A new surgical technique to treat corneal perforations using amniotic membrane and surgical adhesive

Abstract

The objectives of this study were to compare surgical techniques and the effects of using n-butyl 2-cyanoacrylate and bovine amniotic membrane to repair perforated lesions in corneas. Penetrating keratoplasty was performed in sixty New Zealand White rabbits under general anesthesia. Group 1 (G1) was treated with n-butyl 2-cyanoacrylate, group 2 (G2) received a fragment of amniotic membrane through the anterior chamber and application of n-butyl 2-cyanoacrylate over the lesion, group 3 (G3) was treated with the same technique as G2 with the addition of an amniotic membrane bandage covering the cornea and sutured in the limbus region, and group 4 (G4) was treated with an amniotic membrane sutured to the lesion and an amniotic membrane bandage sutured in the limbus region. Clinical, histological and histomorphometric examinations of the corneas were performed. The membrane acted as a barrier for aqueous humor in G2 and G3, thereby keeping the surface dry for adhesive application; it also prevented the adhesive from contacting intraocular structures. The groups treated with amniotic membrane and surgical adhesive showed better results than the groups treated with either material alone. Thus, the combination of the membrane with the adhesive is recommended for this type of lesion.

Bacterial cellulose and bacterial cellulose/polycaprolactone composite as tissue substitutes in rabbits' cornea

“…3C and 3F). This differs from the results of Pontes et al (2014), who described equivalence between the two types of collagen and organization of fibers at 30 days in all groups evaluated. Thus, it is suggested that the presence of BC/PCL and BC membranes slowed corneal stroma healing process.…”

“…At 21 days, there was a higher proportion of immature collagen fibers and disorganization of collagen fibers in all groups (Figs. 3B and 3E), agreeing with the results of Pontes et al (2014) that reported prevalence of immature collagen at 21 days after application of amniotic membrane and n-butyl-2-cyanoacrylate to treat penetrating injuries in rabbits' corneas. In the cornea, type I collagen is predominant, since type III is found in small quantities (Qazi et al 2010).…”

“…Figure 1 shows the surgical procedure used in this study. Briefly a superficial keratectomy was made in the center of the cornea, thus reaching visual axis, in a manner similar to that described by Pontes et al (2014). However, in this work, the ulcer was made with 5.0 mm in diameter and 0.2 mm thick using a disposable biopsy punch (Kolpast, Brazil) and an interlayer pocket, from the base of the ulcer was made with a delamination spatula that advanced about 3.0mm toward the limbus.…”

Microbiological viability of bovine amniotic membrane stored in glycerin 99% at room temperature for 48 months

“…Because our group frequently uses the amniotic membrane in research and in the hospital routine (Pontes et al, 2011;Pontes et al, 2014), it was important to evaluate this medium aiming at creating a bank of this tissue to be used in the long term.…”

“…The amnion was manually separated from the chorion ( Figure 1A) (Kim & Tseng, 1995), mounted, epithelial face upwards, onto nitrocellulose paper ( Figure 1B), and cut into the size of 1.0 cm 2 . Subsequently, the fragment was placed in a sterile glass vial and covered with sterile glycerin 99% ( Figure 1C) (Pontes et al, 2014).…”