A Nodal/Eph signalling relay drives the transition from apical constriction to apico-basal shortening in ascidian endoderm invagination

Fiúza, Ulla-Maj; Negishi, Takefumi; Rouan, Alice; Yasuo, Hitoyoshi; Lemaire, Patrick

doi:10.1242/dev.186965

Cited by 13 publications

(10 citation statements)

References 81 publications

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“…Importantly, extracellular FGF and ephrin concentrations are assumed to be the same for all cell types. As shown in Fig 2A, good agreement between observed and modeled ERK activation levels can be obtained for the four cell types when considering the values of parameters listed in Table 1 and assuming a linear relationship between computed normalized ERK activity (Erk � ) and dpERK IF signal measured experimentally (Eq (14)).…”

Section: Regulation Of Erk Activity By Fractions Of Cell Surfaces Exp...mentioning

confidence: 58%

“…The experimental data were fitted by a Hill function, best-fit Hill coefficient = 6.09. For the experimental points, the value of Erk � corresponding to each cell was obtained by inversion of Eq (14), with A = 3200 and B = 0. The experimental value of O corresponding to each cell was obtained by inversion of Eq (18), with C = 120 and D = 0.…”

Section: Sensitivity Analysismentioning

confidence: 99%

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Model of neural induction in the ascidian embryo

et al. 2023

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How cell specification can be controlled in a reproducible manner is a fundamental question in developmental biology. In ascidians, a group of invertebrate chordates, geometry plays a key role in achieving this control. Here, we use mathematical modeling to demonstrate that geometry dictates the neural-epidermal cell fate choice in the 32-cell stage ascidian embryo by a two-step process involving first the modulation of ERK signaling and second, the expression of the neural marker gene, Otx. The model describes signal transduction by the ERK pathway that is stimulated by FGF and attenuated by ephrin, and ERK-mediated control of Otx gene expression, which involves both an activator and a repressor of ETS-family transcription factors. Considering the measured area of cell surface contacts with FGF- or ephrin-expressing cells as inputs, the solutions of the model reproduce the experimental observations about ERK activation and Otx expression in the different cells under normal and perturbed conditions. Sensitivity analyses and computations of Hill coefficients allow us to quantify the robustness of the specification mechanism controlled by cell surface area and to identify the respective role played by each signaling input. Simulations also predict in which conditions the dual control of gene expression by an activator and a repressor that are both under the control of ERK can induce a robust ON/OFF control of neural fate induction.

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Section: Regulation Of Erk Activity By Fractions Of Cell Surfaces Exp...mentioning

confidence: 58%

Section: Sensitivity Analysismentioning

confidence: 99%

Model of neural induction in the ascidian embryo

et al. 2023

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“…This value stems from the assumed relatively low concentration of extracellular FGF (K D /5) and, to a lesser extent, from the presence of ephrin (see below). The pharmacological inhibitor of ephrin receptors, NVP-BHG712 (henceforth NVP), has been shown to inhibit Eph signals in ascidian embryos (Fiuza et al, 2020;Williaume et al, 2021). When the model equations were solved with a minimal value of ephrin (0.001) to simulate the presence of the inhibitor, the a6.5>a6.7>a6.6>a6.8 profile of Erk* activation in the four cell types remained unchanged (Fig.…”

Section: Regulation Of Erk Activity By Fractions Of Cell Surfaces Exp...mentioning

confidence: 99%

Model of neural induction in the ascidian embryo

Bettoni

Hudson

Yasuo

et al. 2022

Preprint

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How cell specification can be controlled in a reproducible manner is a fundamental question in development biology. In ascidians, a group of marine invertebrate chordates, geometry plays a key role in achieving this control. Here, we use mathematical modeling to demonstrate that geometry dictates the neural-epidermal cell fate choice in the 32-cell stage ascidian embryo by a two-step process involving first the modulation of ERK signaling and second, the expression of the neural marker gene, Otx. The model describes signal transduction by the ERK pathway that is stimulated by FGF and repressed by ephrin, and ERK-mediated control of Otx gene expression, which involves both an activator and an inhibitor of transcription. Considering the measured area of cell surface contacts with FGF- or ephrin-expressing cells as inputs, the solutions of the model reproduce the experimental observations about ERK activation and Otx expression in the different cells under normal and perturbed conditions. Sensitivity analyses and computations of Hill coefficients allow to quantify the robustness of the specification mechanism controlled by cell surface area and to identify the respective role played by each signaling input. Simulations also predict in which conditions the dual control of gene expression by an activator and an inhibitor that are both under the control of ERK can induce a robust ON/OFF control of neural fate induction

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“…Ascidian gastrulation is initiated by the invagination of 10 endodermal precursor cells between the 64-cell stage and the late 112-cell stage. This process is driven in two steps by a myosin-dependent contraction of the actomyosin network in the absence of endodermal cell division (Sherrard et al, 2010;Winkley et al, 2019;Fiuza et al, 2020).…”

Section: Stagementioning

confidence: 99%

Developmental Table and Three-Dimensional Embryological Image Resource of the Ascidian Ascidiella aspersa

Funakoshi

Shito

Oka

et al. 2021

Front. Cell Dev. Biol.

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Ascidiella aspersa is an ascidian in the class of chordates—the closest relatives of vertebrates. A. aspersa is a potential model organism for bio-imaging studies due to its extremely transparent embryos as well as is a globally distributed cosmopolitan species. However, there is no standard developmental table for this organism. Here, as a first step to establish A. aspersa as a model organism, we report a standard developmental table as a web-based digital image resource. This resource used confocal laser scanning microscopy to scan more than 3,000 cross-sectional images and 3D-reconstructed images of A. aspersa embryos during embryogenesis. With reference to the standardized developmental table of Ciona intestinalis type A, 26 different developmental stages (Stages 1–26) from fertilized eggs to hatched larvae were redefined for A. aspersa. Cell lineages up to the cleavage period were annotated: The cleavage patterns, the embryonic morphology, and the developmental time were then compared with Ciona. We found that the cleavage patterns and developmental time up to the neurula period in A. aspersa were extremely conserved versus. Ciona. The ratio of the trunk and tail length in the tailbud period were smaller than Ciona indicating a relatively short tail. In addition, the timing of the bending of the tail is earlier than Ciona. This A. aspersa standard 3D digital resource is essential for connecting different omics data to different spatiotemporal hierarchies and is useful for a system-level understanding of chordate development and evolution.

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A Nodal/Eph signalling relay drives the transition from apical constriction to apico-basal shortening in ascidian endoderm invagination

Cited by 13 publications

References 81 publications

Model of neural induction in the ascidian embryo

Model of neural induction in the ascidian embryo

Model of neural induction in the ascidian embryo

Developmental Table and Three-Dimensional Embryological Image Resource of the Ascidian Ascidiella aspersa

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