2021
DOI: 10.1039/d1ra04321h
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A newly designed sticker-plastic sheet platform and smartphone-based digital imaging for protein assay in food samples with downscaling Kjeldahl digestion

Abstract: The combination of a newly designed sticker-plastic sheet platform, smartphone-based digital imaging and down scaled Kjeldahl digestion is proposed for the determination of protein contents in food samples.

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Cited by 16 publications
(5 citation statements)
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“…A value of R = 0, G = 0, B = 0 refers to pure black while R = 255, G = 255, B = 255 is pure white. With this system, the unique combination of R, G and B values ​​provides these conditions to provide millions of different hues, saturations and hues [ [6] , [7] , [8] ]. This range of colors provides a very favorable database for quantitative measurements.…”
Section: Methods Detailsmentioning
confidence: 99%
“…A value of R = 0, G = 0, B = 0 refers to pure black while R = 255, G = 255, B = 255 is pure white. With this system, the unique combination of R, G and B values ​​provides these conditions to provide millions of different hues, saturations and hues [ [6] , [7] , [8] ]. This range of colors provides a very favorable database for quantitative measurements.…”
Section: Methods Detailsmentioning
confidence: 99%
“…The in-house light control box was adapted from our previous project [ 64 ] and constructed from white opaque acrylic sheet with outer dimensions 19 × 32 × 15 cm to prevent light penetration from the surroundings ( Figure S1 ). The outer part was covered with PVC sticker sheet.…”
Section: Methodsmentioning
confidence: 99%
“…Apesar de amplamente utilizado, o método de Kjeldahl é moroso, devido às várias etapas envolvidas no procedimento, gera quantidades significativas de resíduos e requer reagentes tóxicos (SÁEZ-PLAZA et al, 2013). Novas propostas vêm sendo exploradas a fim de aprimorar o método de Kjeldahl, como por exemplo, a digestão em escala reduzida (DIDPINRUM et al, 2021). Por ser um procedimento baseado na quantificação de nitrogênio total e não de proteína, a adição de outros compostos nitrogenados não proteicos para restaurar de forma fraudulenta o teor de proteína no alimento, não é identificada por esse método analítico.…”
Section: Lista De Figurasunclassified
“…As principais características analíticas do procedimento proposto para a determinação de proteína em alimentos foram comparadas com outros procedimentos analíticos reportados na literatura (Tabela 5). O procedimento proposto possui faixa de resposta linear comparável ou melhor do que as citadas na Tabela 5 (DIDPINRUM et al, 2021;LAPORTE;PAQUIN, 1999;LÜTHI-PENG;PUHAN, 1999;ROCHA, 2020), além de gerar menores volumes de resíduos por determinação…”
Section: Amostraunclassified
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