A New Transcutaneous Method for Breast Cancer Detection with Dogs

Thuleau, Aurélie; Gilbert, Caroline; Bauër, Pierre; Alran, S.; Fourchotte, Virginie; Guillot, Eugénie; Vincent‐Salomon, Anne; Kerihuel, Jean-Charles; Dugay, J.; Semetey, Vincent; Kriegel, Irène; Fromantin, Isabelle

doi:10.1159/000492895

Cited by 18 publications

(21 citation statements)

References 11 publications

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“…If the sampling protocol is made at home with no supervision, risk of error can occur, leading to poor samples quality. Thuleau et al (2018) report that to sample skin secretion they asked patients and volunteers to shower with an odourless soap, before sleeping with a cotton pad on the breast overnight. In this case, researchers cannot be sure that the person has followed each step correctly or that no incident occurred.…”

Section: Sampling Protocolsmentioning

confidence: 99%

“…They are well described primarily for two types of samples: exhaled air and skin secretions. For instance, Thuleau et al 2018 report that all patients and controls must shower with the identical odourless soap before skin secretions sampling (49). As well, some studies add information about fasting requirements prior to sampling, to limit biases.…”

Section: Sampling Materials and Protocolsmentioning

confidence: 99%

“…An inaccurate reference test might lead to sample status errors and mislead the detector. For instance, Thuleau et al (2018) Given these values, it can be considered that 20-30% of these control patients with negative prostate biopsies had prostate cancer.…”

Section: Patient and Control Selection: Reference Test And Populations Matchingmentioning

confidence: 99%

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Remote Medical Scent Detection of Cancer and Infectious Diseases With Dogs and Rats: a Systematic Review

Bauër

Leemans

Audureau

et al. 2021

Preprint

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Background: Remote medical scent detection of cancer and infectious diseases with dogs and rats has been an increasing field of research these last 20 years. If validated, the possibility of implementing such a technique in the clinic raises many hopes. This systematic review was performed to determine the evidence and performance of such methods and assess their potential relevance in the clinic.Methods: Pubmed and Web of Science databases were independently searched based on PRISMA standards. We included studies aiming at detecting cancers and infectious diseases affecting humans with dogs or rats. We excluded studies using other animals, studies aiming to detect agricultural diseases, diseases affecting animals, and others such as diabetes and neurodegenerative diseases. Only original articles were included. Data about patients’ selection, samples, animal characteristics, animal training and testing configurations, and performances were recorded.Results: A total of 62 studies were included. Sensitivity and specificity varied a lot among studies: While some publications report low sensitivities of 17% and specificities around 29%, others achieve rates of 100% sensitivity and specificity. Only 6 studies were evaluated in a double-blind screening like situation. In general, the risk of performance bias was high in most evaluated studies, and the quality of the evidence found was low.Conclusions: Medical detection using animals’ sense of smell lacks evidence and performances so far to be applied in the clinic. What odours the animals detect is not well understood. Further research should be conducted, focusing on patient selection, samples (choice of materials, standardization), and testing conditions. Interpolations of such results to free running detection (direct contact with humans) should be taken with extreme caution.

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Section: Sampling Protocolsmentioning

confidence: 99%

Section: Sampling Materials and Protocolsmentioning

confidence: 99%

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Remote Medical Scent Detection of Cancer and Infectious Diseases With Dogs and Rats: a Systematic Review

Bauër

Leemans

Audureau

et al. 2021

Preprint

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“…Indeed, to control the quality of raw materials or final food and perfume products, industries often have recourse to human sensory panels. Trained dogs are commonly employed for security controls or even for the detection of diseases such as prostate and breast cancers [6,7]. Although very sensitive and efficient for field studies, the use of the biological nose presents several drawbacks.…”

Section: Introductionmentioning

confidence: 99%

An Overview of Artificial Olfaction Systems with a Focus on Surface Plasmon Resonance for the Analysis of Volatile Organic Compounds

et al. 2021

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The last three decades have witnessed an increasing demand for novel analytical tools for the analysis of gases including odorants and volatile organic compounds (VOCs) in various domains. Traditional techniques such as gas chromatography coupled with mass spectrometry, although very efficient, present several drawbacks. Such a context has incited the research and industrial communities to work on the development of alternative technologies such as artificial olfaction systems, including gas sensors, olfactory biosensors and electronic noses (eNs). A wide variety of these systems have been designed using chemiresistive, electrochemical, acoustic or optical transducers. Among optical transduction systems, surface plasmon resonance (SPR) has been extensively studied thanks to its attractive features (high sensitivity, label free, real-time measurements). In this paper, we present an overview of the advances in the development of artificial olfaction systems with a focus on their development based on propagating SPR with different coupling configurations, including prism coupler, wave guide, and grating.

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“…These have included studies in the detection of lung, breast, ovarian, bladder, and prostate cancer [8][9][10][11][12][13][14][15][16][17]. In 2015, Taverna et al published a pivotal paper on the canine detection of prostate cancer from urine [18].…”

Section: Introductionmentioning

confidence: 99%

Feasibility of Integrating Canine Olfaction with Chemical and Microbial Profiling of Urine to Detect Lethal Prostate Cancer

Guest

Harris

Sfanos

et al. 2020

Preprint

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Prostate cancer is the second leading cause of cancer death in men in the developed world. A more sensitive and specific detection strategy for lethal prostate cancer beyond serum prostate specific antigen (PSA) population screening is urgently needed. Diagnosis by canine olfaction, using dogs trained to detect cancer by smell, has been shown to be both specific and sensitive. While dogs themselves are impractical as scalable diagnostic sensors, machine olfaction for cancer detection is testable. However, studies bridging the divide between clinical diagnostic techniques, artificial intelligence, and molecular analysis remains difficult due to the significant divide between these disciplines. We tested the clinical feasibility of a cross-disciplinary, integrative approach to early prostate cancer biosensing in urine using trained canine olfaction, volatile organic compound (VOC) analysis by gas chromatography-mass spectroscopy (GC-MS) artificial neural network (ANN)-assisted examination, and microbial profiling in a double-blinded pilot study. Two dogs were trained to detect Gleason 9 prostate cancer in urine collected from biopsy-confirmed patients. Biopsy-negative controls were used to assess canine specificity as prostate cancer biodetectors. Urine samples were simultaneously analyzed for their VOC content in headspace via GC-MS and urinary microbiota content via 16S rDNA Illumina sequencing. In addition, the dogs’ diagnoses were used to train an ANN to detect significant peaks in the GC-MS data. The canine olfaction system was 71% sensitive and between 70-76% specific at detecting Gleason 9 prostate cancer. We have also confirmed VOC differences by GC-MS and microbiota differences by 16S rDNA sequencing between cancer positive and biopsy-negative controls. Furthermore, the trained ANN identified regions of interest in the GC-MS data, informed by the canine diagnoses. Methodology and feasibility are established to inform larger-scale studies using canine olfaction, urinary VOCs, and urinary microbiota profiling to develop machine olfaction diagnostic tools. Scalable multi-disciplinary tools may then be compared to PSA screening for earlier, non-invasive, more specific and sensitive detection of clinically aggressive prostate cancers in urine samples.

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A New Transcutaneous Method for Breast Cancer Detection with Dogs

Cited by 18 publications

References 11 publications

Remote Medical Scent Detection of Cancer and Infectious Diseases With Dogs and Rats: a Systematic Review

Remote Medical Scent Detection of Cancer and Infectious Diseases With Dogs and Rats: a Systematic Review

An Overview of Artificial Olfaction Systems with a Focus on Surface Plasmon Resonance for the Analysis of Volatile Organic Compounds

Feasibility of Integrating Canine Olfaction with Chemical and Microbial Profiling of Urine to Detect Lethal Prostate Cancer

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