A new technique for monoconidial culture of the most aggressive isolate in a given population of Bipolaris sorokiniana, cause of foliar spot blotch in wheat and barley

Kumar, Dharmendra; Chand, Ramesh; Prasad, L. C.; Joshi, Arun Kumar

doi:10.1007/s11274-007-9410-y

Cited by 29 publications

(16 citation statements)

References 13 publications

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“…SD40 is one of the most virulent isolate predominantly found in United States Great Plains areas and routinely used to screen breeding materials (provided by Dr. Shaukat Ali, SDSU). The isolate was originally derived from a single spore and method of its isolation and cultivation is well described by Kumar et al (2007) . This fungus is easily identifiable based on the color morphology and number of nuclei in lab conditions ( Shoemaker, 1959 ).…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Association Study for Spot Blotch Resistance in Hard Winter Wheat

et al. 2018

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Spot blotch (SB) caused by Cochliobolus sativus (anamorph: Bipolaris sorokiniana) is an economically important disease of wheat worldwide. Under a severe epidemic condition, the disease can cause yield losses up to 70%. Previous approaches like bi-parental mapping for identifying SB resistant genes/QTLs exploited only a limited portion of the available genetic diversity with a lower capacity to detect polygenic traits, and had a lower marker density. In this study, we performed genome-wide association study (GWAS) for SB resistance in hard winter wheat association mapping panel (HWWAMP) of 294 genotypes. The HWWAMP was evaluated for response to B. sorokiniana (isolate SD40), and a range of reactions was observed with 10 resistant, 38 moderately resistant, 120 moderately resistant- moderately susceptible, 111 moderately susceptible, and 15 susceptible genotypes. GWAS using 15,590 high-quality SNPs and 294 genotypes we identified six QTLs (p = <0.001) on chromosomes 2D, 3A, 4A, 4B, 5A, and 7B that collectively explained 30% of the total variation for SB resistance. Highly associated SNPs were identified for all six QTLs, QSb.sdsu-2D.1 (SNP: Kukri_c31121_1460, R2 = 4%), QSb.sdsu-3A.1 (SNP: Excalibur_c46082_440, R2 = 4%), QSb.sdsu-4A.1 (SNP: IWA8475, R2 = 5.5%), QSb.sdsu-4B.1 (SNP: Excalibur_rep_c79414_306, R2 = 4%), QSb.sdsu-5A.1 (SNP: Kukri_rep_c104877_2166, R2 = 6%), and QSb.sdsu-7B.1 (SNP: TA005844-0160, R2 = 6%). Our study not only validates three (2D, 5A, and 7B) genomic regions identified in previous studies but also provides highly associated SNP markers for marker assisted selection. In addition, we identified three novel QTLs (QSb.sdsu-3A.1, QSb.sdsu-4A.1, and QSb.sdsu-4B.1) for SB resistance in wheat. Gene annotation analysis of the candidate regions identified nine NBS-LRR and 38 other plant defense-related protein families across multiple QTLs, and these could be used for fine mapping and further characterization of SB resistance in wheat. Comparative analysis with barley indicated the SB resistance locus on wheat chromosomes 2D, 3A, 5A, and 7B identified in our study are syntenic to the previously identified SB resistance locus on chromosomes 2H, 3H, 5H, and 7H in barley. The 10 highly resistant genotypes and SNP markers identified in our study could be very useful resources for breeding of SB resistance in wheat.

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Section: Methodsmentioning

confidence: 99%

Genome-Wide Association Study for Spot Blotch Resistance in Hard Winter Wheat

et al. 2018

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“…Shoem.]. It is one of the major concerns in South Asia including China, Nepal, Pakistan, Bangladesh and the humid north eastern regions of India (Kumar et al, 2007;Chand et al, 2008;Singh et al, 2009;Vaish et al, 2011;Prasad et al, 2013). In addition, SB is also considered as a serious threat to barley production in the upper Midwest of the United States and the prairie provinces of Canada (Clark, 1979;Ghazvini and Tekauz, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genome Wide Association Mapping of Spot Blotch Resistance at Seedling and Adult Plant Stages in Barley

Visioni¹,

Rehman²,

Viash

et al. 2020

Front. Plant Sci.

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Barley spot blotch (SB) caused by Cochliobolus sativus is one of the major constrains to barley production in warmer regions worldwide. The study was undertaken to identify and estimate effects of loci underlying quantitative resistance to SB at the seedling and adult plant stages. A panel of 261 high input (HI-AM) barley genotypes consisting of released cultivars, advanced breeding lines, and landraces, was screened for resistance to SB. The seedling resistance screening was conducted using two virulent isolates from Morocco (ICSB3 and SB54) while the adult plant stage resistance was evaluated at two hot spot locations, Faizabad and Varanasi, in India under artificial inoculation using a mixture of prevalent virulent isolates. The HI-AM panel was genotyped using DArT-Seq high-throughput genotyping platform. Genome wide association mapping (GWAM) was conducted using 13,182 PAV and 6,311 SNP markers, for seedling and adult plant resistance. Both GLM and MLM model were employed in TASSEL (v 5.0) using principal component analysis and Kinship Matrix as covariates. Final disease rating and Area Under Disease Progress Curve (AUDPC) were used for the evaluation of adult stage plant resistance. The GWAM analysis indicated 23 QTL at the seedling stage (14 for isolate ICSB3 and 9 for isolate SB54), while 15 QTL were detected at the adult plant stage resistance (6 at Faizabad and 9 at Varanasi) and 5 for AUDPC based resistance at Varanasi. Common QTL at seedling and adult plant stages were found across all barley chromosomes. Seedling stage QTL explained together 73.24% of the variance for seedling resistance to isolate ICSB3 and 49.26% for isolate SB54, whereas, QTL for adult plant stage resistance explained together 38.32%, 44.09% and 26.42% of the variance at Faizabad and Varanasi and AUDPC at Varanasi, respectively. Several QTL identified in this study were also reported in previous studies using bi-parental and association mapping populations, corroborating our results. The promising QTL detected at both stages, once validated, can be used for marker assisted selection (MAS) in SB resistance barley breeding program.

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“…Total 10 samples were drawn from each genotype and 10 isolates of each population group were characterized further. Pure cultures were established by single spore isolation technique [16]. Cultures were maintained at 25°C by transfer on PDA and were revived at monthly interval.…”

Section: Methodsmentioning

confidence: 99%

Emergence of Aggressive Population in the Bipolaris sorokiniana of Barley (Hordeum vulgare L.) Through Anastomosis

Bashyal

Chand

Aggarwal

2014

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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Bipolaris sorokiniana is a hemibiotrophic pathogenic fungus responsible for spot blotch of wheat and barley. Evolution process of most virulent population of B. sorokiniana of barley was studied through colony morphology, melanin content, spore production, pathogenicity, nuclear condition and anastomosis. Bridge (H) type and hyphal tip anastomosis were observed between black and white isolates. Maximum number of nuclei (4.14 nuclei cell -1 ) were present in mixed isolates followed by white isolate (2.43 nuclei cell -1 ) and black isolate (1.14 nuclei cell -1 ). Results indicated that aggressive population in B. sorokiniana was evolved by the variations in the nucleus due to anastomosis.

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A new technique for monoconidial culture of the most aggressive isolate in a given population of Bipolaris sorokiniana, cause of foliar spot blotch in wheat and barley

Cited by 29 publications

References 13 publications

Genome-Wide Association Study for Spot Blotch Resistance in Hard Winter Wheat

Genome-Wide Association Study for Spot Blotch Resistance in Hard Winter Wheat

Genome Wide Association Mapping of Spot Blotch Resistance at Seedling and Adult Plant Stages in Barley

Emergence of Aggressive Population in the Bipolaris sorokiniana of Barley (Hordeum vulgare L.) Through Anastomosis

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