2009
DOI: 10.1016/j.cca.2009.09.002
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A new standardized absolute quantitative RT-PCR method for detection of tyrosinase mRNAs in melanoma patients: Technical and operative instructions

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Cited by 8 publications
(7 citation statements)
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“…In fact, although we have set up a robust assay for absolute quantification of TYR transcripts in blood [1], we did not find any traceable copy of this marker on overall individuals examined (patients and controls). Excluding a methodological trouble, since our standard tyrosinase curve reached high amplification performance (as shown by E value and Pearson correlation coefficient r obtained), we could only hypothesize that in our cohort of patients the level of this transcript should be correlated to the molecular phenotype of circulating melanoma cells.…”
Section: Discussioncontrasting
confidence: 65%
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“…In fact, although we have set up a robust assay for absolute quantification of TYR transcripts in blood [1], we did not find any traceable copy of this marker on overall individuals examined (patients and controls). Excluding a methodological trouble, since our standard tyrosinase curve reached high amplification performance (as shown by E value and Pearson correlation coefficient r obtained), we could only hypothesize that in our cohort of patients the level of this transcript should be correlated to the molecular phenotype of circulating melanoma cells.…”
Section: Discussioncontrasting
confidence: 65%
“…In the EU, the incidence of melanoma is annually 9 cases/100.000, while in Italy, a total of 3,143 newly diagnosed cases among males and 2,851 among females [2]. Unfortunately, despite the increasing availability of screening program for melanoma prevention, the sensitivity of both clinical and laboratory diagnostic methods is still low in terms of early discovering of the primary melanoma or of prediction of its metastatic spreading [1]. Currently, the major part of therapies are ineffective for treating metastatic disease, with an overall response rate of 20%-40% and a complete response rate (or cure rate) less than 5%.…”
Section: Introductionmentioning
confidence: 94%
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“…Total RNA was extracted from serum as described by Vendittelli et al (9). The quality and integrity of total RNA was evaluated using gel electrophoresis and spectrophotometric determination.…”
Section: Methodsmentioning
confidence: 99%
“…The molecular biology offers accurate and more sensitive routes to detect melanoma circulating cells in blood, representing a relevant prognostic tool for predicting metastatic spreading (Venditelli et al, 2009). Markers, such as tyrosinase (Smith et al, 1991), microphthalmia-associated transcription factor (Garraway et al, 2005), melanoma antigen recognized by T-cells-1 or Melan-A antibody (Koyanagi et al, 2005), have been used for diagnosis of metastatic melanoma cells, by reverse transcription-polymerase chain reaction (RT-PCR).…”
Section: Introductionmentioning
confidence: 99%