A new simple liquid chromatographic assay for gentamicin in presence of methylparaben and propylparaben

Abstract

Gentamicin sulfate is a potent broad spectrum aminoglycoside antibiotic which is used against Gram-positive and Gram-negative bacteria. A simple, isocratic HPLC method for separation, identification and determination of gentamicin and parabens (methylparaben and propylparaben) was developed and validated. To our knowledge there is no report about simultaneous determination of those three analytes in pharmaceutical products. The optimum chromatographic conditions were achieved on CN column with a mobile phase consisting of 0.15% triethylamine in 10 mM KH2PO4 aqueous solution (final pH 3.0 adjusted with H3PO4) and methanol in the ratio 70:30 (v/v), providing selective quantification of analytes within 5 min. The method was successfully validated according to ICH guidelines acceptance criteria in terms of selectivity, linearity, accuracy, precision and robustness. The linearity of the method was proved in defined concentrations ranges for gentamicin (0.32–1.04 mg mL−1), methylparaben (0.0072–0.0234 mg mL−1) and propylparaben (0.0008–0.0026 mg mL−1). Relative standard deviations calculated for all analytes in precision testing were <2% (analysis repeatability) and <3% (intermediate precision). Recovery values were between 98.87% and 101.67%. Chromatographic parameters are not significantly influenced by small variations of column temperature, pH and molarity of KH2PO4. Finally, the method was successfully applied for quantitative determination of gentamicin and parabens in commercially available solution for injection. Proposed HPLC method is found to be promising in terms of simplicity, analysis times and non-use of derivatization and ion-pair agents.

From Polymeric Nanoformulations to Polyphenols—Strategies for Enhancing the Efficacy and Drug Delivery of Gentamicin

“…Firstly, gentamicin is a complex mixture of five structurally distinct components (see Section 2). Secondly, gentamicin lacks chromophore groups, which means that it cannot absorb UV light and can be indirectly determined through chemical derivatization methods with o-phthaldialdehyde, phenyl isocyanate, 9-fluorenylmethyl chloroformate, 1-fluoro-2,4-dinitrobenzene, and others, which are timeconsuming and produce unstable derivatives [114,115]. Most of the literature on gentamicin release from PLGA and chitosan mentions derivatization with o-phthaldialdehyde or ninhydrin [11,30,63,[65][66][67].…”

“…One of the most popular is the more rapid and cost-effective UV/VIS method. Alternatively, the most reliable method is high-performance liquid chromatography (HPLC), which enables UV, fluorescence, electrochemical, or MS detection [114,115].…”

Anthropocentric perspective on climate variability: the destination of antibiotics in the Tigris river is not restricted

“…Gentamycin is one of the widest spectrum antibiotics used for the treatment of infectious diseases, is highly soluble in water and less soluble in organic solvents (Arhoutane et al 2019), and has an electrochemical activity due to the hydroxyl and amino groups. Ivkovic et al (2023) reported that evaporation, light scattering, and pulsed electrochemical detection methods were employed to detect gentamycin in water.…”

A new selective colorimetric method coupled with a high-resolution UV method for the consecutive quantification of three drugs in semi-solid preparations

“…Gentamycin (GEN) is an aminoglycoside bactericidal, Figure 1 , works by inhibiting the synthesis of protein in bacteria [ 3 ], it was quantified via spectrophotometry [ 19 , 20 , 21 , 22 , 23 , 24 ],spectrofluorimetric [ 25 , 26 ], HPLC [ 27 , 28 , 29 ], and TLC [ 30 ].…”