A new system for paper chromatography of polar steroids

17-Hydroxycorticoids in Mouse Adrenal Incubates

“…Had this occurred because of an inhibition of 11/,-hydroxylation by andro¬ gens, then an increase in 11-deoxycortisol might have been anticipated. This compound was never identified in our studies although this steroid has been shown to separate well from corticosterone in the isopropyl ether-ethylene glycol system (Touchstone et al 1962). Investigation of mechanisms involved in the inhibition of 17-hydroxycorticoid secretion by androgens is in progress.…”

“…The systems used were toluene-propylene glycol for 96 hours and isopropyl ether-ethylene glycol for 42 hours (Burton et at. 1951;Touchstone et al 1962). Good separation of cortisol was achieved.…”

Chromatography

“…Modified solvent systems have been described for sterols (443,906), vitamins D (439, 919), steroidal saponins (1187) and alkaloids (1414,1516), bile acids (1180), cardiac glycosides (593,1606,1717), and steroids related to the estrogenic (314,569,768,955,1360,1596), androgenic (44,239,452,782,955,1304,1376), progestational (955,1177), and adrenocortical (220,955,1454,1596) hormones, particularly aldosterone (733,1189).…”

Conjugated and Free Estriol in Corpus Luteum of Human Pregnancy*

“…Estriol fractions from the alumina column were subjected to paper chromatography on Whatman No. 1 paper strips using the isopropyl ether-ethylene glycol system with development for 36 hours (Touchstone et al, 1962). Zones were located by treatment with the ferric chloride-potassium ferricyanide reagent.…”