A new strategy for fabrication of bone scaffolds using electrospun nano-HAp/PHB fibers and protein hydrogels

Sadat‐Shojai, Mehdi; Khorasani, Mohammad Taghi; Ahmad, Jamal

doi:10.1016/j.cej.2015.12.079

Cited by 108 publications

(72 citation statements)

References 35 publications

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“…Sadat-Shojai e colaboradores [8] confeccionaram membranas tridimensionais de nano-HAp / PHB para regeneração óssea in vivo. Szubert e colaboradores [9] modificaram a superfície do PHB com deposição de hidroxiapatita.…”

Section: Introductionunclassified

Membranas de polihidroxibutirato com hidroxiapatita para utilização como biomaterial

et al. 2017

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RESUMOO avanço da tecnologia dos biomateriais permite desenvolver materiais que auxiliam nos processos reconstrutivos de partes do corpo e incrementam tratamentos e melhora a qualidade de vida dos seres humanos. O Polihidroxibutirato (PHB) é um polímero biocompatível, biodegradável, muito estudado para aplicações médicas e suas propriedades ainda podem ser adequadas para outras diversas aplicações com a incorporação da hidroxiapatita (HA). Esta é uma carga inorgânica biocompatível e bioativa já presente nos tecidos ósseos do organismo. Desta forma, este trabalho teve como objetivo obter membranas composta por PHB e HA para utilização como biomaterial em regeneração tecidual óssea e guiada. Para obtenção das membranas, o PHB foi processado em uma extrusora monorosca de bancada AX-Plásticos (16-mm), com Razão LD=26, a uma taxa de compressão de 1,5, específica para produção de filmes planos. Foram confeccionadas membranas de PHB puro, e os compósitos de PHB com 1%, 2% e 3% de HA. Após obtenção das membranas, as mesmas foram caracterizadas por Difração de raios X (DRX), Microscopia Eletrônica de Varredura (MEV) / Espectroscopia por Energia Dispersiva (EDS), Citotoxicidade e Molhabilidade. Nos difratogramas foi possível observar um perfil de material semicristalino e que a adição da HA diminuiu esse perfil. Nas micrografias foi visualizada uma superfície de um material denso e rugoso. Já no EDS foi evidenciado os elementos químicos presentes no PHB e na HA. O ensaio de citotoxicidade confirmou a viabilidade e consequentemente a biocompatibilidade do material obtido. Já o ensaio de Molhabilidade confirmou o caráter hidrofílico das membranas. Fundamentado nesses resultados, pode-se concluir que a metodologia para obtenção das membranas foi eficaz, sendo as mesmas biocompatíveis e que a quantidade da carga vai depender da indicação de uso do biomaterial em questão.Palavras-chave: PHB. Hidroxiapatita. Biomateriais. Biocompatível. ABSTRACTThe advancement of biomaterials technology allows the development of materials that assist in reconstructive procedures of the body parts and increase treatment and improves the humans quality of life. The polyhydroxybutyrate (PHB) is a biocompatible polymer, biodegradable, widely studied for medical applications and its properties can also be suitable for other various applications with hydroxyapatite (HA) incorporation. This is a bioactive and biocompatible inorganic filler already present in bone tissues. Thus, this study aimed to obtain membranes composed of PHB and HA for use as biomaterials in bone tissue regeneration and guided. To obtain the membranes, PHB was processed in a single screw extruder AXPlastics bench (16 mm), with LD Ratio = 26, a compression ratio of 1.5, specific for the production of flat film. Pure PHB membranes were prepared, and composite PHB with 1%, 2% and 3% HA. After obtaining the membranes, they were characterized by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM) / Energy Dispersive Spectroscopy (EDS) and Cytotoxicity. In the diffraction profi...

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Section: Introductionunclassified

Membranas de polihidroxibutirato com hidroxiapatita para utilização como biomaterial

et al. 2017

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“…A novel study by Zhijiang et al (44) demonstrated that the PHB/cellulose acetate blend nanofiber scaffolds have better biocompatibility and higher proliferation rate of murine osteoblasts than a pure PHB film. In their recent studies, Sadat-Shojai et al describe a new strategy for fabrication of bone scaffolds using electrospun nano-HA/ PHB fibers (33) and electrospun nano-HA/PHB and protein gels (49). According to their results, mechanical properties of the construct were good and murine osteoblasts inside the scaffolds were viable.…”

Section: In Vitro Biocompatibility Of Phb On Bone Cellsmentioning

confidence: 99%

Poly(3-hydroxybutyrate): Promising biomaterial for bone tissue engineering

Dariš

Knez

2019

Acta Pharmaceutica

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Poly(3-hydroxybutyrate) is a natural polymer, produced by different bacteria, with good biocompatibility and biode gradability. Cardiovascular patches, scaffolds in tissue engineering and drug carriers are some of the possible biomedical applications of poly(3-hydroxybutyrate). In the past decade, many researchers examined the different physicochemical modifications of poly(3-hydroxybutyrate) in order to improve its properties for use in the field of bone tissue engineering. Poly(3-hydroxybutyrate) composites with hydroxy apatite and bioglass are intensively tested with animal and human osteoblasts in vitro to provide information about their bio com patibility, biodegradability and osteoinductivity. Good bone regeneration was proven when poly(3-hydroxybutyrate) patches were implanted in vivo in bone tissue of cats, mini pigs and rats. This review summarizes the recent reports of in vitro and in vivo studies of pure poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate) composites with the emphasis on their bioactivity and biocompatibility with bone cells.

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“…As the Gel/HAp component could act as cell carriers and provide ECM‐like microenvironment, therefore the mouse preosteoblasts embedded inside the hydrogel layers significantly proliferated and migrated on 14 d after immersion in simulated body fluid (Figure d). Meanwhile, the degradation of PHB resulted the gradual increase of pore sizes in the electrospun mat layer, thereby promoting the cells migration through the middle layer . Moreover, Ifuku and co‐workers fabricated a new kind calcium phosphate/chitin nanofiber composite hydrogel for the application of bone regeneration.…”

Section: Applications Of Nfhgsmentioning

confidence: 99%

“…d) Fluorescence microscopic images illustrating the infiltration of MC3T3‐E1 preosteoblasts into the nanofiber/hydrogel center layer with the hybrid scaffolds. Reproduced with permission . Copyright 2016, Elsevier.…”

Section: Applications Of Nfhgsmentioning

confidence: 99%

Nanofiber‐Based Hydrogels: Controllable Synthesis and Multifunctional Applications

Duan

Yan

et al. 2018

Macromol. Rapid Commun.

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Nanofiber-based hydrogels (NFHGs) prepared by the combination of traditional hydrogels and novel nanofibers have demonstrated great potential in various application fields, owing to their integrated advantages of superhydrophilicity, high water-holding capacity, good biocompatibility, enhanced mechanical strength, and excellent structural tenability. In this review, a comprehensive overview of the structure design and synthetic strategy of NFHGs derived from electrospinning technique, weaving, freeze-drying, 3D printing, and molecular self-assembling method is provided. The widely researched multifunctional applications, primarily involving tissue engineering, drug delivery, sensing, intelligent actuator, and oil/water separation are also presented. Furthermore, some unsolved scientific issues and possible directions for future development of this field are also intensively discussed.

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A new strategy for fabrication of bone scaffolds using electrospun nano-HAp/PHB fibers and protein hydrogels

Cited by 108 publications

References 35 publications

Membranas de polihidroxibutirato com hidroxiapatita para utilização como biomaterial

Membranas de polihidroxibutirato com hidroxiapatita para utilização como biomaterial

Poly(3-hydroxybutyrate): Promising biomaterial for bone tissue engineering

Nanofiber‐Based Hydrogels: Controllable Synthesis and Multifunctional Applications

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