using the three miniY-STR systems, amplification product lengths ranged from 91 to 151 bp for all 16 Y-STR loci. We identified 212 different haplotypes among the 238 individuals, finding haplotype diversity and discrimination capacity of 0.9974 and 0.8908, respectively. An assay of degraded DNA samples using the three miniY-STR multiplex systems, including artificially degraded samples and degraded forensic casework samples, proved remarkably effective. In conclusion, analyses of miniY-STR multiplex systems will play an important role in forensic applications involving degraded DNA samples for which genotyping using only commercial kits is ill-suited.

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Section: Introductionmentioning

confidence: 99%

Evaluation of miniY-STR multiplex PCR systems for extended 16 Y-STR loci

et al. 2007

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“…Thus, the specimens pass through a chemical and physical ageing process which also causes a reduced survival rate in tardigrades after rehydration . For degraded template DNAs other pairs of primers for small amplicons or other methods for typing of degraded DNA should be used, which are common in the field of forensic research (Timken et al 2005;Meissner et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Comparison of different protocols for DNA preparation and PCR amplification of mitochondrial genes of tardigrades

Schill¹

2007

J Limnol

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“…German specimens for sequencing analysis were selected, choosing different alleles in the known vWF Pol K polymorphism [13][14][15][16][17][18][19]. In addition, some people from Asia and Africa and cell line DNA were included.…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we present vWF intron 40 sequencing results on 68 chromosomes and an appropriate allele STR nomenclature, which is in compliance with the ISFG recommendations [3]. Our survey regarding the intron 40 SNPs may assist primer selection for special purposes such as the creation of short amplicon PCR kits [13,27]. Furthermore, SNP studies can provide inferences of the population and ancestry to which an individual belong [28].…”

Section: Introductionmentioning

confidence: 99%

Complex variability of intron 40 of the von Willebrand factor (vWF) gene

et al. 2007

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Intron 40 of the von Willebrand factor (vWF) gene exhibits a highly variable region of about 0.65 kb, which contains 5 juxtaposed STRs. We sequenced 0.65 kb amplicons from 68 chromosomes and found 2 frequent indel polymorphisms and 5 SNPs. The 68 chromosomes investigated here presented a total of 47 different haplotypes. Regarding the SNP allele distribution in our sample, we arranged our results of the vWF intron 40 into a system of 3 haplotypes, i.e. haplotypes a, b and c. Our review may be valuable in further optimising vWF typing in forensic applications and in avoiding pitfalls. Further attempts to develop sophisticated techniques may soon enable haplotyping using autosomale STR clusters.

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A new sensitive short pentaplex (ShoP) PCR for typing of degraded DNA

Cited by 17 publications

References 35 publications

Evaluation of miniY-STR multiplex PCR systems for extended 16 Y-STR loci

Evaluation of miniY-STR multiplex PCR systems for extended 16 Y-STR loci

Comparison of different protocols for DNA preparation and PCR amplification of mitochondrial genes of tardigrades

Complex variability of intron 40 of the von Willebrand factor (vWF) gene

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