A New Twist on Transposons

Eckardt, Nancy A.

doi:10.1105/tpc.150210

Cited by 5 publications

References 15 publications

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A novel class of Helitron- related transposable elements in maize contain portions of multiple pseudogenes

et al. 2005

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We recently described a maize mutant caused by an insertion of a Helitron type transposable element (Lal, S.K., Giroux, M.J., Brendel, V., Vallejos, E. and Hannah, L.C., 2003, Plant Cell, 15: 381-391). Here we describe another Helitron insertion in the barren stalk1 gene of maize. The termini of a 6525 bp insertion in the proximal promoter region of the mutant reference allele of maize barren stalk1 gene (ba1-ref) shares striking similarity to the Helitron insertion we reported in the Shrunken-2 gene. This insertion is embedded with pseudogenes that differ from the pseudogenes discovered in the mutant Shrunken-2 insertion. Using the common terminal ends of the mutant insertions as a query, we discovered other Helitron insertions in maize BAC clones. Based on the comparison of the insertion site and PCR amplified genomic sequences, these elements inserted between AT dinucleotides. These putative non-autonomous Helitron insertions completely lacked sequences similar to RPA (replication protein A) and DNA Helicases reported in other species. A blastn analysis indicated that both the 5' and 3' termini of Helitrons are repeated in the maize genome. These data provide strong evidence that Helitron type transposable elements are active and may have played an essential role in the evolution and expansion of the maize genome.

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A novel class of Helitron- related transposable elements in maize contain portions of multiple pseudogenes

et al. 2005

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Molecular mapping and candidate gene analysis of the semi-dominant gene Vestigial glume1 in maize

et al. 2019

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Model-based identification of Helitrons results in a new classification of their families in Arabidopsis thaliana

Tempel

Nicolas

Amrani

et al. 2007

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Helitrons are a class of prolific transposable elements in the Arabidopsis thaliana genome. Although 37 families were identified after the recent discovery of Helitrons, no systematic classification is available because of the high variability of helitronic sequences. Since transposition proteins are assumed to interact with Helitron termini, a Helitron model was formalized based on terminus characterization in order to carry out an exhaustive analysis of all possible combinations of the pairs of termini present. This combinatorics approach resulted in the discovery of a number of new Helitron elements corresponding to termini associations from distinct previously-described Helitron families. The occurrence matrix of termini combinations yielded a structure that revealed clusters of Helitron families.

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A New Twist on Transposons

Cited by 5 publications

References 15 publications

A novel class of Helitron- related transposable elements in maize contain portions of multiple pseudogenes

A novel class of Helitron- related transposable elements in maize contain portions of multiple pseudogenes

Molecular mapping and candidate gene analysis of the semi-dominant gene Vestigial glume1 in maize

Model-based identification of Helitrons results in a new classification of their families in Arabidopsis thaliana

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