A nitric acid plant tissue digest method for use with inductively coupled plasma spectrometry

Havlin, J. L.; Soltanpour, P. N.

doi:10.1080/00103628009367096

Cited by 234 publications

(120 citation statements)

References 5 publications

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“…Each yeast culture was inoculated into 300 ml of YPD medium and grown until an A 600 of 1.0 was reached. Yeast cells were harvested and washed and dried at 70°C and subjected to inductively coupled plasma spectrometry analysis (44). To determine the soluble iron concentration, cells were sonicated and broken with a Sonic Dismembrator (Fisher), and the intracellular iron content was examined with a QuantiChron TM iron assay kit (BioAssay Systems, Hayward, CA).…”

Section: Localization Of Atgrxcp-green Fluorescent Protein (Gfp) Fusimentioning

confidence: 99%

AtGRXcp, an Arabidopsis Chloroplastic Glutaredoxin, Is Critical for Protection against Protein Oxidative Damage

Cheng

Liu

Brock

et al. 2006

Journal of Biological Chemistry

138

167

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Glutaredoxins (Grxs) are ubiquitous small heat-stable disulfide oxidoreductases and members of the thioredoxin (Trx) fold protein family. In bacterial, yeast, and mammalian cells, Grxs appear to be involved in maintaining cellular redox homeostasis. However, in plants, the physiological roles of Grxs have not been fully characterized. Recently, an emerging subgroup of Grxs with one cysteine residue in the putative active motif (monothiol Grxs) has been identified but not well characterized. Here we demonstrate that a plant protein, AtGRXcp, is a chloroplast-localized monothiol Grx with high similarity to yeast Grx5. In yeast expression assays, AtGRXcp localized to the mitochondria and suppressed the sensitivity of yeast grx5 cells to H 2 O 2 and protein oxidation. AtGRXcp expression can also suppress iron accumulation and partially rescue the lysine auxotrophy of yeast grx5 cells. Analysis of the conserved monothiol motif suggests that the cysteine residue affects AtGRXcp expression and stability. In planta, AtGRXcp expression was elevated in young cotyledons, green tissues, and vascular bundles. Analysis of atgrxcp plants demonstrated defects in early seedling growth under oxidative stresses. In addition, atgrxcp lines displayed increased protein carbonylation within chloroplasts. Thus, this work describes the initial functional characterization of a plant monothiol Grx and suggests a conserved biological function in protecting cells against protein oxidative damage. Reactive oxygen species (ROS)2 can be formed as by-products in all oxygenic organisms during aerobic metabolism (1). In higher plants, chloroplasts and mitochondria are two major organelles that contribute to production of reactive oxygen species during photosynthesis and carbon metabolism (2, 3). In addition, plants actively generate ROS as signals in response to environmental stresses (3-6). However, because of the cytotoxic and extremely reactive nature of ROS, they can cause wide ranging damage to macromolecules (1, 7-9). To overcome such oxidative damage and control signaling events, plants have orchestrated an elaborate antioxidant network (4).Of those antioxidant systems, the physiological roles of thioredoxins have been intensively studied (10), whereas those of Grxs have not been fully defined (11,12). Grxs are ubiquitous small heat-stable disulfide oxidoreductases, which are conserved in both prokaryotes and eukaryotes (11, 13). Through an active motif, namely the conserved CPYC sequence (a dithiol Grx), they catalyze the reduction of protein disulfides and of GSHprotein mixed disulfides via a dithiol or monothiol mechanism (14, 15). In bacterial, yeast, and mammalian cells, dithiol Grxs appear to be involved in many cellular processes and play an important role in protecting cells against oxidative stresses (16 -18).Besides the dithiol Grxs, a new group of monothiol Grxs has recently been identified in yeast (Grx3, -4, and -5) and bacteria (Grx4) that have a single cysteine residue in the putative active motif (19,20). Yeast Grx5 encodes...

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Section: Localization Of Atgrxcp-green Fluorescent Protein (Gfp) Fusimentioning

confidence: 99%

AtGRXcp, an Arabidopsis Chloroplastic Glutaredoxin, Is Critical for Protection against Protein Oxidative Damage

Cheng

Liu

Brock

et al. 2006

Journal of Biological Chemistry

138

167

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“…Mineral content of cooked meat emulsions was analyzed in duplicate by inductively coupled plasma (ICP) emission spectroscopy after a nitric acid digestion (Havlin and Soltanpour 1980).…”

Section: Mineral Contentmentioning

confidence: 99%

Effects of membrane-filtered soy hull pectin and pre-emulsified fiber/oil on chemical and technological properties of low fat and low salt meat emulsions

2016

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The objectives of this study were to determine efficacy of a membrane filtration in soy hull pectin purification and evaluate combined effects of soy hull pectin and preemulsified fiber/oil (PE) on chemical composition and technological properties of low fat and low salt meat emulsions. Soy hull pectin was purified through two different methods (alcohol-washed (ASP) and membrane-filtered (MSP)). Insoluble soy hull residues after pectin extraction were incorporated with sunflower oil and water for the PE preparation. Meat emulsion was formulated with 58 % pork, 20 % ice, 20 % pork backfat, and 2 % NaCl as control. A total of six low fat and low salt meat emulsions (1 % NaCl and 10 % backfat) was manufactured with 1 % pectin (with/without ASP or MSP) and 10 % PE (with/without). The pectin content of ASP and MSP was 0.84 and 0.64 g L -galacturonic acid/g dry sample, respectively. The inclusion of soy hull pectin caused similar results on chemical composition, color, cooking loss, and texture of the meat emulsions, regardless of the purification method. In addition, positive impacts of the combined treatments with soy hull pectin and PE compared to single treatments on cooking loss and texture of the meat emulsions were observed. Results suggest that membrane filtration could be an effective alternative method to purify pectin, instead of alcohol-washing, and both soluble pectin and insoluble fiber from soy hulls could be used as a functional non-meat ingredient to manufacture various low fat and low salt meat products.

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“…Total N was determined by the Kjeldahl digestion method [15]. Available soil P was determined according to Bray and Kurtz [16], while plant tissue P was analyzed by the wet digestion method [17]. Exchangeable Ca, Mg, and K were extracted using 1 M NH 4 OAc [18] and the cations in the extracts were determined by Atomic Absorption Spectroscopy (AAS).…”

Section: Soil and Plant Analysismentioning

confidence: 99%

Applying Limestone or Basalt in Combination with Bio-Fertilizer to Sustain Rice Production on an Acid Sulfate Soil in Malaysia

et al. 2016

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Abstract:A study was conducted to determine the efficacy of applying ground magnesium limestone (GML) or ground basalt in combination with bio-fertilizer to sustain rice production on an acid sulfate soil in Malaysia. Soils from Kelantan Plains, Malaysia, were treated with GML, ground basalt, bio-fertilizer, GML + bio-fertilizer, and ground basalt + bio-fertilizer (4 t¤ ha ¡1 each). Results showed that soil fertility was improved by applying the soil amendments. GML and basalt contain some Zn and Cu; thus, application of these amendments would increase their contents in the soil needed for the healthy growth of rice. Basalt applied in combination with bio-fertilizer appeared to be the best agronomic option to improve the fertility of acid sulfate soils for sustainable rice production in the long run. In addition to increasing Ca, Mg, Zn, and Cu reserves in the soil, water pH increased and precipitated Al 3+ and/or Fe 2+ . Ground basalt is cheaper than GML, but basalt dissolution in the acidic soil was slow. As such, its ameliorative effects could only be seen significantly from the second season onwards. The specially-formulated bio-fertilizer for alleviating the infertility of acid sulfate soil could also enhance rice growth. The use of the bio-fertilizer fortified with N 2 -fixing bacteria is a green technology that would help reduce NO 3 ¡ and/or NO 2 ¡ pollution and reduce the cost of rice production. The phosphate-solubilizing bacteria (PSB) present in the bio-fertilizer not only increased the available P, but also helped release organic acids that would inactivate Al 3+ and/or Fe 2+ via the process of chelation.

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A nitric acid plant tissue digest method for use with inductively coupled plasma spectrometry

Cited by 234 publications

References 5 publications

AtGRXcp, an Arabidopsis Chloroplastic Glutaredoxin, Is Critical for Protection against Protein Oxidative Damage

AtGRXcp, an Arabidopsis Chloroplastic Glutaredoxin, Is Critical for Protection against Protein Oxidative Damage

Effects of membrane-filtered soy hull pectin and pre-emulsified fiber/oil on chemical and technological properties of low fat and low salt meat emulsions

Applying Limestone or Basalt in Combination with Bio-Fertilizer to Sustain Rice Production on an Acid Sulfate Soil in Malaysia

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