A New Tool for Oligonulceotide Import into Cells

Leslie, Daniel C.; Landers, James P.

doi:10.1373/clinchem.2008.113019

Cited by 2 publications

(1 citation statement)

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“…Gold nanoparticles (AuNPs) are important nanomaterials in biomedicine where they can be used to achieve drug delivery and photodynamic therapy [1-6]. For biomedical applications, a thorough understanding of the mechanisms of AuNP cellular entry and exit is required.…”

Section: Introductionmentioning

confidence: 99%

Size-dependent endocytosis of gold nanoparticles studied by three-dimensional mapping of plasmonic scattering images

Wang

Lee

Chiou³

et al. 2010

J Nanobiotechnol

253

161

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BackgroundUnderstanding the endocytosis process of gold nanoparticles (AuNPs) is important for the drug delivery and photodynamic therapy applications. The endocytosis in living cells is usually studied by fluorescent microscopy. The fluorescent labeling suffers from photobleaching. Besides, quantitative estimation of the cellular uptake is not easy. In this paper, the size-dependent endocytosis of AuNPs was investigated by using plasmonic scattering images without any labeling.ResultsThe scattering images of AuNPs and the vesicles were mapped by using an optical sectioning microscopy with dark-field illumination. AuNPs have large optical scatterings at 550-600 nm wavelengths due to localized surface plasmon resonances. Using an enhanced contrast between yellow and blue CCD images, AuNPs can be well distinguished from cellular organelles. The tracking of AuNPs coated with aptamers for surface mucin glycoprotein shows that AuNPs attached to extracellular matrix and moved towards center of the cell. Most 75-nm-AuNPs moved to the top of cells, while many 45-nm-AuNPs entered cells through endocytosis and accumulated in endocytic vesicles. The amounts of cellular uptake decreased with the increase of particle size.ConclusionsWe quantitatively studied the endocytosis of AuNPs with different sizes in various cancer cells. The plasmonic scattering images confirm the size-dependent endocytosis of AuNPs. The 45-nm-AuNP is better for drug delivery due to its higher uptake rate. On the other hand, large AuNPs are immobilized on the cell membrane. They can be used to reconstruct the cell morphology.

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Section: Introductionmentioning

confidence: 99%