A Newly Established ELISA Showing the Effect of the Androgenic Gland on Secondary-Vitellogenic-Specific Protein in the Hemolymph of the Crayfish Cherax quadricarinatus

Sagi, Amir; Khalaila, Isam; Abdu, Uri; Shoukrun, Rami; Weil, Shalva

doi:10.1006/gcen.1999.7277

Cited by 45 publications

(22 citation statements)

References 33 publications

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“…However, the AG-implanted females differed from intersex crayfish in that they lacked a male reproductive system and genital openings. The present findings are in keeping with those of recent studies of AG implantation into immature C. quadricarinatus females (Sagi et al, 1999;Khalaila et al, 2001), as well as with results reported for other crayfish (Nagamine and Knight, 1987;Taketomi et al, 1990;Taketomi and Nishikawa, 1996;Fowler and Leonard, 1999). The fact that the AG-implanted females had higher growth rates than intact females reflects the broad influence of the AG, which extends beyond reproductive processes.…”

Section: Discussionsupporting

confidence: 92%

“…Mean oocyte diameter was calculated from a sample of 15 oocytes per ovary, measured under a light microscope. Secondary vitellogenic cross-reactive proteins in the hemolymph were examined by an enzyme-linked immunosorbent assay (ELISA), with an antibody raised against the specific 106·kDa vitellogenic polypeptide (Sagi et al, 1999). Differences between the IMP and NON females were statistically analyzed using the Mann-Whitney U-test.…”

Section: Morpho-anatomical and Hemolymph Vitellogenic Proteinmentioning

confidence: 99%

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Male-like behavioral patterns and physiological alterations induced by androgenic gland implantation in female crayfish

Barki

Karplus

Khalaila

et al. 2003

Journal of Experimental Biology

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SUMMARY The androgenic gland (AG) has been shown to regulate male sexual differentiation and secondary male characteristics in Crustacea. This study presents for the first time in crustaceans evidence for masculinization effects of the AG on reproductive behavior, in addition to morpho-anatomical and physiological effects. AG implantation into immature female red claw crayfish Cherax quadricarinatus inhibited secondary vitellogenesis and development of the ovaries, as well as morphological traits that facilitate maternal egg brooding; it also caused the appearance of secondary male characteristics. However, primary male characteristics and a masculine reproductive system were not developed. In pair encounters, aggression was substantially lower in interactions between AG-implanted and intact females than in interactions within AG-implanted or intact pairs. Moreover, elements of mating behavior, i.e. male courtship displays and false copulations, were exhibited by AG-implanted females in several encounters with intact females. In addition to known morpho-anatomical and physiological effects of the AG in crustaceans, the present study suggests that the AG has novel effects on the neural network that generates social behavior.

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Section: Discussionsupporting

confidence: 92%

Section: Morpho-anatomical and Hemolymph Vitellogenic Proteinmentioning

confidence: 99%

Male-like behavioral patterns and physiological alterations induced by androgenic gland implantation in female crayfish

Barki

Karplus

Khalaila

et al. 2003

Journal of Experimental Biology

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“…In contrast, the implantation of androgenic gland into female crayfish caused a significant lower concentration of haemolymph vitellogenin. 21 This study demonstrated that haemolymph vitellogenin levels could be used as an indicator for GIH activity, which causes immediate rise of haemolymph vitellogenin levels. The assay for vitellogenin level has several advantages over other previous assay such as suppression of in vitro protein synthesis and determination of incorporated radioactive amino acid into ovarian tissue 15 or inhibition of in vivo ovarian development using GSI.…”

Section: Discussionmentioning

confidence: 71%

“…Similar 19 and different methods of ELISA used for vitellin and vitellogenin determinations have a similar range of sensitivities. [13][14][20][21][22][23] The correlation between haemolymph vitellogenin levels and ovarian index (OI) at various stages of ovarian development was difficult to determined (Fig 2), since the variations of OI and haemolymph vitellogenin levels at each stage of ovarian development from individual prawn were very high especially at stage II, III and IV. In fact, the ovarian development in some prawns were on the second cycle in which most of the nutritional reservoir had been used during the first cycle of ovarian development.…”

Section: Discussionmentioning

confidence: 99%

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Longyant¹,

Sithigorngul²,

Sithigorngul³

et al. 2003

ScienceAsia

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ABSTRACT:Competitive ELISA using a combination of monoclonal antibodies specific to vitellin subunits was used to monitor the fluctuation of haemolymph vitellogenin levels in Penaeus monodon during ovarian development induced by bilateral eyestalk-ablation and to monitor the effect of eyestalk extract injection in prawn with developing ovary. The haemolymph vitellogenin levels were undetectable in the prawn with ovary at the resting stage but elevated sharply when the ovary began to develop, remained high during the ovary developing into ripe stage then fell to the low levels before spawning and spent stages. The result from injection of eyestalk extract into prawn with developing ovary revealed that haemolymph vitellogenin levels elevated sharply within 2 hr, reached the maximal levels and remained high during 4-10 hr, then declined slightly at 24 hr. This response directly depended on the amount of injected eyestalk extract and the response was species specific. The application of eyestalk extract from Metapenaeus affinis demonstrated the same stimulatory effect but was less potent whereas the eyestalk extract from Macrobrachium rosenbergii did not cause any changes in haemolymph vitellogenin levels. Therefore, the assay is specific and provides an indicator to monitor the activity of the putative gonad inhibiting hormone by assaying the alteration of vitellogenin levels.

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“…However, their effects on ovarian growth were different. Individuals in the AG treatment showed no oocyte growth and no secondary oocytes were observed, presumably because of the inhibitory effect of androgenic gland on vitellogenesis (Fowler and Leonard 1999;Sagi et al 1999;Khalaila et al 2001;Barki et al 2003;Cui et al 2005). From a molecular point of view, the inhibitory effects of AGE on vitellogenesis of the crayfish should be tested by ELISA or western blot analysis to indicate the levels of vitellogenin in different groups of samples.…”

Section: Discussionmentioning

confidence: 98%

Sex reversal and growth performance in juvenile females of the freshwater crayfish Cherax quadricarinatus (Parastacidae): effect of increasing temperature and androgenic gland extract in the diet

Bock

Greco

2009

Aquacult Int

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Monosex male culture of C. quadricarinatus is proposed for increasing yields because males reach a larger size at harvest than females. An experiment was done to evaluate the effects of androgenic gland extract and temperature on sex reversal and somatic growth in early juvenile females. Females were exposed to the following experimental conditions: C: control food and temperature maintained at 26 ± 1°C; HT (high temperature): control food and temperature maintained at 28.5 ± 1°C; VHT (very high temperature): control food and temperature maintained at 31 ± 1°C; AG: food with enriched with androgenic gland (1/10 dose for each juvenile per day) and temperature maintained at (26 ± 1)°C. The juveniles were weighed, sexed, and growth increment (GI) and growth rate (GR) were calculated twice a month. The HT and AG groups differed from the C group in GI and GR indicating a greater somatic growth. At the end of the experiment, the HT and AG groups had similar weight but only the HT group had enhanced oocyte diameter, with some vitellogenic oocytes compared to the C group. In the HT group, there was significant sex reversal, as indicated by development of male and intersex male characteristics in different individuals. Both temperature and AG diet have a high potential for culture of this species because of their enhancement of somatic growth; higher temperature increases the proportion of males, a desirable characteristic of cultured populations.

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A Newly Established ELISA Showing the Effect of the Androgenic Gland on Secondary-Vitellogenic-Specific Protein in the Hemolymph of the Crayfish Cherax quadricarinatus

Cited by 45 publications

References 33 publications

Male-like behavioral patterns and physiological alterations induced by androgenic gland implantation in female crayfish

Male-like behavioral patterns and physiological alterations induced by androgenic gland implantation in female crayfish

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Sex reversal and growth performance in juvenile females of the freshwater crayfish Cherax quadricarinatus (Parastacidae): effect of increasing temperature and androgenic gland extract in the diet

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