A new strategy for the analysis of tetracycline residues in foodstuffs by a surface plasmon resonance biosensor

Moeller, Nadine; Mueller-Seitz, Erika; Scholz, Oliver; Hillen, Wolfgang; Bergwerff, Aldert A.; Petz, Michael

doi:10.1007/s00217-006-0392-z

Cited by 29 publications

(15 citation statements)

References 17 publications

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“…Hence, these bacterial strains responded to low levels of tetracyclines by producing β -galactosidase and light or green fluorescent protein [ 105 ]. A similar approach was introduced to a novel methodology in 2007 by Moeller et al [ 106 ] who constructed a biosensor using the tetracycline resistance gene tet O and TetR protein as a regulator. Bahl et al [ 107 ] created an extended range whole cell tetracycline biosensor by inserting tetM, encoding a ribosomal protection protein, into a plasmid that contains a transcriptional fusion between a tetracycline-regulated promoter and the gfp gene.…”

Section: Analytical Methods For Tetracyclines Determination and Rementioning

confidence: 99%

Tetracyclines in Food and Feedingstuffs: From Regulation to Analytical Methods, Bacterial Resistance, and Environmental and Health Implications

Granados-Chinchilla

Rodríguez

2017

Journal of Analytical Methods in Chemistry

252

128

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Antibiotics are widely used as growth promoters in animal husbandry; among them, the tetracyclines are a chemical group of relevance, due to their wide use in agriculture, surpassing in quantities applied almost every other antibiotic family. Seeing the considerable amounts of tetracyclines used worldwide, monitoring of these antibiotics is paramount. Advances must be made in the analysis of antibiotics to assess correct usage and dosage of tetracyclines in food and feedstuffs and possible residues in pertinent environmental samples. The tetracyclines are still considered a clinically relevant group of antibiotics, though dissemination of tolerance and resistance determinants have limited their use. This review focuses on four different aspects: (i) tetracyclines, usage, dosages, and regulatory issues that govern their food-related application, with particular attention to the prohibitions and restrictions that several countries have enforced in recent years by agencies from both the United States and the European Union, (ii) analytical methods for tetracyclines, determination, and residues thereof in feedstuffs and related matrices with an emphasis on the most relevant and novel techniques, including both screening and confirmatory methods, (iii) tetracycline resistance and tetracycline-resistant bacteria in feedstuff, and (iv) environmental and health risks accompanying the use of tetracyclines in animal nutrition. In the last two cases, we discuss the more relevant undesirable effects that tetracyclines exert over bacterial communities and nontarget species including unwanted effects in farmers.

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Section: Analytical Methods For Tetracyclines Determination and Rementioning

confidence: 99%

Tetracyclines in Food and Feedingstuffs: From Regulation to Analytical Methods, Bacterial Resistance, and Environmental and Health Implications

Granados-Chinchilla

Rodríguez

2017

Journal of Analytical Methods in Chemistry

252

128

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“…Adrian et al [145] also presented multidrug family detection in milk, following sample dilution, using a flow-based wavelength-interrogated optical biosensor Clenbuterol Labelled residue competition for antibody (optical-fluorescent) [161] Honey Chloramphenicol Antibody competition with nanoparticle enhancement (optical-SPR) [162] Sulfathiazole Antibody competition (optical-fluorescent) [141] Honey/milk Tetracyclines Receptor competition (optical-SPR) [163] Milk Anti-inflammatory drugs Enzyme inhibition (electrochemical-amperometric) [164] Benzimidazole Antibody competition (optical-SPR) […”

Section: Future For Biosensors For Drug Residuesmentioning

confidence: 99%

Biosensors for the analysis of microbiological and chemical contaminants in food

2012

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Increases in food production and the ever-present threat of food contamination from microbiological and chemical sources have led the food industry and regulators to pursue rapid, inexpensive methods of analysis to safeguard the health and safety of the consumer. Although sophisticated techniques such as chromatography and spectrometry provide more accurate and conclusive results, screening tests allow a much higher throughput of samples at a lower cost and with less operator training, so larger numbers of samples can be analysed. Biosensors combine a biological recognition element (enzyme, antibody, receptor) with a transducer to produce a measurable signal proportional to the extent of interaction between the recognition element and the analyte. The different uses of the biosensing instrumentation available today are extremely varied, with food analysis as an emerging and growing application. The advantages offered by biosensors over other screening methods such as radioimmunoassay, enzyme-linked immunosorbent assay, fluorescence immunoassay and luminescence immunoassay, with respect to food analysis, include automation, improved reproducibility, speed of analysis and real-time analysis. This article will provide a brief footing in history before reviewing the latest developments in biosensor applications for analysis of food contaminants (January 2007 to December 2010), focusing on the detection of pathogens, toxins, pesticides and veterinary drug residues by biosensors, with emphasis on articles showing data in food matrices. The main areas of development common to these groups of contaminants include multiplexing, the ability to simultaneously analyse a sample for more than one contaminant and portability. Biosensors currently have an important role in food safety; further advances in the technology, reagents and sample handling will surely reinforce this position.

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“…Rapid tests (e.g., ELISA and surface plasmon resonance biosensor) are screening methods based on the recognition between antibiotics or receptor proteins specific to a particular type of antibiotics. 11,15−20 They allow fast and sensitive but usually semi-quantitative determination for the antibiotic level. Analytical methods, including high-performance liquid chromatography and mass spectrometry (MS), provide a confirmatory assessment of the antibiotic’s authenticity and a precise qualitative measurement of the antibiotic level.…”

Section: Introductionmentioning

confidence: 99%

Thermostable β-Lactamase Mutant with Its Active Site Conjugated with Fluorescein for Efficient β-Lactam Antibiotic Detection

Au¹,

Tsang²,

So³

et al. 2019

ACS Omega

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Monitoring the β-lactam antibiotic level has been an important task in food industry and clinical practice. Here, we report the development of a fluorescent PenP β-lactamase, PenP-E166Cf/N170Q, for efficient β-lactam antibiotic detection. It was constructed by covalently attaching fluorescein onto the active-site entrance of a thermostable E166Cf/N170Q mutant of a Bacillus licheniformis PenP β-lactamase. It gave a fluorescence turn-on signal toward various β-lactam antibiotics, where the fluorescence enhancement was attributed to the acyl–enzyme complex formed between PenP-E166Cf/N170Q and the β-lactam antibiotic. It demonstrated enhanced signal stability over its parental PenP-E166Cf because of the suppressed hydrolytic activity by the N170Q mutation. Compared with our previously constructed PenPC-E166Cf biosensor, PenP-E166Cf/N170Q was more thermostable and advanced in detecting β-lactams in terms of response time, signal stability, and detection limit. Positive fluorescence signals generated by E166Cf/N170Q in response to the penicillin-containing milk and mouse serum illustrated the feasibility of the biosensor for antibiotic detection in real samples. Taken together, our findings suggest the potential application of PenP-E166Cf/N170Q in biosensing β-lactam antibiotics.

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A new strategy for the analysis of tetracycline residues in foodstuffs by a surface plasmon resonance biosensor

Cited by 29 publications

References 17 publications

Tetracyclines in Food and Feedingstuffs: From Regulation to Analytical Methods, Bacterial Resistance, and Environmental and Health Implications

Tetracyclines in Food and Feedingstuffs: From Regulation to Analytical Methods, Bacterial Resistance, and Environmental and Health Implications

Biosensors for the analysis of microbiological and chemical contaminants in food

Thermostable β-Lactamase Mutant with Its Active Site Conjugated with Fluorescein for Efficient β-Lactam Antibiotic Detection

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