A new technique for highly sensitive detection of superoxide dismutase activity by chemiluminescence

Corbisier, Philippe; Houbion, Andrée; Remacle, José

doi:10.1016/0003-2697(87)90392-7

Cited by 84 publications

(52 citation statements)

References 23 publications

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“…Treatment of the cell suspension with 1 mM DDC was necessary to measure the accumulation of O,-, because 0,-was otherwise very rapidly converted to H,O, by SOD. DDC at 0.63 mM gives nearly complete inhibition of the activity of purified SOD or of SOD in cellular supernatant, and it does not interfere with the light signal of LCDC (Corbisier et al, 1987). Phytophthora elicitor stimulated the generation of O,-by rose cells (Figs.…”

Section: Dlscusslonmentioning

confidence: 92%

“…The accumulated amount of O,-in the cell suspensions was measured by the chemiluminescence of lucigenin, which is specific for O,- (Corbisier et al, 1987). The assay was conducted in a total volume of 2 mL by placing 0.2 mL of cell suspension and 0.2 mL of 1 mM lucigenin in 0.1 M Gly-NaOH buffer (pH 9.0) containing 1 mM EDTA and 1 mM sodium salicylate.…”

Section: Chemiluminescence Assay For O-mentioning

confidence: 99%

“…The inhibition of LMDC by DDC also implies that rose cells increased their rate of synthesis of O,-in response to elicitor treatment. More direct evidence for the synthesis of O,-was obtained by assaying O,-using LCDC, which is specific for O,- (Corbisier et al, 1987). Treatment of the cell suspension with 1 mM DDC was necessary to measure the accumulation of O,-, because 0,-was otherwise very rapidly converted to H,O, by SOD.…”

Section: Dlscusslonmentioning

confidence: 99%

See 2 more Smart Citations

Plasma Membrane Redox Enzyme Is Involved in the Synthesis of O2- and H2O2 by Phytophthora Elicitor-Stimulated Rose Cells

1995

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Section: Dlscusslonmentioning

confidence: 92%

Section: Chemiluminescence Assay For O-mentioning

confidence: 99%

Section: Dlscusslonmentioning

confidence: 99%

See 1 more Smart Citation

Plasma Membrane Redox Enzyme Is Involved in the Synthesis of O2- and H2O2 by Phytophthora Elicitor-Stimulated Rose Cells

1995

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“…The chemiluminescence was detected in the luminometer, which detects real-time luminosity, or by using the single-channel mode in a scintillation spectrometer. This procedure has been considered a specific assay of superoxide (Corbisier et al, 1987), but recent reports (Liochev and Fridovich, 1997;Vásques-Vivar et al, 1997) indicate that reduced lucigenin can react with O 2 to produce superoxide. Thus, enzymatic or nonenzymatic reactions that reduce lucigenin can give a false-positive reading.…”

Section: Lucigenin Assay For Superoxidementioning

confidence: 99%

Comparative Biochemistry of the Oxidative Burst Produced by Rose and French Bean Cells Reveals Two Distinct Mechanisms1

et al. 1998

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“…Plasma xanthine oxidase activity Plasma xanthine oxidase activity was determined with chemiluminescence as described by Corbisier, Houbion & Remacle (1987).…”

Section: Muscle Lipid Peroxidationmentioning

confidence: 99%

Xanthine oxidase in human skeletal muscle following eccentric exercise: a role in inflammation.

Hellsten¹,

Frandsen²,

Orthenblad³

et al. 1997

The Journal of Physiology

214

147

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1. The present study tested the hypothesis that the level of xanthine oxidase is elevated in injured human skeletal muscle in association with inflammatory events. Seven male subjects performed five bouts of strenuous one-legged eccentric exercise. Muscle biopsies from both the exercised and the control leg, together with venous blood samples, were obtained prior to exercise and at 45 min, 24, 48 and 96 h after exercise. The time courses of xanthine oxidase immunoreactivity and indicators of muscle damage and inflammation were examined. 2. The number of xanthine oxidase structures observed by immunohistological methods in the exercised muscle was up to eightfold higher than control from day 1 to day 4 after exercise (P < 0 05). The increase was attributed to an enhanced expression of xanthine oxidase in microvascular endothelial cells and an invasion of leucocytes containing xanthine oxidase. 3. The concentration of plasma interleukin-6 was significantly higher 90 min after exercise than before exercise (P < 0 05) and remained higher than pre-exercise levels throughout the 4 days. On day 4 the plasma creatine kinase activity was approximately 150-fold higher (P < 0 05) than resting levels. 4. Despite the increase in xanthine oxidase in the muscle there were no detectable changes in the levels of muscle malondialdehyde or in plasma antioxidant capacity up to 4 days postexercise. 5. It is concluded that eccentric exercise leads to an increased level of xanthine oxidase in human muscle and that the increase is associated with secondary inflammatory processes. The increase in xanthine oxidase in the muscle occurs mainly in microvascular endothelial cells, but occurs also via infiltrating leucocytes containing xanthine oxidase. A role for leucocytes in xanthine oxidase induction in endothelium is proposed.It is well known that inflammatory events involve the generation of free radicals via NADPH oxidase and myeloperoxidase in immunoparticipating cells for the purpose of bacterial defence and phagocytosis. More recent evidence suggests that superoxide radicals (O2-) are also of importance in neutrophil attraction and neutrophil adherence to endothelium

show abstract

A new technique for highly sensitive detection of superoxide dismutase activity by chemiluminescence

Cited by 84 publications

References 23 publications

Plasma Membrane Redox Enzyme Is Involved in the Synthesis of O2- and H2O2 by Phytophthora Elicitor-Stimulated Rose Cells

Plasma Membrane Redox Enzyme Is Involved in the Synthesis of O2- and H2O2 by Phytophthora Elicitor-Stimulated Rose Cells

Comparative Biochemistry of the Oxidative Burst Produced by Rose and French Bean Cells Reveals Two Distinct Mechanisms1

Xanthine oxidase in human skeletal muscle following eccentric exercise: a role in inflammation.

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