A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye.

Ishiyama, Munetaka; Shiga, Masanobu; Sasamoto, Kazumi; Mizoguchi, Makoto; He, Pingang

doi:10.1248/cpb.41.1118

Cited by 463 publications

(243 citation statements)

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“…Cell viability was assessed with 2-(4-lodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt (WST-1, Wako Pure Chemical, Osaka, Japan), which is based on cleavage of the WST-1 reagent by mitochondrial dehydrogenase of viable cells to formazan dye [17]. Human colon cancer cells preincubated as described above were seeded at a density of 2x10 3 cells/well in 96-well microplates and cultured in 100 µL medium/well for 24 h. Each CLA was dissolved in 10 µl of 5% ethanol solution and then added to the culture.…”

Section: Cell Viabilitymentioning

confidence: 99%

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Beppu

Hosokawa

Tanaka

et al. 2006

The Journal of Nutritional Biochemistry

122

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This study compared the growth inhibitory effects of pure conjugated linoleic acid (CLA) isomers (cis(c)9,c11-CLA, c9,trans(t)11-CLA, t9,t11-CLA, and t10,c12-CLA) on human colon cancer cell lines (Caco-2, HT-29, and DLD-1). When Caco-2 cells were incubated up to 72 h with 200 M each isomer even in the presence of 10% fetal bovine serum (FBS), cell proliferation was inhibited by all CLA isomers in a time dependent manner. The strongest inhibitory effect was shown by t9,t11-CLA, followed by t10,c12-CLA, c9,c11-CLA, and c9,t11-CLA, respectively. The strongest effect of t9,t11-CLA was also observed in other colon cancer cell lines (HT-29 and DLD-1). The order of the inhibitory effect of CLA isomer was confirmed in the presence of 1% FBS. CLA isomers supplemented in the culture medium were readily incorporated into the cellular lipids of Caco-2 and changed their fatty acid composition. The CLA contents in cellular lipids were 26.2+2.7% for t9,t11-CLA, 35.9+0.3% for c9,t11-CLA, and 46.3+0.8% for t10,c12-CLA, respectively. DNA fragmentation was clearly recognized in Caco-2 cells treated with t9,t11-CLA. This apoptotic effect of t9,t11-CLA was dose-and time-dependent. DNA fragmentation was also induced by 9c,11t-CLA and t10,c12-CLA. However, fragmentation levels with both isomers were much lower than that with t9,t11-CLA.t9t11-CLA treatment of Caco-2 cells decreased Bcl-2 levels in association with apoptosis, whereas Bax levels remained unchanged. These results suggest that decreased expression of Bcl-2 by t9t11-CLA might increase the sensitivity of cells to lipid peroxidation and to programmed cell death, apoptosis. AbstractThis study compared the growth inhibitory effects of pure conjugated linoleic acid (CLA) isomers (cis(c)9,c11-CLA, c9,trans(t)11-CLA, t9,t11-CLA, and t10,c12-CLA) on human colon cancer cell lines (Caco-2, HT-29, and DLD-1). When Caco-2 cells were incubated up to 72 h with 200 µM each isomer even in the presence of 10% fetal bovine serum (FBS), cell proliferation was inhibited by all CLA isomers in a time dependent manner. The strongest inhibitory effect was shown by t9,t11-CLA, followed by t10,c12-CLA, c9,c11-CLA, and c9,t11-CLA, respectively. The strongest effect of t9,t11-CLA was also observed in other colon cancer cell lines (HT-29 and DLD-1). The order of the inhibitory effect of CLA isomer was confirmed in the presence of 1% FBS. * Manuscript 2CLA isomers supplemented in the culture medium were readily incorporated into the cellular lipids of Caco-2 and changed their fatty acid composition. The CLA contents in cellular lipids were 26.2+2.7% for t9,t11-CLA, 35.9+0.3% for c9,t11-CLA, and 46.3+0.8% for t10,c12-CLA, respectively. DNA fragmentation was clearly recognized in Caco-2 cells treated with t9,t11-CLA. This apoptotic effect of t9,t11-CLA was doseand time-dependent. DNA fragmentation was also induced by 9c,11t-CLA and t10,c12-CLA. However, fragmentation levels with both isomers were much lower than that with t9,t11-CLA. t9t11-CLA treatment of Caco-2 cells decreased Bcl-2 levels in...

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Section: Cell Viabilitymentioning

confidence: 99%

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Beppu

Hosokawa

Tanaka

et al. 2006

The Journal of Nutritional Biochemistry

122

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“…Wst-1 measures mitochondria1 dehydrogenase activity [21] and refers to 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt (Dojindo. Gaithersburg, MD).…”

Section: Wst-1 CDL Citihilitj Usfuj'mentioning

confidence: 99%

Self‐hardening calcium phosphate composite scaffold for bone tissue engineering

Simon

2004

Journal Orthopaedic Research

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Calcium phosphate cement (CPC) sets in situ to form solid hydroxyapatite, can conform to complex cavity shapes without machining, has excellent osteoconductivity, and is able to be resorbed and replaced by new bone. Therefore, CPC is promising for craniofacial and orthopaedic repairs. However, its low strength and lack of macroporosity limit its use. This study investigated CPC reinforcement with absorbable fibers, the effects of fiber volume fraction on mechanical properties and macroporosity, and the cytotoxicity of CPC–fiber composite. The rationale was that large‐diameter absorbable fibers would initially strengthen the CPC graft, then dissolve to form long cylindrical macropores for colonization by osteoblasts. Flexural strength, work‐of‐fracture (toughness), and elastic modulus were measured vs. fiber volume fraction from 0% (CPC Control without fibers) to 60%. Cell culture was performed with osteoblast‐like cells, and cell viability was quantified using an enzymatic assay. Flexural strength (mean ± SD; n == 6) of CPC with 60% fibers was 13.5 ± 4.4 MPa, three times higher than 3.9 ± 0.5 MPa of CPC Control. Work‐of‐fracture was increased by 182 times. Long cylindrical macropores 293 ± 46 μm in diameter were created in CPC after fiber dissolution, and the CPC–fiber scaffold reached a macroporosity of 55% and a total porosity of 81%. The new CPC–fiber formulation supported cell adhesion, proliferation and viability. The method of using large‐diameter absorbable fibers in bone graft for mechanical properties and formation of long cylindrical macropores for bone ingrowth may be applicable to other tissue engineering materials. Published by Elsevier Ltd. on behalf of Orthopuedic Research Society. © 2003 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.

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“…The effects of rhTNF-␣ and F4614 on the growth of MH134 were compared using a WST-1 assay. 24 Doubling dilutions of rhTNF-␣ or F4614 were prepared in a 96-well flat-bottom plate (Falcon 3072, Becton Dickinson Labware, Lincoln Park, NJ). Tumor cells (2 ϫ 10 3 /well) were then added to obtain a final volume of 100 µL per well.…”

Section: Methodsmentioning

confidence: 99%

A novel human tumor necrosis factor alfa mutein, F4614, inhibits in vitro and in vivo growth of murine and human hepatoma: Implication for immunotherapy of human hepatocellular carcinoma

et al. 1998

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A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye.

Cited by 463 publications

References 0 publications

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Self‐hardening calcium phosphate composite scaffold for bone tissue engineering

A novel human tumor necrosis factor alfa mutein, F4614, inhibits in vitro and in vivo growth of murine and human hepatoma: Implication for immunotherapy of human hepatocellular carcinoma

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