A nonintrinsic regional basis for increased infrarenal aortic MMP-9 expression and activity

Ailawadi, Gorav; Knipp, Brian S.; Lu, Guanyi; Roelofs, Karen J.; Ford, John W.; Hannawa, Kevin K.; Bishop, Keith; Thanaporn, Porama; Henke, Peter K.; Stanley, James C.; Upchurch, Gilbert R.

doi:10.1067/mva.2003.163

Cited by 40 publications

(29 citation statements)

References 37 publications

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“…Previous work by Ailawadi et al suggests that ED-1-positive cells are increased after transplantation compared with native aortic explants. 21 The lack of CD3-positive lymphocytes and the few architectural differences Figure 3. Estradiol-treated male aortas subjected to elastase perfusion.…”

Section: Discussionmentioning

confidence: 99%

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Gender Differences in Experimental Aortic Aneurysm Formation

Ailawadi

Eliason

Roelofs

et al. 2004

ATVB

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Objective-It is hypothesized that a male predominance, similar to that in humans, persists in a rodent model of experimental abdominal aortic aneurysm (AAA) via alterations in matrix metalloproteinases (MMPs). Methods and Results-Group I experiments were as follows: elastase perfusion of the infrarenal aorta was performed in male (M) and female (F) rats. At 14 days, aortas were harvested for immunohistochemistry, real-time polymerase chain reaction (PCR), and zymography. Group II experiments were the following: abdominal aorta was transplanted from F or M donors into F or M recipients. At 14 days, rodents that had undergone transplantation underwent elastase perfusion. In group III, male rats were given estradiol or sham 5 days before elastase perfusion. In group I, M rats had larger AAAs with higher frequency than did F rats. M rat aortas had more significant macrophage infiltrates and increased matrix metalloproteinase (MMP)-9 production and activity. In group II, M-to-M aortic transplants uniformly developed aneurysms after elastase perfusion, whereas F-to-F aortic transplants remained resistant to aneurysm formation. F aortas transplanted into M recipients, however, lost aneurysm resistance. In group III, estradiol-treated rats demonstrated smaller aneurysms and less macrophage infiltrate and MMP-9 compared with M controls after elastase. Conclusions-These data provide evidence of gender-related differences in AAA development, which may reflect an estrogen-mediated reduction in macrophage MMP-9 production.

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Section: Discussionmentioning

confidence: 99%

“…21 Briefly, male and female donor rats were anesthetized and the abdominal aortas isolated. Donor rats were anticoagulated with 300 U of heparin and the abdominal aorta was rapidly removed and placed in cold 0.9% normal saline.…”

Section: Aortic Transplantationmentioning

confidence: 99%

Gender Differences in Experimental Aortic Aneurysm Formation

Ailawadi

Eliason

Roelofs

et al. 2004

ATVB

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171

147

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“…92 This phenomenon has not been studied in experimental aneurysm formation. However, when Ailawadi et al 86 transplanted syngeneic thoracic aortic segments into the abdominal aorta, variations in matrix metalloproteinase-9 expression appeared to be related to regional factors and not intrinsic aortic wall factors.…”

Section: Smc Lineage and Diversitymentioning

confidence: 99%

“…18 For example, in rats, the expression and activity of matrix metalloproteinase-9 is greater in the abdominal than in the thoracic aorta. 86 Localized adverse hemodynamic conditions have been implicated in the pathogenesis of cerebral aneurysms. 19,87 Provenance of medial SMC influences response to multiple stimuli Localised inflammation Site Figure 6.…”

Section: Role Of Bifurcationsmentioning

confidence: 99%

Site Specificity of Aneurysmal Disease

2010

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“…The thoracic and abdominal aorta contain cells of distinct embryologic origins [3]. Additionally, the thoracic aorta has greater elastin content and contains more prominent vaso vasora than the abdominal aorta [4][5][6]. Current nonsurgical therapy for AAA and TAA is aimed at preventing further dilation and includes risk factor reduction and b-adrenergic blockade (for TAA), both with limited efficacy [1,7].…”

mentioning

confidence: 99%