A New Storage Medium for an Avulsed Tooth

Abstract

Aim The purpose of this study is to determine the efficacy of egg white in maintaining the viability of human periodontal ligament (PDL) cells on avulsed teeth. Methods and Materials The experimental media were: egg white, milk, Hanks’ Balanced Salt Solution (HBSS) as the positive control, and tap water as the negative control. The storage times were 1, 2, 4, 8, and 12 hours. Extracted premolar teeth of healthy individuals were rinsed in the media. After trypsinization and subsequent treatment in collagenase, cell viability was determined using trypan blue staining. The two-way analysis of variance (ANOVA) statistical test was used to compare the results among different media. Results There was no difference in the cell viability between egg white and HBSS media, but there was a statistically significant difference between the viability of PDL cells in egg white medium in comparison with milk (P<0.05) and water (P<0.05). Conclusion Egg white could be suggested as a suitable storage medium. Its principle advantage is its availability. Citation Khademi AA, Saei S, Mohajeri MR, Mirkheshti N, Ghassami F, Torabi nia N, Alavi SA. A New Storage Medium for an Avulsed Tooth. J Contemp Dent Pract 2008 September; (9)6:025-032.

The Use of Green Tea Extract as a Storage Medium for the Avulsed Tooth

“…Especially, HBSS and GTE were most effective in preserving PDL cell viability for 24 hours because they could preserve the viability over 90%. The result of HBSS effectiveness is in agreement with other studies (8,21,32). Buttke and Trope (33) suggested that the storage of avulsed teeth in a medium containing one or more antioxidant components might increase the success rate of replantation.…”

“…Extraoral time and storage medium are the critical factors responsible for prognosis of avulsed tooth (8,20,21). The longer the exposure of avulsed tooth to dry storage, the worse the prognosis of explantation is.…”

“…Both physiological osmolality and pH are important factors in preserving the viability of PDL cells. It has been reported that the growth of cells mainly happen at an osmolality of 230 to 400 mosmol/kg and a pH of 6.6 to 7.8 (8,23).…”

“…The longer the exposure of avulsed tooth to dry storage, the worse the prognosis of explantation is. Therefore, many studies aimed to preserve avulsed tooth in proper medium (7)(8)(9)(21)(22)(23). The storage medium should be capable of preserving cell vitality and adherence capacity and also be readily available at the moment of avulsion to allow its rapid access (9,22).…”

Milk activates the expression of cytokines via Nrf2/HO‐1 pathway in human periodontal ligament cells

“…The International Association of Dental Traumatology (IADT) has recommended Hank's balanced salt solution (HBSS) as the storage medium of choice for avulsed teeth (2). Also other storage media for avulsed teeth, such as milk, ViaSpan â , saline, egg white, green tea extract, and propolis, have been reported (4)(5)(6)(7)(8). Although HBSS shows the best result among currently known storage media, at the time of accident, milk and coconut water are recommended as the most commonly available media most conveniently to patients (2,9).…”