A new window into fish welfare: A proteomic discovery study of stress biomarkers in the skin mucus of gilthead seabream (Sparus aurata)

Magalhães, Cláudia Raposo de; Farinha, Ana Paula; Carrilho, Raquel; Schrama, Denise; Cerqueira, Marco; Rodrigues, Pedro M.

doi:10.1016/j.jprot.2023.104904

Cited by 4 publications

(1 citation statement)

References 87 publications

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“…Skin is the organ that is the most directly exposed to various environmental stimuli, such as microplastics and hypoxia [ 59 , 60 ]. As such, its response to stressors may be more heightened compared to other tissues.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Stress Responses of Coreius guichenoti to Microplastics with Different Particle Sizes

Wu,

Qiu,

Lin

et al. 2023

Toxics

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The wild population resources of Coreius guichenoti have sharply declined in recent decades, and any negative factors may have a significant impact on their survival. In this study, the enzymatic stress responses of C. guichenoti to 25 and 48 μm polyethylene fragments were explored for the first time. This was achieved by evaluating the changes in physiological and biochemical indicators of the species in response to the environmental stimuli of microplastics. In this study, we observed an early stress response in the external tissues of C. guichenoti following exposure to microplastics. The TP content in skin and muscle and the MDA content in skin, gill and muscle initially showed a significant increase. The skin, gill, and muscle exhibited greater stress responses to M5 particles, whereas M3 particles caused a greater response in the intestine and especially the liver. After the removal of microplastic exposure, the stress state of the C. guichenoti would be alleviated in a short period, but it could not fully recover to the pre-exposure level. In summary, microplastics pose a significant threat to C. guichenoti. While their negative effects can be alleviated by the removal of microplastics exposure, full recovery does not occur in a short period. Continuous monitoring of microplastics in natural waters and targeted aquatic ecological restoration are essential to ensure the normal growth and reproduction of the wild population of C. guichenoti.

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Section: Discussionmentioning

confidence: 99%

Enzymatic Stress Responses of Coreius guichenoti to Microplastics with Different Particle Sizes

Wu,

Qiu,

Lin

et al. 2023

Toxics

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Discrimination of hypoxia tolerant and intolerant hybrid groupers using surface-enhanced Raman spectroscopy of epidermal mucus

Leong,

Mujar,

Mazlan

et al. 2025

Aquaculture

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Embracing prospects for reducing the numbers of animals used in aquaculture research

Lazado,

Ytteborg,

Noble

2024

Journal of Fish Biology

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The principles of three Rs—REPLACEMENT, REDUCTION, and REFINEMENT—govern the protection and use of animals, including fish, for research purposes in the European Union and Norway. In this paper, we discuss some straightforward steps to simplify the delivery of these principles at the idea stage and adapt some of these examples for conducting fish trials related to health and welfare. Although some of the approaches are well established in other animal science arenas, we believe there can be a timely recap of their key facets. We discuss a number of simple strategies to emphasize how a reduction in fish numbers can be achieved from initial project conception to implementation, highlighting not only their advantages but also their limitations. We also highlight the role that funding agencies can play in the implementation of the 3R principles in aquaculture research. These simple points can be used in frameworks to initiate a broader and dynamic intersectoral dialogue among stakeholders of aquaculture research on how to promote ethics and embrace opportunities for this within the tenets of the 3Rs.

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A new window into fish welfare: A proteomic discovery study of stress biomarkers in the skin mucus of gilthead seabream (Sparus aurata)

Cited by 4 publications

References 87 publications

Enzymatic Stress Responses of Coreius guichenoti to Microplastics with Different Particle Sizes

Enzymatic Stress Responses of Coreius guichenoti to Microplastics with Different Particle Sizes

Discrimination of hypoxia tolerant and intolerant hybrid groupers using surface-enhanced Raman spectroscopy of epidermal mucus

Embracing prospects for reducing the numbers of animals used in aquaculture research

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