A New Synthetic Diet Increased Bone Mineral Density and Strength of Ovariectomized Rat

Abstract

Bone mineral density; Bone strength; Micro-CT Measurements of bone mineral density (BMD) and threedimensional color mapping (3D-map)Micro-CT settings: Femoral micro-CT images (Figure 1A) were acquired setting the measurement range at the middle point region of the full femoral length measured using calipers (Figure 1B). The micro-CT was carried out using a R_mCT (Rigaku Co., Japan). The value of BMD was measured using TRI/3D-Bon BMD software (TRI/3D; Ratoc System Engineering Co., Japan). Three-dimensional color mapping (3D-map) of the BMD distributions was carried out using TRI/3D image analysis software. Measurements of bone strengthBone strength was measured using an Instron type testing machine system (Figure 1C), TG-5kN, Minebea Co., Japan). Bending force was loaded on the middle point of the bone length at 5.0 mm/min. Statistical analysisThe means and standard deviations of body weight, BMD, and bone strength were calculated for each group and compared using Student's t-test.

Historical Review of Biological Apatite Crystallography

“…A new synthetic bone mineral (SBM) diet was d e ve l o p e d a n d th e e ffe c t was c o n fi r me d b y a n ima l experiment [148][149][150] . …”